A5016072545- Alkaloids: synthesis and pharmacology
- Plant tissue culture and regeneration
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Microbial Natural Products and Biosynthesis
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
- Plant Virus Research Studies
- Natural product bioactivities and synthesis
- Biochemical and biochemical processes
- Berberine and alkaloids research
- Horticultural and Viticultural Research
- Fungal and yeast genetics research
- Plant Molecular Biology Research
- Phytochemical Studies and Bioactivities
- Polyamine Metabolism and Applications
- Fermentation and Sensory Analysis
- Traditional and Medicinal Uses of Annonaceae
- Plant-Microbe Interactions and Immunity
- Bioinformatics and Genomic Networks
- Sirtuins and Resveratrol in Medicine
- Gene expression and cancer classification
- Plant and Fungal Species Descriptions
- Coffee research and impacts
- Peroxisome Proliferator-Activated Receptors
Université de Tours
2016-2025
Laboratoire des Biomolécules
2013-2022
Alzahra University
2022
University of Helsinki
2012
Institut de Biologie Moléculaire des Plantes
2005-2008
Biocenter Finland
2008
Centre National de la Recherche Scientifique
2005
In Arabidopsis thaliana, silencing of hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT), a lignin biosynthetic gene, results in strong reduction plant growth. We show that, HCT-silenced plants, synthesis repression leads to the redirection metabolic flux into flavonoids through chalcone synthase activity. Several flavonol glycosides and acylated anthocyanin were shown accumulate higher amounts silenced plants. By contrast, sinapoylmalate levels barely affected,...
The plant-specific WRKY transcription factor (TF) family with 74 members in Arabidopsis thaliana appears to be involved the regulation of various physiological processes including plant defence and senescence. WRKY53 WRKY70 were previously implicated as positive negative regulators senescence, respectively. Here putative function other group III proteins leaf senescence has been explored results suggest involvement two additional TFs, 54 WRKY30, this process. structurally related WRKY54...
The Madagascar periwinkle, Catharanthus roseus, belongs to the Apocynaceae family.This medicinal plant, endemic Madagascar, produces many important drugs including monoterpene indole alkaloids (MIA) vincristine and vinblastine used treat cancer worldwide.Here, we provide a new version of C. roseus genome sequence obtained through combination Oxford Nanopore Technologies long-reads Illumina short-reads.This more contiguous assembly consists 173 scaffolds with total length 581.128Mb an N50...
Summary WRKY transcription factors ( TF s) have been mainly associated with plant defense, but recent studies suggested additional roles in the regulation of other physiological processes. Here, we explored possible contribution two related group III s, 70 and 54, to osmotic stress tolerance. These s are positive regulators co‐operate as negative salicylic acid SA ) biosynthesis senescence. We employed single double mutants wrky54 wrky70 , well a WRKY70 overexpressor line, explore role these...
Summary BAHD acyltransferases catalyze the acylation of many plant secondary metabolites. We characterized function At2g19070 , a member gene family Arabidopsis thaliana . The acyltransferase was shown to be specifically expressed in anther tapetum cells early stages flower development. impact repression studied RNAi plants and knockout (KO) mutant line. Immunoblotting with specific antiserum raised against recombinant protein used evaluate accumulation product flowers various genotypes...
Abstract Hydroxylation of tabersonine at the C-16 position, catalyzed by 16-hydroxylase (T16H), initiates synthesis vindoline that constitutes main alkaloid accumulated in leaves Catharanthus roseus. Over last decade, this reaction has been associated with CYP71D12 cloned from undifferentiated C. roseus cells. In study, we isolated a second cytochrome P450 (CYP71D351) displaying T16H activity. Biochemical characterization demonstrated and CYP71D351 both exhibit high affinity for narrow...
Abstract Monoterpenoid indole alkaloids (MIAs) represent a large class of plant natural products with marketed pharmaceutical activities against wide range indications, including cancer, malaria and hypertension. Halogenated MIAs have shown improved properties; however, synthesis new-to-nature halogenated remains challenge. Here we demonstrate platform for de novo biosynthesis two MIAs, serpentine alstonine, in baker’s yeast Saccharomyces cerevisiae deploy it to systematically explore the...
trans-Resveratrol (1a) is a phytoalexin produced by plants in response to infections pathogens. Its potential activity against clinically relevant opportunistic fungal pathogens has previously been poorly investigated. Evaluated herein are the candidacidal activities of oligomers (2a, 3–5) 1a purified from Vitis vinifera grape canes and several analogues (1b–1j) obtained through semisynthesis using methylation acetylation. Moreover, trans-ε-viniferin (2a), dimer 1a, was also subjected (2b)...
Co-expression networks are essential tools to infer biological associations between gene products and predict annotation. Global can be analyzed at the transcriptome-wide scale or after querying them with a set of guide genes capture transcriptional landscape given pathway in process named Pathway Level Coexpression (PLC). A critical step network construction remains definition co-expression. In present work, we compared how Pearson Correlation Coefficient (PCC), Spearman (SCC), their...
