A5013216979- Gut microbiota and health
- Mycorrhizal Fungi and Plant Interactions
- Environmental DNA in Biodiversity Studies
- Identification and Quantification in Food
- Plant Pathogens and Fungal Diseases
- Endoplasmic Reticulum Stress and Disease
- Plant tissue culture and regeneration
- Cellular transport and secretion
- Forest Ecology and Biodiversity Studies
- Species Distribution and Climate Change
- Berry genetics and cultivation research
- Plant Stress Responses and Tolerance
- Polyamine Metabolism and Applications
- Plant Molecular Biology Research
- Horticultural and Viticultural Research
- Phytochemicals and Antioxidant Activities
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Inflammasome and immune disorders
- Erythrocyte Function and Pathophysiology
- Hereditary Neurological Disorders
- Cassava research and cyanide
- Infant Nutrition and Health
- Renewable Energy and Sustainability
- Turfgrass Adaptation and Management
University of Oulu
2013-2024
Abstract Diets play a key role in understanding trophic interactions. Knowing the actual structure of food webs contributes greatly to our biodiversity and ecosystem functioning. The research prey preferences different predators requires knowledge not only consumed, but also what is available. In this study, we applied DNA metabarcoding analyze diet 4 bird species (willow tits Poecile montanus , Siberian cinctus great Parus major blue Cyanistes caeruleus ) by using feces nestlings....
Reports regarding the presence of bacteria in fetal environment remain limited and controversial. Recently, extracellular vesicles secreted by human gut microbiota have emerged as a novel mechanism for host-microbiota interaction. We aimed to investigate bacterial during healthy pregnancies determine whether derived from can cross biological barriers reach fetus.Bacterial were detectable amniotic fluid pregnant women, exhibiting similarities found maternal microbiota. In mice, intra-amniotic...
Gut microbiome–derived nanoparticles, known as bacterial extracellular vesicles (bEVs), have garnered interest promising tools for studying the link between gut microbiome and human health. The diverse composition of bEVs, including their proteins, mRNAs, metabolites, lipids, makes them useful investigating diseases such cancer. However, conventional approaches alone may not be accurate in deciphering host–gut communication. In clinical research, there is a gap knowledge on role bEVs solid...
Polyamines are small metabolites present in all living cells and play fundamental roles numerous physiological events plants. The aminopropyltransferases (APTs), spermidine synthase (SPDS), spermine (SPMS) thermospermine (ACL5), essential enzymes the polyamine biosynthesis pathway. In angiosperms, SPMS has evolved from SPDS via gene duplication, whereas gymnosperms APTs mostly unexplored no been reported. study aimed to investigate functional properties of ACL5 proteins Scots pine (Pinus...
Aberrant secretion of lysosomal hydrolases such as (pro)cathepsin D (proCD) is a common phenotypic change in many human cancers. Here we explore the underlying molecular defect(s) and find that MCF-7 breast CaCo-2 colorectal cancer cells are unable to acidify their endosomal compartments secreted higher amounts proCD than did acidification-competent cell types. The latter equivalent only after dissipation organellar pH gradients with NH(4)Cl. Assessing critical steps resulted revealed...
The northern regions are experiencing considerable changes in winter climate leading to more frequent warm periods, rain-on-snow events and reduced snow pack diminishing the insulation properties of cover increasing soil frost freeze-thaw cycles. In this study, we investigated how lack cover, formation ice encasement compaction affect size, structure activities bacterial fungal communities. Contrary our hypotheses, manipulation treatments over one had limited influence on microbial community...
Mother vaginal microbes contribute to microbiome of vaginally delivered neonates. Child can be associated with autoimmune diseases, such as type 1 diabetes (T1D). We collected DNA samples from 25 mothers a child diagnosed T1D and 24 control who had healthy analyzed bacteriome mycobiome the samples. The total was extracted, ribosomal regions (16S for bacteria, ITS2 fungi) were amplified, followed by next-generation sequencing machine learning. found that alpha-diversity increased (P < 0.002),...
A number of proteins that act as necessary catalysts for correct protein folding and oligomerization in the endoplasmic reticulum (ER) are known to be retained organelle via KDEL-receptor mediated retrieval mechanism. However, a complementary system may help retain these lumen has been suggested exist likely involves physical protein-protein interactions at level itself. In this report, we provide both morphological biochemical evidence support proposal. We show collagen-secreting human skin...
