A5089088505- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Digestive system and related health
- Plant Molecular Biology Research
- Diet and metabolism studies
- Drug Transport and Resistance Mechanisms
- Plant Disease Resistance and Genetics
- Plant Gene Expression Analysis
- Mitochondrial Function and Pathology
- Helicobacter pylori-related gastroenterology studies
- Light effects on plants
- RNA modifications and cancer
- Plant Pathogenic Bacteria Studies
- Phytochemicals and Antioxidant Activities
- Potato Plant Research
- Cancer, Lipids, and Metabolism
- Polysaccharides and Plant Cell Walls
- Plant biochemistry and biosynthesis
- Plant Pathogens and Resistance
- Plant responses to water stress
- Redox biology and oxidative stress
- Plant Virus Research Studies
- Horticultural and Viticultural Research
University of Helsinki
2015-2024
University of Eastern Finland
2011-2022
Finland University
2020
University of Glasgow
2012
University of Tartu
2011-2012
Massey University
2012
University of Turku
2009-2011
University of Münster
2011
Agrifood Research Finland
2009
Summary WRKY transcription factors ( TF s) have been mainly associated with plant defense, but recent studies suggested additional roles in the regulation of other physiological processes. Here, we explored possible contribution two related group III s, 70 and 54, to osmotic stress tolerance. These s are positive regulators co‐operate as negative salicylic acid SA ) biosynthesis senescence. We employed single double mutants wrky54 wrky70 , well a WRKY70 overexpressor line, explore role these...
Reactive oxygen species (ROS)-dependent signaling pathways from chloroplasts and mitochondria merge at the nuclear protein RADICAL-INDUCED CELL DEATH1 (RCD1). RCD1 interacts in vivo suppresses activity of transcription factors ANAC013 ANAC017, which mediate a ROS-related retrograde signal originating mitochondrial complex III. Inactivation leads to increased expression dysfunction stimulon (MDS) genes regulated by ANAC017. Accumulating MDS gene products, including alternative oxidases...
Abstract Photomorphogenic responses triggered by low fluence rates of ultraviolet B radiation (UV-B; 280–315 nm) are mediated the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8). Beyond our understanding molecular mechanisms perception UVR8, there is still limited information on how UVR8 pathway functions under natural sunlight. Here, wild-type Arabidopsis (Arabidopsis thaliana) and uvr8-2 mutant were used in an experiment outdoors where UV-A (315–400 irradiances attenuated using plastic...
Abstract Reactive oxygen species (ROS) are ubiquitous signaling molecules in plant stress and development. To gain further insight into the transcriptional response to apoplastic ROS, phytotoxic atmospheric pollutant ozone was used as a model ROS inducer Arabidopsis (Arabidopsis thaliana) gene expression analyzed with microarrays. In contrast increase via hormones salicylic acid, abscisic jasmonic acid (JA), ethylene, treatment caused auxin be transiently suppressed, which confirmed DR5-uidA...
Abstract Chloroplast NADPH-thioredoxin reductase (NTRC) belongs to the thioredoxin systems that control crucial metabolic and regulatory pathways in plants. Here, by characterization of T-DNA insertion lines NTRC gene, we uncover a novel connection between chloroplast thiol redox regulation photoperiodic growth Arabidopsis (Arabidopsis thaliana). Transcript metabolite profiling revealed severe developmental defects ntrc plants grown under short 8-h light period. Besides reduced chlorophyll...
The Arabidopsis thaliana F-box protein MORE AXILLARY GROWTH2 (MAX2) has previously been characterized for its role in plant development. MAX2 appears essential the perception of newly phytohormone strigolactone, a negative regulator polar auxin transport Arabidopsis. A reverse genetic screen mutants altered their stress responses identified as component defense. Here we show that contributes to resistance against pathogenic bacteria. Interestingly, max2 mutant plants showed increased...
Oligogalacturonides (OGs) are important components of damage-associated molecular pattern (DAMP) signaling and influence growth regulation in plants. Recent studies have focused on the impact long OGs (degree polymerization (DP) from 10–15), demonstrating induction plant defense resulting enhanced defenses to necrotrophic pathogens. To clarify role trimers (trimeric OGs, DP3) DAMP their regulation, we performed a transcriptomic analysis through RNA sequencing Arabidopsis thaliana exposed...
The biosynthesis of anthocyanins has been shown to be influenced by light quality. However, the molecular mechanisms underlying light-mediated regulation fruit anthocyanin are not well understood. In this study, we analysed effects supplemental red and blue on in non-climacteric bilberry (Vaccinium myrtillus L.). After 6 days continuous irradiation during ripening, both elevated concentration anthocyanins, up 12- 4-folds, respectively, compared control. Transcriptomic analysis ripening...
Plants synthesize phenolic compounds in response to certain environmental signals or stresses. One large group of phenolics, flavonoids, is considered particularly responsive ultraviolet (UV) radiation. However, here we demonstrate that solar blue light stimulates flavonoid biosynthesis the absence UV-A and UV-B We grew pea plants (Pisum sativum cv. Meteor) outdoors, Finland during summer, under five types filters differing their spectral transmittance. These were used (1) attenuate UV-B;...
Plant responses to changes in environmental conditions are mediated by a network of signaling events leading downstream responses, including gene expression and activation cell death programs. Arabidopsis thaliana RADICAL-INDUCED CELL DEATH1 (RCD1) has been proposed regulate plant stress protein-protein interactions with transcription factors. Furthermore, the rcd1 mutant defective control response apoplastic reactive oxygen species (ROS). Combining transcriptomic functional genomics...
