A5084663042- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant biochemistry and biosynthesis
- Horticultural and Viticultural Research
- Plant Virus Research Studies
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Plant Gene Expression Analysis
- Fermentation and Sensory Analysis
- Microbial Metabolic Engineering and Bioproduction
- Natural product bioactivities and synthesis
- Photosynthetic Processes and Mechanisms
- Polyamine Metabolism and Applications
- Metabolomics and Mass Spectrometry Studies
- Fungal Biology and Applications
- Insect and Pesticide Research
- Antioxidant Activity and Oxidative Stress
- Antifungal resistance and susceptibility
- Alkaloids: synthesis and pharmacology
- Ubiquitin and proteasome pathways
- Transgenic Plants and Applications
- Cholinesterase and Neurodegenerative Diseases
Université de Tours
2010-2023
Laboratoire des Biomolécules
2022
Institut National de la Recherche Agronomique
2013
Centre National de la Recherche Scientifique
2006-2008
Environmental Molecular Sciences Laboratory
2008
Institut Jean-Pierre Bourgin
2008
Centre National de Recherche en Génomique Humaine
2006
Laboratoire Physiologie Cellulaire & Végétale
2002
Université Clermont Auvergne
1996
Abstract Nitrate releases seed dormancy in Arabidopsis (Arabidopsis thaliana) Columbia accession seeds part by reducing abscisic acid (ABA) levels. led to lower levels of ABA imbibed when included the germination medium (exogenous nitrate). also reduced dry provided mother plant during development (endogenous Transcript profiling treated with or without nitrate revealed that exogenous a higher expression nitrate-responsive genes, whereas endogenous profile similar stratified after-ripened...
Abstract The molecular mechanisms underlying plant cell totipotency are largely unknown. Here, we present a protocol for the efficient regeneration of plants from Arabidopsis thaliana protoplasts. specific liquid medium used in our study leads to high rate reentry into cycle most types, providing powerful system dedifferentiation/regeneration processes independent somatic cells. To identify early events establishment totipotency, monitored genome-wide transcript profiles plantlets and...
Abstract NUCLEOSOME ASSEMBLY PROTEIN1 (NAP1) is conserved from yeast to human and was proposed act as a histone chaperone. While budding contains single NAP1 gene, multicellular organisms, including plants animals, contain several NAP1-RELATED PROTEIN (NRP) genes. However, the biological role of these genes has been largely unexamined. Here, we show that, in Arabidopsis thaliana, simultaneous knockout two NRP genes, NRP1 NRP2, impaired postembryonic root growth. In nrp1-1 nrp2-1 double...
Co-expression networks are essential tools to infer biological associations between gene products and predict annotation. Global can be analyzed at the transcriptome-wide scale or after querying them with a set of guide genes capture transcriptional landscape given pathway in process named Pathway Level Coexpression (PLC). A critical step network construction remains definition co-expression. In present work, we compared how Pearson Correlation Coefficient (PCC), Spearman (SCC), their...
ATM (Ataxia Telangiectasia Mutated) is an essential checkpoint kinase that signals DNA double-strand breaks in eukaryotes. Its depletion causes meiotic and somatic defects Arabidopsis progressive motor impairment accompanied by several cell deficiencies patients with ataxia telangiectasia (AT). To obtain a comprehensive view of the pathway plants, we performed time-course analysis seedling responses combining confocal laser scanning microscopy studies root development genome-wide expression...
Phenolamides, so called hydroxycinnamic acid amides, are specialized metabolites produced in higher plants, involved development, reproduction and serve as defence compounds biotic interactions. Among them, trihydroxycinnamoyl spermidine derivatives were initially found to be synthetized by a hydroxycinnamoyltransferase (AtSHT) Arabidopsis thaliana accumulate the pollen coat. This study reports identification, Malus domestica, of an acyltransferase able complement sht mutant Arabidopsis. The...
