A5055612323- Probiotics and Fermented Foods
- Gut microbiota and health
- Biochemical and Structural Characterization
- Microbial Metabolites in Food Biotechnology
- Infant Nutrition and Health
- Extracellular vesicles in disease
- Bacterial Infections and Vaccines
- Clostridium difficile and Clostridium perfringens research
- Genomics and Phylogenetic Studies
- Dental materials and restorations
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Digestive system and related health
- Antimicrobial Peptides and Activities
- Peptidase Inhibition and Analysis
- Protease and Inhibitor Mechanisms
- Dental Implant Techniques and Outcomes
- Bone Tissue Engineering Materials
- Facial Trauma and Fracture Management
- Urinary Tract Infections Management
- Microbial Inactivation Methods
- Biochemical Analysis and Sensing Techniques
- Milk Quality and Mastitis in Dairy Cows
- Adolescent and Pediatric Healthcare
University of Oulu
2017-2024
Northern Ostrobothnia Hospital District
2022
University of Helsinki
2010-2021
To unravel the biological function of widely used probiotic bacterium Lactobacillus rhamnosus GG, we compared its 3.0-Mbp genome sequence with similarly sized L. LC705, an adjunct starter culture exhibiting reduced binding to mucus. Both genomes demonstrated high identity and synteny. However, for both strains, genomic islands, 5 in GG 4 punctuated colinearity. A significant number strain-specific genes were predicted these islands (80 72 LC705). The GG-specific included coding bacteriophage...
Akkermansia muciniphila is a Gram-negative mucin-degrading bacterium that resides in the gastrointestinal tracts of humans and animals. A. has been linked with intestinal health improved metabolic status obese type 2 diabetic subjects. Specifically, shown to reduce high-fat-diet-induced endotoxemia, which develops as result an impaired gut barrier. Despite accumulating evidence health-promoting effects muciniphila, mechanisms interaction host have received little attention. In this study, we...
Gut barrier function is key in maintaining a balanced response between the host and its microbiome. The microbiota can modulate changes gut as well metabolic inflammatory responses. This highly complex system involves numerous microbiota-derived factors. symbiont Akkermansia muciniphila positively correlated with lean phenotype, reduced body weight gain, amelioration of responses restoration by modulation mucus layer thickness. However, molecular mechanisms behind immunological regulatory...
Development of the human gut microbiota commences at birth, with bifidobacteria being among first colonizers sterile newborn gastrointestinal tract. To date, genetic basis Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis breve UCC2003 2.42-Mb genome in a murine model revealed differential expression type IVb tight adherence (Tad) pilus-encoding gene cluster designated “ tad 2003 .” Mutational demonstrated that is essential for efficient vivo...
ABSTRACT Lactobacillus rhamnosus GG, a probiotic with good survival capacity in the human gut, has well-documented adhesion properties and health effects. Recently, spaCBA -encoded pili that bind to intestinal mucus were identified on its cell surface. Here, we report phenotypic analysis of pilus knockout mutant comparison wild type other adhesin mutants. The SpaCBA L. GG showed be key for efficient adherence Caco-2 epithelial (IEC) line biofilm formation. Moreover, induces an elevated level...
Lactobacillus rhamnosus is a lactic acid bacterium that found in large variety of ecological habitats, including artisanal and industrial dairy products, the oral cavity, intestinal tract or vagina. To gain insights into genetic complexity versatility species L. rhamnosus, we examined genomes phenotypes 100 strains isolated from diverse sources. The were mapped onto GG reference genome. These phenotypically characterized for wide range metabolic, antagonistic, signalling functional...
Lactobacillus rhamnosus GG is a well-established Gram-positive probiotic strain, whose health-benefiting properties are dependent in part on prolonged residence the gastrointestinal tract and likely dictated by adherence to intestinal mucosa. Previously, we identified two pilus gene clusters (spaCBA spaFED) genome of this bacterium, each which contained predicted genes for three pilin subunits single sortase. We also confirmed presence SpaCBA pili cell surface attributed an mucus-binding...
ABSTRACT Lactobacillus rhamnosus GG is a human intestinal isolate that has been studied intensively because of its probiotic properties. We have previously shown L. produces proteinaceous pili earlier had observed only in Gram-positive pathogens (M. Kankainen et al., Proc. Natl. Acad. Sci. U. S. A. 106: 17193–17198, 2009). These were found to be encoded by the spaCBA gene cluster, and pilus-associated SpaC pilin was confer on cells mucus-binding ability. In addition another putative pilus...
Four Lactobacillus strains were isolated from marketed probiotic products, including L. rhamnosus Vifit (Friesland Campina) and Idoform (Ferrosan) casei Actimel (Danone) Yakult (Yakult Honsa Co.). Their genomes phenotypes characterized compared in detail with strain BL23 GG. Phenotypic analysis of the new isolates indicated differences carbohydrate utilization between strains, which could be linked to their genotypes. The two had that virtually identical GG, testifying genomic stability...
Akkermansia muciniphila is a common member of the human gut microbiota and belongs to Planctomycetes-Verrucomicrobia-Chlamydiae superphylum. Decreased levels A. have been associated with many diseases, thus it considered be beneficial resident intestinal mucus layer. Surface-exposed molecules produced by this organism likely play important roles in colonization communication other microbes host, but protein composition outer membrane (OM) has not characterized far. Herein we set out identify...
