A5009141968- Advanced Proteomics Techniques and Applications
- Chronic Kidney Disease and Diabetes
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Pediatric Urology and Nephrology Studies
- Renal Diseases and Glomerulopathies
- Renal and related cancers
- Mass Spectrometry Techniques and Applications
- Dialysis and Renal Disease Management
- Acute Kidney Injury Research
- Metabolomics and Mass Spectrometry Studies
- Urological Disorders and Treatments
- Biotin and Related Studies
- Peptidase Inhibition and Analysis
- Renin-Angiotensin System Studies
- Mast cells and histamine
- Advanced Glycation End Products research
- Muscle and Compartmental Disorders
- Blood Coagulation and Thrombosis Mechanisms
- Renal and Vascular Pathologies
- Pregnancy and preeclampsia studies
- Biomedical Text Mining and Ontologies
- Microbial bioremediation and biosurfactants
- Semantic Web and Ontologies
- Parathyroid Disorders and Treatments
- Pancreatic function and diabetes
Inserm
2016-2025
Institut des Maladies Métaboliques et Cardiovasculaires
2016-2025
Université de Toulouse
2008-2025
Université Toulouse III - Paul Sabatier
2015-2024
Limousin Regional Council
2024
Hôpital Tenon
2023
Sorbonne Université
2023
Assistance Publique – Hôpitaux de Paris
2023
Hôpital Rangueil
2004-2021
Centre Hospitalier Universitaire de Toulouse
1998-2021
Because of its availability, ease collection, and correlation with physiology pathology, urine is an attractive source for clinical proteomics/peptidomics. However, the lack comparable data sets from large cohorts has greatly hindered development proteomics. Here, we report establishment a reproducible, high resolution method peptidome analysis naturally occurring human urinary peptides proteins, ranging 800 to 17,000 Da, using samples 3,600 individuals analyzed by capillary electrophoresis...
Recommendations about structuring proteomic biomarker studies should increase the probability that such markers will be clinically useful.
The aim of this manuscript is to initiate a constructive discussion about the definition clinical proteomics, study requirements, pitfalls and (potential) use. Furthermore, we hope stimulate proposals for optimal use future opportunities seek unification approaches in proteomic studies. We have outlined our collective views basic principles that should be considered studies, including sample selection, choice technology appropriate quality control, need collaborative interdisciplinary...
Progressive CKD is generally detected at a late stage by sustained decline in eGFR and/or the presence of significant albuminuria. With aim early and improved risk stratification patients with CKD, we studied urinary peptides large cross-sectional multicenter cohort 1990 individuals, including 522 follow-up data, using proteome analysis. We validated that previously established multipeptide biomarker classifier performed significantly better detecting predicting progression than current...
Prenatal discovery of fetal bilateral hyperechogenic kidneys is very stressful for pregnant women and their family, accurate diagnosis the cause moderate forms this pathology difficult. Hepatocyte nuclear factor-1beta that encoded by TCF2 gene involved in embryonic development kidneys. Sixty-two pregnancies with including 25 fetuses inaccurate were studied. anomalies detected 18 (29%) these 62 patients, 15 patients presented a complete heterozygous deletion gene. Family screening revealed de...
Abstract Owing to its availability, ease of collection, and correlation with pathophysiology diseases, urine is an attractive source for clinical proteomics. However, many proteomic studies have had only limited impact, due factors such as modest numbers subjects, absence disease controls, small defined biomarkers, diversity analytical platforms. Therefore, it difficult merge biomarkers from different into a broadly applicable human urinary proteome database. Ideally, the methodology...
Tubulointerstitial fibrosis in chronic renal disease is strongly associated with progressive loss of function. We studied the potential involvement lysophosphatidic acid (LPA), a growth factor–like phospholipid, and its receptors LPA1–4 development tubulointerstitial (TIF). Renal was induced mice by unilateral ureteral obstruction (UUO) for up to 8 d, kidney explants were prepared from distal poles measure LPA release into conditioned media. After obstruction, extracellular increased...
