A5068392456- Mitochondrial Function and Pathology
- Alzheimer's disease research and treatments
- Genetics and Neurodevelopmental Disorders
- Neuroscience and Neuropharmacology Research
- Cholinesterase and Neurodegenerative Diseases
- Wnt/β-catenin signaling in development and cancer
- Cell death mechanisms and regulation
- ATP Synthase and ATPases Research
- Neurogenesis and neuroplasticity mechanisms
- Trace Elements in Health
- Metabolism and Genetic Disorders
- Computational Drug Discovery Methods
- Amino Acid Enzymes and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Ion channel regulation and function
- Biochemical effects in animals
- Nanocluster Synthesis and Applications
- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Nerve injury and regeneration
- Folate and B Vitamins Research
- Nuclear Structure and Function
- Adipose Tissue and Metabolism
- Inflammasome and immune disorders
- Nicotinic Acetylcholine Receptors Study
Universidad Mayor
2018-2024
Université Toulouse III - Paul Sabatier
2017-2023
Centre de Recherches sur la Cognition Animale
2017-2023
Centre National de la Recherche Scientifique
2017-2023
Université de Toulouse
2017-2023
Center for Climate and Resilience Research
2014-2023
Buck Institute for Research on Aging
2019-2022
Creative Commons
2022
Pontificia Universidad Católica de Chile
2008-2021
Abstract The presence of amyloid-β (Aβ) deposits in selected brain regions is a hallmark Alzheimer's disease (AD). amyloid have "chaperone molecules" which play critical roles formation and toxicity. We report here that treatment rat hippocampal neurons with Aβ-acetylcholinesterase (Aβ-AChE) complexes induced neurite network dystrophia apoptosis. Moreover, the Aβ-AChE sustained increase intracellular Ca 2+ as well loss mitochondrial membrane potential. oligomers complex also higher...
L-methionine, the principal sulfur-containing amino acid in proteins, plays critical roles cell physiology as an antioxidant and breakdown of fats heavy metals. Previous studies suggesting use L-methionine a treatment for depression other diseases indicate that it might also improve memory propose role brain function. However, some evidence indicates excess methionine can be harmful increase risk developing Type-2 diabetes, heart diseases, certain types cancer, alterations such...
Alzheimer's disease (AD) is the most common type of age-related dementia. The characterized by a progressive loss cognitive abilities, severe neurodegeneration, synaptic and mitochondrial dysfunction. Wnt signaling pathway participates in development central nervous system growing evidence indicates that Wnts also regulate function adult system. We report here, indirect activation canonical Wnt/β-catenin using Bromoindirubin-30-Oxime (6-BIO), an inhibitor glycogen synthase kinase-3β,...
Axonal degeneration, which contributes to functional impairment in several disorders of the nervous system, is an important target for neuroprotection. Several individual factors and subcellular events have been implicated axonal but researchers so far unable identify integrative signaling pathway activating this self-destructive process. Through pharmacological genetic approaches, we tested whether necroptosis, a regulated cell-death mechanism pathogenesis neurodegenerative diseases,...
Calcium/calmodulin-dependent protein kinase IV (CaMKIV) plays a key role in the regulation of calcium-dependent gene expression. The expression CaMKIV and activation CREB regulated genes are involved memory neuronal survival. We report here that: (a) bioinformatic analysis 15,476 promoters human genome predicted several Wnt target genes, being very interesting candidate; (b) promoter contains TCF/LEF transcription motifs similar to those present genes; (c) biochemical studies indicate that...
The Wnt signaling pathway plays an important role in developmental processes, including embryonic patterning, cell specification, and polarity. components participate the development of central nervous system, growing evidence indicates that this also regulates function adult system. In study, we report Wnt-5a, a noncanonical ligand, is potent activator mitochondrial dynamics induces acute fission fusion events mitochondria rat hippocampal neurons. effect Wnt-5a was inhibited presence sFRP,...
Age is the main risk factor for development of neurodegenerative diseases. In aged brain, axonal degeneration an early pathological event, preceding neuronal dysfunction, and cognitive disabilities in humans, primates, rodents, invertebrates. Necroptosis mediates injured axons, but whether necroptosis triggers neurodegeneration impairment along aging unknown. Here, we show that loss necroptotic effector Mlkl was sufficient to delay age-associated neuroinflammation, protecting against...
