A5040322382- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Photoreceptor and optogenetics research
- Mitochondrial Function and Pathology
- Spectroscopy and Quantum Chemical Studies
- Wittgensteinian philosophy and applications
- Enzyme Structure and Function
- Light effects on plants
- Plant Molecular Biology Research
- Plant responses to elevated CO2
- Amino Acid Enzymes and Metabolism
- Lipid metabolism and biosynthesis
- Epistemology, Ethics, and Metaphysics
- Coastal wetland ecosystem dynamics
- Pragmatism in Philosophy and Education
- Metabolism and Genetic Disorders
- Plant Water Relations and Carbon Dynamics
- Algal biology and biofuel production
- ATP Synthase and ATPases Research
- American Constitutional Law and Politics
- Plant Gene Expression Analysis
- Coastal and Marine Dynamics
- Philosophy, Ethics, and Existentialism
- GABA and Rice Research
Vanderbilt University
1979-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Centre National de la Recherche Scientifique
2013-2024
Université Paris Cité
2010-2024
Université Paris-Saclay
1987-2024
South Carolina Department of Natural Resources
2009-2023
Institut des Sciences des Plantes de Paris Saclay
2012-2022
Bronx Community College
2022
Université Paris-Sud
2010-2020
Core Laboratories (United States)
2018
Abstract While the possible importance of tricarboxylic acid (TCA) cycle reactions for leaf photosynthesis operation has been recognized, many uncertainties remain on whether TCA biochemistry is similar in light compared with dark. It widely accepted that day respiration and metabolic commitment to decarboxylation are down-regulated illuminated leaves. However, basis (i.e. limiting steps involved such a down-regulation) not well known. Here, we investigated vivo fluxes individual by...
Day respiration is the process by which nonphotorespiratory CO2 produced illuminated leaves. The biological function of day respiratory metabolism a major conundrum plant photosynthesis research: because rate evolution partly inhibited in light, it viewed as either detrimental to carbon balance or necessary for operation (e.g., providing cytoplasmic ATP sucrose synthesis). Systematic variations under contrasting environmental conditions have been used elucidate metabolic rationale light....
The PII protein is a signal integrator involved in the regulation of nitrogen metabolism bacteria and plants. Upon sensing cellular carbon energy availability, conveys by interacting with target proteins, thereby modulating their biological activity. Plant located to plastids; therefore, identify new PII-affinity chromatography soluble extracts from Arabidopsis leaf chloroplasts was performed. Several proteins were retained only when Mg-ATP present binding medium they specifically released...
• Nitrogen assimilation in leaves requires primary NH2 acceptors that, turn, originate from carbon metabolism. Respiratory metabolism is believed to provide such (such as 2-oxoglutarate), so that day respiration commonly seen a cornerstone for nitrogen into glutamate illuminated leaves. However, both glycolysis and respiratory CO2 evolution are known be inhibited by light, thereby compromising the input of recent photosynthetic production. In this study, we carried out isotopic labelling...
Abstract Photosynthesis—the conversion of energy from sunlight into chemical energy—is essential for life on Earth. Yet there is much we do not understand about photosynthetic a fundamental level: how it evolved and the extent its diversity, dynamics, all components connections involved in regulation. In this commentary, researchers working aspects photosynthesis including light-dependent reactions, photorespiration, C4 metabolism pose discuss what they view as most compelling open questions...
A cDNA from <i>Arabidopsis thaliana</i>and four related cDNAs <i>Nicotiana tabacum</i> that we have isolated encode hitherto unidentified members of the mitochondrial carrier family. These proteins been overexpressed in bacteria and reconstituted into phospholipid vesicles. Their transport properties demonstrate they are orthologs/isoforms a novel capable transporting both dicarboxylates (such as malate, oxaloacetate, oxoglutarate, maleate) tricarboxylates citrate, isocitrate,...
Screening of the Arabidopsis thaliana genome revealed three potential homologues mammalian and yeast mitochondrial DICs (dicarboxylate carriers) designated as DIC1, DIC2 DIC3, each belonging to carrier protein family. DIC1 are broadly expressed at comparable levels in all tissues investigated. DIC1–DIC3 have been reported previously uncoupling proteins, but direct transport assays with recombinant reconstituted DIC proteins clearly demonstrate that their substrate specificity is unique...
Cytosolic NADP-dependent isocitrate dehydrogenase (cICDH) produces 2-oxoglutarate (2-OG) and NADPH, is encoded by a single gene in Arabidopsis thaliana. Three allelic lines carrying T-DNA insertions this showed less than 10% extractable leaf ICDH activity, but only relatively small decreases growth compared to wild-type Col0. Metabolite profiling gas chromatography–time of flight–mass spectrometry (GC–TOF–MS) high-performance liquid chromatography (HPLC) revealed that loss cICDH function...
Intense efforts are currently devoted to elucidate the metabolic networks of plants, in which nitrogen assimilation is particular importance because it strongly related plant growth. In addition, at leaf level, primary metabolism interacts with photosynthesis, day respiration, and photorespiration, simply needs energy, reductant, carbon skeletons provided by these processes. While some recent studies have focused on metabolomics genomics leaves, actual fluxes associated operating leaves not...
NAD-dependent isocitrate dehydrogenase (IDH) is a tricarboxylic acid cycle enzyme that produces 2-oxoglutarate, an organic required by the glutamine synthetase/glutamate synthase to assimilate ammonium. Three Arabidopsis (Arabidopsis thaliana) IDH mutants have been characterized, corresponding insertion into different gene (At5g03290, idhv; At4g35260, idhi; At2g17130, idhii). Analysis of mRNA and protein show each mutant lacks products. Leaf activity reduced 92%, 60%, 43% for idhv, idhi,...
Transgenic tomato (Solanum lycopersicum) plants were generated expressing a fragment of the mitochondrial NAD-dependent isocitrate dehydrogenase gene (SlIDH1) in antisense orientation. The transgenic displayed mild reduction activity target enzyme leaves but essentially no visible alteration growth from wild-type. Fruit size and yield were, however, reduced. These characterized by relatively few changes photosynthetic parameters, they minor decrease maximum efficiency (Fv/Fm). Furthermore,...
Arabidopsis thaliana sucrose nonfermenting 1-related protein kinase 1 complexes belong to the SNF1/AMPK/SnRK1 family that shares an ancestral function as central regulators of metabolism. In A. thaliana, products AtSnAK1 and AtSnAK2, orthologous yeast genes, have been shown autophosphorylate phosphorylate/activate AtSnRK1.1 catalytic subunit on Thr(175). The phosphorylation these kinases has investigated by site-directed mutagenesis tandem mass spectrometry. autophosphorylation site AtSnAK2...
Photosynthetic CO2 assimilation is the carbon source for plant anabolism, including amino acid production and protein synthesis. The biosynthesis of leaf proteins known decades to correlate with photosynthetic activity but mechanisms controlling this effect are not documented. cornerstone regulation synthesis believed be translation initiation, which involves multiple phosphorylation events in Eukaryotes. We took advantage phosphoproteomic methods applied Arabidopsis thaliana rosettes...