A5038669347- Advanced Proteomics Techniques and Applications
- Lysosomal Storage Disorders Research
- Alzheimer's disease research and treatments
- Mass Spectrometry Techniques and Applications
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cerebrovascular and genetic disorders
- Mitochondrial Function and Pathology
- Pregnancy and preeclampsia studies
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Digestive system and related health
- Advanced MEMS and NEMS Technologies
- Diet and metabolism studies
- Estrogen and related hormone effects
- Sphingolipid Metabolism and Signaling
- Computational Drug Discovery Methods
- Drug Transport and Resistance Mechanisms
- Mechanical and Optical Resonators
- Microfluidic and Capillary Electrophoresis Applications
- Lipoproteins and Cardiovascular Health
- Metabolomics and Mass Spectrometry Studies
- Ion-surface interactions and analysis
- Cellular transport and secretion
- Amino Acid Enzymes and Metabolism
- Protease and Inhibitor Mechanisms
University of Helsinki
2015-2025
University of California, Santa Barbara
2024-2025
University Hospital of Bern
2013-2024
Klinik für Frauenheilkunde
2023-2024
University of Bern
2014-2023
Temple University
2021
Pasteur Institute of Iran
2021
Shahid Beheshti University
2021
Motamed Cancer Institute
2021
Academic Center for Education, Culture and Research
2021
Amyloid β-peptide (Aβ) is a major fibrillar component of neuritic plaques in Alzheimer's disease (AD) brains and related to the pathogenesis disease. We hypothesized that amyloid formation could be inhibited by peptides homologous Aβ (position 17-21) with similar degree hydrophobicity, but very low propensity adopt β-sheet conformation incorporating proline residues (anti-β-sheet or inhibitors). An 11-residue peptide these characteristics binds Aβ, inhibits fibril partially disaggregates...
Formaldehyde dehydrogenase; Alcohol dehydrogenase, class III; Sequence homology; Amino acid sequence
Recommendations about structuring proteomic biomarker studies should increase the probability that such markers will be clinically useful.
Serum amyloid A (SAA) is an acute-phase protein, the serum levels of which can increase up to 1000-fold during inflammation. SAA has a pathogenic role in A-type amyloidosis, and increased correlate with risk for cardiovascular diseases. IL-1β key proinflammatory cytokine, its secretion strictly controlled by inflammasomes. We studied regulation production activation inflammasome cascade human mouse macrophages, as well THP-1 cells. could provide signal induction pro-IL-1β expression...
Low-density lipoprotein (LDL) particles cause atherosclerotic cardiovascular disease (ASCVD) through their retention, modification, and accumulation within the arterial intima. High plasma concentrations of LDL drive this disease, but quality may also contribute. Here, we focused on intrinsic propensity to aggregate upon modification. We examined whether inter-individual differences in are linked with lipid composition coronary artery (CAD) death, basic mechanisms for plaque growth...
Amphoterin is a heparin-binding protein that developmentally regulated in brain and functionally involved neurite outgrowth. Unexpectedly, amphoterin has high mobility group 1 (HMG1)-type sequence. In the present study we have expressed cDNA baculovirus vector produced antibodies against recombinant several synthetic peptides. It was found encodes 30-kDa form of isolated from tissues, whereas co-purifying 28- 29-kDa proteins (p28 p29) closely related but distinct primary structures. Partial...
We have isolated storage cytosomes from brain tissue of patients with infantile neuronal ceroid‐lipofuscinosis. The purified bodies were subjected to compositional analysis which revealed a high content proteins, accounting for 43% dry weight. Saposins A and D, also known as sphingolipid activator proteins (SAPs), shown constitute major portion the accumulated protein using gel electrophoresis sequence analysis. This is first time that saposins been found be stored in any form
Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is the most common hereditary vascular dementia. It caused by mutations in NOTCH3 gene, which encodes a large transmembrane receptor Notch3. The key pathological finding accumulation of granular osmiophilic material (GOM), contains extracellular domains Notch3, on degenerating smooth muscle cells (VSMCs). GOM has been considered specifically diagnostic for CADASIL, but reports sensitivity...
Notch signaling is critically important for proper architecture of the vascular system, and mutations in NOTCH3 are associated with CADASIL, a stroke dementia syndrome smooth muscle cell (VSMC) dysfunction. In this report, we link to platelet-derived growth factor (PDGF) signaling, key determinant VSMC biology, show that PDGF receptor ( PDGFR )-β novel immediate target gene. -β expression was upregulated by ligand induction or activated forms receptor. Moreover, upregulation response...