Abstract Plants deploy distinct secondary metabolisms to cope with environment pressure and face bio-aggressors notably through the production of biologically active alkaloids. This metabolism-type is particularly elaborated in Catharanthus roseus that synthesizes more than a hundred different monoterpene indole alkaloids (MIAs). While characterization their biosynthetic pathway now reaches completion, still little known about role MIAs during biotic attacks. As consequence, we developed new...
In higher plants, PII is a nuclear-encoded plastid protein which homologous to bacterial signalling proteins known be involved in the regulation of nitrogen metabolism. A reduced ornithine, citrulline and arginine accumulation was observed two Arabidopsis knock-out mutants response NH4+ resupply after N starvation. This difference could explained by key enzyme biosynthesis pathway, N-acetyl glutamate kinase (NAGK) PII. vitro assays using purified recombinant showed catalytic activation NAGK...
Abstract Large-scale gene co-expression networks are an effective methodology to analyze sets of co-expressed genes and discover new functions or associations. Distances between estimated according their expression profiles visualized in that may be further partitioned reveal communities genes. Creating is now eased by the large amounts publicly available data (microarrays RNA-seq). Although many distance calculation methods have been intensively compared reviewed past, it unclear how...
Cytokinin signaling is a key regulatory pathway of many aspects in plant development and environmental stresses. Herein, we initiated the identification functional characterization five CHASE-containing Histidine Kinases (CHK) economically important Malus domestica species. These cytokinin receptors named MdCHK2, MdCHK3a/MdCHK3b MdCHK4a/MdCHK4b by homology with Arabidopsis AHK clearly displayed three distinct profiles. The groups exhibited architectural variations, especially N-terminal part...
The composition of bioactive polyphenols from grape canes, an important viticultural byproduct, was shown to be varietal-dependent; however, the influence soil-related terroir factors remains unexplored. Using spatial metabolomics and correlation-based networks, we investigated how continuous changes in soil features topography may impact polyphenol canes. Soil properties, topography, cane extracts were analyzed at georeferenced points over 3 consecutive years, followed by UPLC-DAD-MS-based...
Calcium-mediated signalling is ubiquitous in both animals and plants. Changes cytoplasmic free Ca2+ concentration couple diverse arrays of stimuli to their specific responses, the specificity stimulus being determined by integrated actions between multiple mobilization pathways. In this work, a pharmacological approach reported, aimed at deciphering role calcium as second messenger transduction pathway leading inhibitory effect 2,4-dichlorophenoxyacetic acid (2,4-D), regulating monoterpene...
Young tomato plants (Lycopersicon esculentum, 8 days old) were given a heat-wound to cotyledon. The resulting electrical activity at the hypocotyl level was monitored with intracellular microelectrodes. We observed an original pattern of slow wave potentials (SWPs), consisting 2-3 waves, associated spikes. electrophysiological study SWPs confirms previous conclusions that are due inhibition active component membrane potential. spikes shows they fit particularities putative action (APs). They...
Involvement of Ca(2+) signalling in regulation the biosynthesis monoterpene indole alkaloids (MIA) Catharanthus roseus has been extensively studied recent years, albeit no protein this pathway isolated. Using a PCR strategy, two C. cDNAs encoding distinct calmodulin (CAM) isoforms were cloned and named CAM1 CAM2. The deduced 149 amino acid sequences possess four binding domains exhibit close identity with Arabidopsis CAM (>91%). ability CAM2 to bind was demonstrated following expression...
Abstract Domain of unknown function 239 (DUF239) is a conserved sequence found in the catalytic site Neprosins which are specific secreted prolyl endopeptidases Nepenthes genus. participate nitrogen cycle by digesting preys trapped pitcher these carnivorous plants. Apart from that, DUF239s have been poorly documented We identified 50 genes containing DUF239‐coding sequences Arabidopsis genome that distributed across six distinct phylogenetic clusters. The chromosomal distribution suggests...