The bradykinin B1-receptor is strongly upregulated under chronic inflammatory conditions. However, the mechanism and reason are not known. Because a better understanding of upregulation will help in its potential importance inflammation, we have studied molecular cultured human lung fibroblasts (IMR 90) response to IL-1beta B1-agonist [des-Arg10]-kallidin. We show that treatment IMR 90 cells by stimulates expression both mRNA protein. latter was Western blot analysis using antipeptide...
While large numbers of proteomic biomarkers have been described, they are generally not implemented in medical practice. We investigated the reasons for this shortcoming, focusing on hurdles downstream biomarker verification, and describe major obstacles possible solutions to ease valid implementation. Some problems lie suboptimal discovery validation, especially lack validated platforms with well-described performance characteristics support qualification. These issues acknowledged being...
Abstract Purpose : Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose of this study the development well‐characterized “real life” sample that can be used reference standard in urine clinical studies. Experimental design We report on generation male and female samples are extensively characterized by different platforms methods (CE‐MS, LC‐MS, LC‐MS/MS, 1‐D gel analysis combination with nano‐LC MS/MS (using LTQ‐FT ultra), 2‐DE‐MS) their proteome peptidome. In...
Rhabdomyolysis can be life threatening if complicated by AKI. Macrophage infiltration has been observed in rat kidneys after glycerol-induced rhabdomyolysis, but the role of macrophages rhabdomyolysis-induced AKI remains unknown. Here, a patient diagnosed with we detected substantial macrophage kidney. In mouse model AKI, diverse renal phenotypes were depending on stage disease. Two days F4/80(low)CD11b(high)Ly6b(high)CD206(low) kidney dominant, whereas day 8,...
Background. Chronic kidney disease (CKD) progression is currently assessed by a decline in estimated glomerular filtration rate (eGFR) and/or an increase urinary albumin excretion (UAE). However, these markers are considered either to be late-stage or have low sensitivity specificity. In this study, we investigated the performance of proteome-based classifier CKD273, compared with UAE, number different narrow ranges CKD severity, each range separated eGFR 10 mL/min/1.73 m2. Methods. A total...
Diabetic nephropathy (DN) is one of the major late complications diabetes. Treatment aimed at slowing down progression DN available but methods for early and definitive detection are currently lacking. The 'Proteomic prediction Renin angiotensin aldosterone system Inhibition prevention Of diabetic nephRopathy In TYpe 2 patients with normoalbuminuria trial' (PRIORITY) aims to evaluate in type diabetes (T2D) using a urinary proteome-based classifier (CKD273).In this ancillary study recently...
BackgroundWhile much has been learned about the epidemiology and treatment of end-stage renal disease (ESRD) in last 30 years, chronic kidney (CKD) before less investigated. Not enough is known factors associated with CKD progression complications, as well its transition to ESRD. We designed CKD-renal information network (REIN) cohort provide a research platform address these key questions assess clinical practices costs patients moderate or advanced CKD.
In spite of its invasive nature and risks, kidney biopsy is currently required for precise diagnosis many chronic diseases (CKDs). Here, we explored the hypothesis that analysis urinary proteome can discriminate different types CKD irrespective underlying mechanism disease.We used data from analyses 1180 urine samples patients with CKD, generated by capillary electrophoresis coupled to mass spectrometry. A set 706 served as discovery cohort, 474 were independent validation. For each type,...
Angiotensin-converting enzyme (ACE) inhibitors reduce the progression of various fibrotic renal diseases both in humans and animal models. Unilateral ureteral obstruction (UUO) is an model accelerated tubulointerstitial fibrosis that attenuated by ACE inhibition. Although increase bradykinin concentrations addition to their effect on angiotensin II formation, role has not been studied. We show here genetic ablation (B2(-/-) mice) or pharmacological blockade B2 receptor increases UUO-induced...
Angiotensin-converting enzyme (ACE) inhibitors reduce the progression of various fibrotic renal diseases both in humans and animal models. Unilateral ureteral obstruction (UUO) is an model accelerated tubulointerstitial fibrosis that attenuated by ACE inhibition. Although increase bradykinin concentrations addition to their effect on angiotensin II formation, role has not been studied. We show here genetic ablation (B2–/– mice) or pharmacological blockade B2 receptor increases UUO-induced...