SETBP1 variants occur as somatic mutations in several hematological malignancies such atypical chronic myeloid leukemia and de novo germline the Schinzel-Giedion syndrome. Here we show that binds to gDNA AT-rich promoter regions, causing activation of gene expression through recruitment a HCF1/KMT2A/PHF8 epigenetic complex. Deletion two AT-hooks abrogates binding impairs target upregulation. Genes controlled by MECOM are significantly upregulated leukemias containing mutations. Gene ontology...
Recently, the human gene encoding erythroid-specific delta-aminolevulinate synthase was localized to chromosomal region Xp21-Xq21, identifying this as logical candidate for enzymatic defect causing "X-linked" sideroblastic anemia. To investigate hypothesis, 11 exonic coding regions of were amplified and sequenced from a 30-year-old Chinese male with pyridoxine-responsive form X-linked A single T----A transition found in codon 471 highly conserved exon 9, resulting an Ile----Asn substitution....
Eight human liver UDP-glucuronosyltransferases (UGTs) were expressed in baculovirus-infected insect cells as fusion proteins carrying a short C-terminal extension that ends with 6 histidine residues (His tag). The activity of recombinant UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT2B4, UGT2B7, and UGT2B15 was almost fully inhibited by 0.2% Triton X-100. In the case UGT1A9, however, glucuronidation alpha-naphthol scopoletin resistant to such inhibition, whereas entacapone several other aglycones...
A cDNA clone for cystathionine gamma-lyase was isolated from a rat library in lambda gt11 by screening with monospecific antiserum. The identity of this clone, containing 600 bp proximal to the 3'-end gene, confirmed positive hybridization selection. Northern-blot showed expected higher abundance corresponding mRNA liver than brain. Two further clones plasmid pcD were colony first and found contain inserts 1600 1850 bp. One these as encoding two independent pools oligodeoxynucleotides...
Amyloid fibrils were isolated from the kidney of a patient with Finnish hereditary amyloidosis. After solubilization in guanidine‐HCl, fractionation by gel filtration, and purification reverse‐phase high‐performance liquid chrornatography, homogeneous amyloid protein an apparent M r 9000 was obtained. The subjected to enzymatic digestion trypsin endoproteinase Lys‐C. amino acid sequences determined for 6 released peptides they all found be identical reported, deduced primary structure human...
The abundant proteolytic plasminogen (Plg)/plasmin system is important in several physiological functions mammals and also engaged by a number of pathogenic microbial species to increase tissue invasiveness or obtain nutrients. This paper reports that commensal bacterium, Lactobacillus crispatus, interacts with the Plg system. Strain ST1 L. crispatus enhanced activation human tissue-type activator (tPA), whereas enhancement urokinase-mediated was lower. cells bound Plg, plasmin tPA only...
Background Narcolepsy results from immune-mediated destruction of hypocretin secreting neurons in hypothalamus, however the triggers and disease mechanisms are poorly understood. Vaccine-attributable risk narcolepsy reported so far with AS03 adjuvanted H1N1 vaccination Pandemrix has been manifold compared to Arepanrix, which contained differently produced viral antigen preparation. Hence, antigenic differences antibody response these vaccines were investigated. Methods Findings Increased...
Western-style diets (WD) high in fat and scarce fiber vitamin D increase risks of colorectal cancer. Here, we performed a long-term diet study mice to follow tumorigenesis characterize structural metabolic changes colon mucosa associated with WD predisposition increased tumor numbers, proteomic analysis indicated severe deregulation intracellular bile acid (BA) homeostasis activation cell proliferation. also crypt depth Despite luminal BA, colonocytes from WD-fed exhibited decreased...
Objective Increased risk for atherosclerosis is associated with infectious diseases including periodontitis. Natural IgM antibodies recognize pathogen-associated molecular patterns on bacteria, and oxidized lipid protein epitopes low-density lipoprotein (LDL) apoptotic cells. We aimed to identify periodontal pathogen Porphyromonas gingivalis recognized by natural binding malondialdehyde (MDA) modified LDL. Methods Results Mouse monoclonal (MDmAb) specific MDA-LDL P. flow cytometry...