Abstract Phenolamides represent a family of specialized metabolites, consisting the association hydroxycinnamic acid derivatives with aliphatic or aromatic amines. Since discovery first phenolamide in late 1940s, decades phytochemical analyses have revealed high structural diversity for this and wide distribution plant kingdom. The occurrence structurally diverse phenolamides almost all organs has led to early hypotheses on their involvement floral initiation fertility, as well defense...
Abstract Monoterpene indole alkaloids (MIAs) are a structurally diverse family of specialized metabolites mainly produced in Gentianales to cope with environmental challenges. Due their pharmacological properties, the biosynthetic modalities several MIA types have been elucidated but not that yohimbanes. Here, we combine metabolomics, proteomics, transcriptomics and genome sequencing Rauvolfia tetraphylla machine learning discover unexpected multiple actors this natural product synthesis. We...
Natural products represent an inestimable source of valuable compounds for human health. Notably, those produced by plants remain challenging to access due their low production. Potential shortages plant-derived biopharmaceuticals caused climate change or pandemics also regularly tense the market trends. Thus, biotechnological alternatives supply based on synthetic biology have emerged. These innovative strategies mostly rely use engineered microbial systems compound synthesis. In this...
Grape canes are vineyard waste products containing valuable phytochemicals of medicine and agriculture interest. storage is critical for the accumulation these bioactive compounds. In present study, we investigated changes in stilbenoid phytochemical composition during grape cane influence temperature on final concentrations. A strong increase concentration monomer E-resveratrol (approximately 40-fold) was observed first 6 weeks at 20 °C eight different varieties without any change oligomer...
Transcriptome sequencing offers a great resource for the study of non-model plants such as Catharanthus roseus, which produces valuable monoterpenoid indole alkaloids (MIAs) via complex biosynthetic pathway whose characterization is still undergoing. databases dedicated to this plant were recently developed by several consortia uncover new genes. However, identification missing steps in MIA biosynthesis based on these large datasets may be limited erroneous assembly close transcripts and...
Phenolamides, so called hydroxycinnamic acid amides, are specialized metabolites produced in higher plants, involved development, reproduction and serve as defence compounds biotic interactions. Among them, trihydroxycinnamoyl spermidine derivatives were initially found to be synthetized by a hydroxycinnamoyltransferase (AtSHT) Arabidopsis thaliana accumulate the pollen coat. This study reports identification, Malus domestica, of an acyltransferase able complement sht mutant Arabidopsis. The...
Summary While the characterization of biosynthetic pathway monoterpene indole alkaloids ( MIA s) in leaves Catharanthus roseus is now reaching completion, only two enzymes from root counterpart dedicated to tabersonine metabolism have been identified date, namely 19‐hydroxylase (T19H) and minovincine 19‐ O ‐acetyltransferase MAT ). Albeit recombinant catalyzes acetylation at low efficiency vitro , we demonstrated that was inactive when expressed yeast planta suggesting an alternative...
Abstract Plants deploy distinct secondary metabolisms to cope with environment pressure and face bio-aggressors notably through the production of biologically active alkaloids. This metabolism-type is particularly elaborated in Catharanthus roseus that synthesizes more than a hundred different monoterpene indole alkaloids (MIAs). While characterization their biosynthetic pathway now reaches completion, still little known about role MIAs during biotic attacks. As consequence, we developed new...
Many of the plant-derived compounds used in chemotherapies are currently produced by semisynthesis, which results limited supplies at exorbitant market prices. However, synthetic biology era, began ca 15 years ago, has progressively yielded encouraging advances using engineered microbes for practical production cheaper plant anticancer drugs. Plants represent a seemingly inexhaustible source biologically active compounds. Some these have been essential components therapeutic arsenal many...
OPINION article Front. Fungal Biol., 22 September 2021 | https://doi.org/10.3389/ffunb.2021.733492
ABSTRACT Monoterpene indole alkaloids (MIAs) constitute a vast group of plant natural products synthesized within the Gentianales order. MIAs possess outstanding pharmacological properties that explain their wide use in treatment human diseases. These biological activities result from complex structure originate intricated biosynthetic pathways involving several enzyme families and notably cytochrome P450s. The early steps MIA synthesis involve P450s 71 clade, which catalyse conversion...
Expansion of the biosynthesis plant specialized metabolites notably results from massive recruitment cytochrome P450s that catalyze multiple types conversion biosynthetic intermediates. For catalysis, require a two-electron transfer catalyzed by shared P450 oxidoreductases (CPRs), making these auxiliary proteins an essential component metabolism. CPR isoforms usually group into two distinct classes with different proposed roles, namely involvement in primary and basal metabolisms for class I...
Lochnericine is a major monoterpene indole alkaloid (MIA) in the roots of Madagascar periwinkle (Catharanthus roseus). derived from stereoselective C6,C7-epoxidation tabersonine and can be metabolized further to generate other complex MIAs. While enzymes responsible for its downstream modifications have been characterized, those involved lochnericine biosynthesis remain unknown. By combining gene correlation studies, functional assays, transient inactivation, we identified two highly...
Grape accumulates numerous polyphenols with abundant health benefit and organoleptic properties that