Alzheimer's disease (AD) is a neurodegenerative disorder mainly known for synaptic impairment and neuronal cell loss, affecting memory processes. Beside these damages, mitochondria have been implicated in the pathogenesis of AD through induction mitochondrial permeability transition pore (mPTP). The mPTP non-selective that formed under apoptotic conditions, disturbing structure thus, viability. In AD, Aβ oligomers (Aβos) favor opening pore, activating mitochondria-dependent death cascades....
To examine the role of enzyme methionine sulfoxide reductase A-1 (MSRA-1) in amyloid-β peptide (Aβ)-peptide aggregation and toxicity vivo, using a Caenorhabditis elegans model human amyloidogenic disease inclusion body myositis.MSRA-1 specifically reduces oxidized methionines proteins. Therefore, deletion msra-1 gene was introduced into transgenic C. worms that express Aβ-peptide muscle cells to prevent reduction In constitutive Aβ strain lacks MSRA-1, number amyloid aggregates decreases...
Alzheimer’s Disease (AD) is the primary cause of dementia among elderly population. Elevated plasma levels homocysteine (HCy), an amino acid derived from methionine metabolism, are considered a risk factor and biomarker AD other types dementia. An increase in HCy mostly consequence high and/or low vitamin B intake diet. Here, we studied effects physiological pathophysiological concentrations on oxidative stress, synaptic protein levels, activity mice hippocampal slices. We also vitro...
Excitotoxicity and neuronal cell death: Glutamate is the main excitatory neurotransmitter of central nervous system functionally involved in most brain activities, including development, synaptic plasticity, learning memory. Excitatory transmission primarily mediated by ligand-gated ion channels, α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionic acid (AMPA), N-methyl-D-aspartate (NMDA) kainate receptors. Activation glutamate receptors, particularly NMDA usually leads to calcium influx, which...
Abstract Pathogenic variants of OPA1 , which encodes a dynamin GTPase involved in mitochondrial fusion, are responsible for spectrum neurological disorders sharing optic nerve atrophy and visual impairment. To gain insight on neuronal specificity, we performed targeted metabolomics rat cortical neurons with expression inhibited by RNA interference. Of the 103 metabolites accurately measured, univariate analysis including Benjamini-Hochberg correction revealed 6 significantly different...
Mitochondria are integrative hubs central to cellular adaptive pathways. Such pathways critical in highly differentiated postmitotic neurons, the plasticity of which sustains brain function. Consequently, defects mitochondria and their dynamics appear instrumental neurodegenerative diseases may also participate cognitive impairments. To directly test this hypothesis, we analyzed performances a mouse mitochondria-based disease model, because haploinsufficiency mitochondrial optic atrophy type...
Abstract Wnt signaling plays a key role in neurodevelopment and neuronal maturation. Specifically, Wnt5a stimulates postsynaptic assemblies, increases glutamatergic neurotransmission and, through calcium signaling, generates nitric oxide (NO). Trying to unveil the molecular pathway triggering these effects, we found that treatment induces time-dependent length of density (PSD), elicits novel synaptic contacts facilitates F-actin flow both vitro ex vivo models. These effects were partially...
Carbon dots (CDs) have been quickly extended for nanomedicine uses because of their multiple applications, such as bioimaging, sensors, and drug delivery. However, the interest in increasing photoluminescence properties is not always accompanied by cytocompatibility. Thus, a knowledge gap exists regarding interactions with biological systems linked to selected formulations synthesis methods. In this work, we developed carbon based on poly (ethylene imine) (PEI) chitosan (CS) using microwave...
Several decades have passed since programmed-cell death (PCD) was identified. Apoptosis first defined by Kerr in 1972, and later described the Nobel Prices Physiology or Medicine 2002, Sydney Brenner, John Sulston Robert Horwitz, who genetic regulators of apoptosis (Diamantis et al., 2008). However, it 1858 when German pathologist biologist Rudolf Virchow identified for time phenomenon apoptosis, which he named necrobiosis, arguing that this form cell completely different from uncontrolled...
Corneal diseases represent a significant global health challenge, often resulting in blindness, for which penetrating keratoplasty is the clinical gold standard. However, cases involving compromised ocular surfaces or graft failure, osteo-odonto keratoprosthesis (OOKP) emerges as vital yet costly and complex alternative. Thus, there an urgent need to introduce soft biomaterials that mimic corneal tissue, considering its translation’s physicochemical, biological, economic costs. This study...