Abstract Background Bacterial extracellular vesicles (EVs) are more likely to cross biological barriers than whole-cell bacteria. We previously observed EV-sized particles by electron microscopy in the first-pass meconium of newborn infants. hypothesized that EVs may be bacterial origin and represent a novel entity human microbiome during fetal perinatal periods. Methods extracted from samples 17 infants performed 16S rRNA gene sequencing vesicles. compared EV content based on delivery mode,...
Food adulteration has been an issue in the food industry for many decades. The market natural and healthy products expanding therefore value of raw ingredients harvested from sources increased, and, some cases, it led to such high-valued products. Berry fruits different species genus Vaccinium have gaining worldwide popularity because their nutraceutical benefits. However, there are increasing reports or misidentification high-value wild berries with cultivated counterparts. During recent...
Bilberry (Vaccinium myrtillus L.) fruits are an excellent natural resource for human diet because of their special flavor, taste and nutritional value as well medical properties. Bilberries recognized high anthocyanin content many the genes involved in biosynthesis have been characterized. So far, neither genomic nor RNA-seq data available species. In present study, we de novo sequenced two bilberry fruit developmental stages, unripe green (G) ripening (R). A total 57,919 unigenes were...
The development of cost-effective treatment methods for nutrient-rich effluents is great importance. In this study, microalgal-based technology was investigated as a final polishing stage fish processing wastewater (FPWW) after fat and oil removal, with the purpose facilitating reuse nutrient recovery. First, effect FPWW filtration on growth two microalgal species (Chlorella sorokiniana Selenastrum sp.) under batch mode. For both microalgae, biomass concentration removal levels were higher...
SUMMARY Na + -independent Cl − /HCO 3 exchangers (AE1, AE2, AE3) are generally known as ubiquitous, multispanning plasma membrane proteins that regulate intracellular pH and transepithelial acid–base balance in animal tissues. However, previous immunological evidence has suggested anion exchanger (AE) may also be present membranes, including membranes of the Golgi complex mitochondria. Here we provide several lines to show an AE protein is indeed a resident this corresponds full-length AE2a...
Unlike in flowering plants, the detailed roles of enzymes polyamine (PA) pathway conifers are poorly known. We explored sequence conservation PA biosynthetic genes and diamine oxidase (DAO) plants to reveal potential functional diversification between plant lineages. The expression showing different selective constraints was studied Scots pine zygotic embryogenesis early seedling development. found that arginine decarboxylase (ADC) is strongly preferred putrescine production as well...
The development of DNA-based methods in recent decades has opened the door to numerous new lines research biological sciences. While speed and accuracy DNA methodologies are clearly beneficial, sensitivity these adverse effect increased susceptibility false positives resulting from contamination field or lab. Here, we present findings a metabarcoding study on diet food availability for five insectivorous birds, which multiple lepidopteran species not known occur locally were discovered....
During environmental changes, epigenetic processes can enable adaptive responses faster than natural selection. In plants, very little is known about the role of DNA methylation during long-term adaptation. Scots pine a widely distributed coniferous species which must adapt to different conditions throughout its long lifespan. Thus, modifications may contribute towards this direction. We provide bisulfite next-generation sequencing data from putative promoters and exons eight...
Lysyl hydroxylase (LH) is a peripheral membrane protein in the lumen of endoplasmic reticulum (ER) that catalyses hydroxylation lysine residues collagenous sequences. Previously, we have mapped its primary ER localization motif within 40-amino acid segment at C-terminus. Here, characterized this mechanism more detail, and our results indicate confers residency KDEL-receptor-independent manner, without any apparent recycling enzyme between Golgi apparatus ER. In addition, show rather long...
Hydroxylation of lysyl residues is crucial for the unique glycosylation pattern found in collagens and mechanical strength fully assembled extracellular collagen fibers. catalyzed lumen endoplasmic reticulum (ER) by a specific enzyme, hydroxylase (LH). The absence known ER-specific retrieval motifs its primary structure association with ER membranes <i>in vivo</i> have suggested that enzyme localized via novel retention/retrieval mechanism. We identified here 40-amino acid C-terminal peptide...