Abstract Light is an important environmental factor that modulates acclimation strategies and defense responses in plants. We explored the functional role of regulatory subunit B′γ (B′γ) protein phosphatase 2A (PP2A) light-dependent stress Arabidopsis (Arabidopsis thaliana). The predominant form PP2A consists catalytic C, scaffold A, highly variable B, which determines substrate specificity holoenzymes. Mutant leaves knockdown pp2a-b′γ plants show disintegration chloroplasts premature...
Western-style diets (WD) high in fat and scarce fiber vitamin D increase risks of colorectal cancer. Here, we performed a long-term diet study mice to follow tumorigenesis characterize structural metabolic changes colon mucosa associated with WD predisposition increased tumor numbers, proteomic analysis indicated severe deregulation intracellular bile acid (BA) homeostasis activation cell proliferation. also crypt depth Despite luminal BA, colonocytes from WD-fed exhibited decreased...
Summary Wounding results in the controlled cell death of a few rows cells adjacent to disrupted resulting physical wound closure, which combined with phenolic compound deposition, prevents water loss and pathogen entry. The control these processes remains uncharacterized. Cell mutant A rabidopsis thaliana lacking BOTRYTIS SENSITIVE 1 / MYB 108 ( BOS ) function was characterized utilizing physiological, biological genetic methods. bos1 has induced runaway that includes enhanced reactive...
Cuticular defects trigger a battery of reactions including enhanced reactive oxygen species (ROS) production and resistance to necrotrophic pathogens. However, the source ROS generated by such impaired cuticles has remained elusive. Here, we report characterization Arabidopsis thaliana ohy1 mutant, Peroxidase 57 (PER57) - overexpressing line that demonstrates defense responses result both from increased accumulation permeability leaf cuticle. The mutant was identified in screen A. seedlings...
Studies have uncovered delicate mechanisms that enable plant acclimation to fluctuating light. Translating the knowledge controlled environment agriculture could advance development of cost-effective dynamic lighting strategies, but effects varying light intensities on vegetable crops remain poorly understood. Here we recorded chlorophyll fluorescence, photosynthetic activity, metabolic responses, and growth lettuce (Lactuca sativa L.) cv. Katusa under lighting. The intensity was varied at...
Rcd1 (radical-induced cell death1) is an Arabidopsis thaliana mutant, which exhibits high tolerance to paraquat [methyl viologen (MV)], herbicide that interrupts photosynthetic electron transport chain causing the formation of superoxide and inhibiting NADPH production in chloroplast. To understand biochemical mechanisms MV-resistance role RCD1 oxidative stress responses, we performed metabolite profiling wild type (Col-0) rcd1 plants light, after MV exposure prolonged darkness. The function...
A series of novel 5-aminothiazole-based ligands for prolyl oligopeptidase (PREP) comprise selective, potent modulators the protein–protein interaction (PPI)-mediated functions PREP, although they are only weak inhibitors proteolytic activity PREP. The disconnected structure–activity relationships significantly more pronounced than earlier published 5-aminooxazole-based ligands. Furthermore, stability 5-aminothiazole scaffold allowed exploration wider substitution patterns that was possible...
Mixed virus infections threaten crop production because interactions between the host and pathogen mix may lead to viral synergism. While individual by potato A (PVA), a potyvirus, X (PVX), potexvirus, can be mild, co-infection leads synergistic enhancement of PVX severe symptoms. We combined image-based phenotyping with metabolite analysis single mixed PVA compared their effects on growth, photosynthesis, metabolites in Nicotiana benthamiana. Viral synergism was evident symptom severity...
Abstract Accumulating evidence suggests mitochondria as key modulators of normal and premature aging, yet whether primary oxidative phosphorylation (OXPHOS) deficiency can cause progeroid disease remains unclear. Here, we show that mice with severe isolated respiratory complex III (CIII) display nuclear DNA damage, cell cycle arrest, aberrant mitoses, cellular senescence in the affected organs such liver kidney, a systemic phenotype resembling juvenile-onset syndromes. Mechanistically, CIII...
Stored potato (Solanum tuberosum L.) tubers are sensitive to wet conditions that can cause rotting in long-term storage. To study the effect of water on tuber surface during storage, microarray analysis, RNA-Seq profiling, qRT-PCR and phytohormone measurements were performed gene expression hormone content incubated at two temperatures: 4 °C 15 °C. The growth plants was also observed a greenhouse after incubation conditions. Wet induced low-oxygen response, suggesting reduced oxygen...
Post-transcriptional RNA modifications play an important role in cellular metabolism with homoeostatic disturbances manifesting as a wide repertoire of phenotypes, reduced stress tolerance and translational perturbation, developmental defects, diseases, such type II diabetes, leukaemia, carcinomas. Hence, there has been intense effort to develop various methods for investigating their roles organisms, including sequencing-based approaches and, more frequently, liquid chromatography–mass...
In this study, we show that infrared laser ablation atmospheric pressure photoionization mass spectrometry (LAAPPI-MS) imaging with 70 μm lateral resolution allows for the analysis of Arabidopsis thaliana (A. thaliana) leaf substructures ranging from single-cell trichomes and interveinal lamina to primary, secondary, tertiary veins. The method also showed its potential depth profiling first time by mapping analytes at different depths spatially resolving topmost cuticular wax layer...