Reports regarding the presence of bacteria in fetal environment remain limited and controversial. Recently, extracellular vesicles secreted by human gut microbiota have emerged as a novel mechanism for host-microbiota interaction. We aimed to investigate bacterial during healthy pregnancies determine whether derived from can cross biological barriers reach fetus.Bacterial were detectable amniotic fluid pregnant women, exhibiting similarities found maternal microbiota. In mice, intra-amniotic...
Gut microbiome–derived nanoparticles, known as bacterial extracellular vesicles (bEVs), have garnered interest promising tools for studying the link between gut microbiome and human health. The diverse composition of bEVs, including their proteins, mRNAs, metabolites, lipids, makes them useful investigating diseases such cancer. However, conventional approaches alone may not be accurate in deciphering host–gut communication. In clinical research, there is a gap knowledge on role bEVs solid...
In spite of the wealth clinical evidence supporting health benefits Lactobacillus rhamnosus GG in humans, there is still a lack understanding molecular mechanisms behind its probiosis. Current knowledge suggests that health-promoting effects this probiotic strain might be partly dependent on persistence intestine and adhesion to mucosal surfaces. Moreover, L. contains mucus-binding pili also explain occupation ecological niche as comparatively less stringent allochthonous intestine-dwelling...
Vancomycin-resistant enterococci (VRE) have become a major nosocomial threat. Enterococcus faecium is of special concern, as it can easily acquire new antibiotic resistances and an excellent colonizer the human intestinal tract. Several clinical studies explored potential use beneficial bacteria to weed out opportunistic pathogens. Specifically, widely studied Lactobacillus rhamnosus strain GG has been applied successfully in context VRE infections. Here, we provide insight into molecular...
Lactobacillus rhamnosus GG (LGG) produces two major secreted proteins, designated here Msp1 (LGG_00324 or p75) and Msp2 (LGG_00031 p40), which have been reported to promote the survival growth of intestinal epithelial cells. Intriguingly, although each these proteins shares homology with cell wall hydrolases, a physiological function that correlates such an enzymatic activity remained be substantiated in LGG. To investigate bacterial function, we constructed knock-out mutants corresponding...
Abstract Development of the human gut microbiota commences at birth, with certain bifidobacterial species representing dominant and early colonisers newborn gastrointestinal tract. The molecular basis Bifidobacterium colonisation, persistence presumed communication host has remained obscure. We previously identified tight adherence (Tad) pili from breve UCC2003 as an essential colonisation factor. Here, we demonstrate that Tad also promote in vivo colonic epithelial proliferation. A...
Abstract Background The composition of the gut fungal microbiome, mycobiome, is likely associated with human health. Yet, development mycobiome poorly understood in infants and children. Here we investigate how perinatal events influence mycobiome. Methods In this prospective cohort study 140 infants, used ITS gene sequencing fecal samples from birth to age 18 months. We compared according delivery mode exposure intrapartum antibiotics during vaginal delivery. Results At birth, were...
Primarily arising from their well understood beneficial health effects, many lactobacilli strains are considered good candidates for use as probiotics in humans and animals. Lactobacillar probiosis can itself be best typified by the Lactobacillus rhamnosus GG strain, which, with its well-documented clinical benefits, has emerged one of most widely used food health-supplement industries. Even so, facets molecular mechanisms limitations a commensal bacterium still remain to thoroughly explored...
Mapping of the microbial molecules underlying microbiota-host interactions is key to understand how microbiota preserve mucosal homeostasis. A pivotal family such bacterial are pili. Pili proteinaceous cell wall appendages with a well-documented role in adhesion, whilst their immune interaction host less established. Gram-positive pili often posttranslationally modified by sortase-specific cleavage reactions and formation intramolecular peptide bonds. Here we report glycosylation as new...
Abstract We critically evaluated the fetal microbiome concept in 44 neonates with placenta, amniotic fluid, and first-pass meconium samples. Placental histology showed no signs of inflammation. Meconium samples were more often bacterial culture positive after vaginal delivery. In next-generation sequencing 16S gene, before removal extracellular PCR contaminant DNA, median number reads was low placenta (48) fluid (46) high (14,556 C-section, 24,860 vaginal). electron microscopy, vesicles....
For a good probiotic candidate, the abilities to adhere intestinal epithelium and fortify barrier function are considered be crucial for colonization functionality of strain. The strain Lactobacillus acidophilus LAB20 was isolated from jejunum healthy dog, where it found most pre-dominant lactobacilli. In this study, adhesion ability epithelial cell (IECs) lines, IECs canine biopsies, canine, porcine human mucus investigated. Further, we studied monolayer reduce LPS-induced interleukin...
The present study reports the identification and comparison of all expressed cell-surface exposed proteins from well-known probiotic L. rhamnosus GG a related dairy strain, Lc705. To obtain this information, bound were released intact cells by trypsin shaving under hypertonic conditions with without DTT. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analyses purified peptides identified total 102 198 individual Lc705, respectively. Comparison both data sets suggested that...
This study investigated two of the most commonly used CAD-CAM materials for patient-specific reconstruction in craniomaxillofacial surgery. The aim this was to access biofilm formation Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis, and Escherichia coli on titanium PEEK medical implant materials. Two specimens (titanium grade 2 tooled with a Planmeca milling device 5 computer-aided design direct metal laser sintering (CAD-DMLS)) one specimen were studied. Bacterial...