A5083724241- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Phytochemicals and Antioxidant Activities
- Protein Hydrolysis and Bioactive Peptides
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Biochemical and biochemical processes
- Sirtuins and Resveratrol in Medicine
- Probiotics and Fermented Foods
- Metabolomics and Mass Spectrometry Studies
- Alkaloids: synthesis and pharmacology
- Plant Gene Expression Analysis
- Salivary Gland Disorders and Functions
- Biochemical effects in animals
- Botulinum Toxin and Related Neurological Disorders
- Bone Tumor Diagnosis and Treatments
- Ginseng Biological Effects and Applications
- Phytoestrogen effects and research
- Meningioma and schwannoma management
- Berberine and alkaloids research
- Epilepsy research and treatment
- Plant-derived Lignans Synthesis and Bioactivity
- Soft tissue tumor case studies
- Plant responses to water stress
- Coffee research and impacts
Procédés Alimentaires et Microbiologiques
2022-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023-2024
Université de Tours
2017-2024
Biopolymères Interactions Assemblages
2023-2024
Centre Hospitalier Universitaire de La Réunion
2024
Université de Bourgogne
2023
Novartis (France)
2019
Ecology and Ecosystem Health
2018
Institut Agro Rennes-Angers
2018
Summary While the characterization of biosynthetic pathway monoterpene indole alkaloids ( MIA s) in leaves Catharanthus roseus is now reaching completion, only two enzymes from root counterpart dedicated to tabersonine metabolism have been identified date, namely 19‐hydroxylase (T19H) and minovincine 19‐ O ‐acetyltransferase MAT ). Albeit recombinant catalyzes acetylation at low efficiency vitro , we demonstrated that was inactive when expressed yeast planta suggesting an alternative...
Grape canes are waste biomass of viticulture containing bioactive polyphenols valuable in cosmetics. Whereas several studies reported the cosmetic activities E-resveratrol, only few described potential E-ε-viniferin, second major constituent grape cane extracts (GCE), and none them investigated GCE as a natural blend for applications. In this study, we considered from polyphenol-rich varieties multifunctional ingredients. HPLC analysis was performed to quantify i.e., catechin, epicatechin,...
Microbiological, chemical, and sensory analyses were coupled to understand the origins of kombucha organoleptic compounds their implication in flavor beverage. By isolating microorganisms from an original comparing it monocultures cocultures two yeasts (Brettanomyces bruxellensis Hanseniaspora valbyensis) acetic acid bacterium (Acetobacter indonesiensis), interaction effects investigated during phases production. 32 volatile identified quantified by Headspace-Solid Phase-MicroExtraction-Gas...
Lochnericine is a major monoterpene indole alkaloid (MIA) in the roots of Madagascar periwinkle (Catharanthus roseus). derived from stereoselective C6,C7-epoxidation tabersonine and can be metabolized further to generate other complex MIAs. While enzymes responsible for its downstream modifications have been characterized, those involved lochnericine biosynthesis remain unknown. By combining gene correlation studies, functional assays, transient inactivation, we identified two highly...
Grape accumulates numerous polyphenols with abundant health benefit and organoleptic properties that
Beneficial polyphenols in apples can reach the stomach as complexes formed with salivary proteins. The present study aimed at documenting interactions between proteins and cider apple fate of during gastric digestion. A polyphenolic extract was mixed human saliva, were characterized by analyzing insoluble soluble fractions mixtures, before after vitro Results confirmed that proline-rich efficiently precipitate suggested two zinc-binding also form polyphenols. classes involved such depended...
Plasmopara viticola control in organic viticulture requires copper-based fungicides with harmful effects on health and the environment. Plant extracts represent a biorational eco-friendly alternative to copper. The aim of this study was evaluate potential stilbenoid-rich grape cane extract (GCE) against downy mildew three cultivars over 3 years following natural infection.Over all field trials, GCE treatments showed an average reduction disease incidence -35% -38% leaves clusters,...
Grape downy mildew is a devastating disease worldwide and new molecular phenotyping tools are required to detect metabolic changes associated plant symptoms. In this purpose, we used UPLC-DAD-MS-based semi-targeted metabolomics screen symptomatic leaves that expressed oil spots (6 dpi, days post-infection) necrotic lesions (15 dpi) under natural infections in the field. Leaf extract analyses enabled identification of 47 metabolites belonging primary metabolism including 6 amino acids 1...
Grape canes represent a valuable source of numerous polyphenols with antioxidant properties, whose compositions vary depending on the genotype and environmental factors. Antioxidant activities pure molecules are often reported without considering possible interactions that may occur in complex polyphenol mixture. Using UPLC-MS-based metabolomics unsupervised classification, we explored variations grape cane extracts from collection European varieties. were assessed using ORAC, ABTS, DPPH,...
The composition of bioactive polyphenols from grape canes, an important viticultural byproduct, was shown to be varietal-dependent; however, the influence soil-related terroir factors remains unexplored. Using spatial metabolomics and correlation-based networks, we investigated how continuous changes in soil features topography may impact polyphenol canes. Soil properties, topography, cane extracts were analyzed at georeferenced points over 3 consecutive years, followed by UPLC-DAD-MS-based...
Abstract In the context of expansion invasive species, survival plants is conditioned by their ability to adapt. France, water primrose Ludwigia grandiflora , an aquatic invades yet wet meadows, leading a depreciation fodder value. Understanding its potential adaption necessary management, strong differences between both morphotypes were expected. So morphological and metabolic responses terrestrial environment analyzed for morphotypes. All biomass variables greater in morphotype than...
The application of plant tissue culture in cosmetics is gaining a new impetus the last few decades and it anticipated that cosmetic industries coming years will rise to an annual turnover hundred billion US dollars. Here, we focused on Linum usitatissimum (L.) well-known for its potent properties. Following their establishments from 3 distinct initial explant origins (root, hypocotyl cotyledon) selection optimal hormonal concentrations, 2 vitro systems (callus vs cell suspensions) were...
Grape seed residues represent the raw material to produce several value-added products including polyphenol-rich extracts with nutritional and health attributes. Although impact of variety environmental conditions on polyphenol composition in fresh berries is recognized, no data are available regarding grape residues. The chemical from wine distilleries France, Spain Italy was characterized by mass spectrometry. Forty-two metabolites were identified belonging non-galloylated galloylated...
Grape canes are viticulture byproducts representing a sustainable source of valuable bioactive polyphenols. However, varietal origin and postharvest treatment greatly influence the effective concentration biomolecules, thus limiting industrial development. With aim to develop grape cane extracts with high polyphenol contents, selection specific variety combined optimized is major determinant. A previously described comprising cutting grapevine stalks 0.5 cm length storage at 15–20 °C over 2...
After bud burst, a transcriptional reprogramming of the shikimate and phenylpropanoid pathways occurs in grapevine canes resulting accumulation stilbenoids like resveratrol viniferin. Stilbenoids are compounds with important biological properties biotechnological applications that synthesized response to different stresses. Although they found woody tissues, such as buds, their biosynthesis have been essentially described berries. We previously shown transcripts encoding secondary metabolism...
The composition of the juice from grape berries is at basis definition technological ripeness before harvest, historically evaluated global sugar and acid contents. If many studies have contributed to identification other primary secondary metabolites in whole berries, deepening knowledge about chemical sole flesh (i.e., without considering skins seeds) harvest interest when studying enological potential widespread varieties producing high-added-value wines. Here, we used non-targeted...