A5009906360- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Protein Hydrolysis and Bioactive Peptides
- Gut microbiota and health
- Polyamine Metabolism and Applications
- RNA and protein synthesis mechanisms
- Biopolymer Synthesis and Applications
- Bacterial biofilms and quorum sensing
- Biochemical Analysis and Sensing Techniques
- Microbial Metabolism and Applications
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Infant Nutrition and Health
- Listeria monocytogenes in Food Safety
- Fermentation and Sensory Analysis
- Microbial Inactivation Methods
- Monoclonal and Polyclonal Antibodies Research
- Oral microbiology and periodontitis research
- Glycosylation and Glycoproteins Research
- Protease and Inhibitor Mechanisms
- Food composition and properties
University of Helsinki
2015-2024
Antibiotic Research UK
2007
Biocenter Finland
1996-2004
Helsinki Institute of Physics
1991-1995
Valio (Finland)
1992
Institute for Molecular Medicine Finland
1990
To study the bacterial diversity in expressed human milk with a focus on detecting bacteria an antimicrobial activity against Staphylococcus aureus, known as causative agent of maternal breast infections and neonatal infections.Random isolates (n = 509) were collected from samples 40) healthy lactating women, genotypically identified, tested for Staph. aureus. Commensal staphylococci (64%) oral streptococci (30%), epidermidis, Strep. salivarius, mitis most frequent isolates, predominant...
The implementation of novel chassis organisms to be used as microbial cell factories in industrial applications is an intensive research field. Lactococcus lactis, which one the most extensively studied model organisms, exhibits superior ability engineered host for fermentation desirable products. However, few studies have reported about genome reduction L. lactis a clean background functional genomic and product fermentation.Four large nonessential DNA regions accounting 2.83% NZ9000 (L. 9...
Parkinson’s disease (PD) is the most prevalent movement disorder known and predominantly affects elderly. It a progressive neurodegenerative wherein α-synuclein, neuronal protein, aggregates to form toxic structures in nerve cells. The cause of remains unknown. Intestinal dysfunction changes gut microbiota, common symptoms PD, are evidently linked pathogenesis PD. Although multitude studies have investigated microbial etiologies role progression unclear. Here, we show that Gram-negative...
Introduction The aggregation of the neuronal protein alpha-synuclein (alpha-syn) is a key feature in pathology Parkinson’s disease (PD). Alpha-syn has been suggested to be induced gut cells by pathogenic microbes such as Desulfovibrio bacteria, which shown associated with PD. This study aimed investigate whether bacteria induce alpha-syn aggregation. Methods Fecal samples ten PD patients and their healthy spouses were collected for molecular detection species, followed bacterial isolation....
Characterization by partial 16S rRNA gene sequencing, ribotyping, and green fluorescent protein-based nisin bioassay revealed that 6 of 20 human milk samples contained nisin-producing Lactococcus lactis bacteria. This suggests the history humans consuming is older than tradition fermented products.
Summary A gene locus of Bacillus subtilis identified by mutations (prs) conferring a defect in protein secretion was cloned from lambdaGEM‐11 expression library. The sites three closely linked prs ( prs‐3, prs‐29 and prs‐40 ) were found to reside 5.3kb DNA fragment, which also complemented the prs‐3 mutants. Partial sequencing fragment showed that these affect one distinct encoding putative 292 amino acids (33 kDa). Sequence analysis Indicated PrsA be lipoprotein located outside cytoplasmic...
Endophytic fungi and bacteria were isolated from finger millet their effects on growth parameters zinc NPK contents in grains studied. Out of 70 fungal 112 bacterial endophytes, the two best isolates selected basis solubilization plant-growth-promoting attributes. The identified Aspergillus terreus Lecanicillium sp., Pseudomonas bijieensis Priestia megaterium. endophytic zinc, mobilization, efficacy determined a pot experiment with carbonate as source. Endophytic-primed plants showed...
ABSTRACT Bacteria from crops of 1- and 5-week-old broiler chickens fed with two brands (diets A B) wheat-based diets were isolated on Lactobacillus -selective medium identified ( n = 300) based partial 16S rRNA gene sequence. The most abundant species L. reuteri (33%), crispatus (18.7%), salivarius (13.3%). Regardless farm feed, was the P < 0.005) in younger chickens. However, amount significantly reduced regardless feed 0.016). diversity isolates studied by fatty acid analysis, 94 could...
Abstract Today, a few hundred mycotoxins have been identified and the number is rising. Mycotoxin detoxification of food feed has technically uphill task for industry. In twenty-first century, public demand healthy with minimum use chemicals preservatives. Among all fungal inhibition mycotoxin methods so far developed food, biopreservation biodetoxification found safe reliable. Nowadays, lactic acid bacteria (LAB) are great interest as biological additives in owing to their Generally...
The lantibiotic nisin is produced by several strains of Lactococcus lactis subsp. lactis. chromosomally located gene cluster nisABTCIPRKFEG required for biosynthesis, development immunity, and regulation expression. Inframe deletions in the nisB nisT genes, disruption nisC plasmid integration, eliminated production resulted a strongly reduced level immunity strains. transcription two operons was inactivated these mutant strains, but could be restored addition small amounts to growing...
Nisin produced by Lactococcus lactis subsp. is a 34-residue antibacterial polypeptide and belongs to group of post-translationally modified peptides, lantibiotics, with dehydrated residues cyclic amino acids, lanthionines. These modifications are supposed be made enzymes encoded lanB lanC genes, found only in biosynthetic operons encoding lantibiotics. To analyse the extent modification, His-tagged nisin precursors were expressed nisB nisC mutant strains. The purified from cytoplasm cells,...
Nisin is a small post-translationally modified lanthionine-containing peptide (lantibiotic) produced by certain Lactococcus lactis strains which has high antimicrobial activity against several pathogenic Gram-positive bacteria. Northern blots and RT/PCR analyses of the nisin-producing strain N8 revealed that nisZBTCIPRKFEG gene cluster, responsible for nisin biosynthesis, immunity regulation, consists two operons, nisZBTCIPRK nisFEG . The promoter operon was mapped. −35 to −1 region upstream...
For a good probiotic candidate, the abilities to adhere intestinal epithelium and fortify barrier function are considered be crucial for colonization functionality of strain. The strain Lactobacillus acidophilus LAB20 was isolated from jejunum healthy dog, where it found most pre-dominant lactobacilli. In this study, adhesion ability epithelial cell (IECs) lines, IECs canine biopsies, canine, porcine human mucus investigated. Further, we studied monolayer reduce LPS-induced interleukin...
The synthesis of heterologous proteins in Lactococcus lactis is strongly influenced by the promoter selected for expression. nisin A commonly used induced expression L. lactis, whereas few constitutive promoters (P45 and weaker P32) have been protein studies. In this study, eight different putative strong were identified through transcriptional analysis N8 investigated their capability to drive nisZ gene with P45 P32 as control. Four (P8, P5, P3 P2) having a activity that was higher than...
ABSTRACT To overcome the adverse impacts of environmental stresses during growth, different adaptive regulation mechanisms can be activated in Lactococcus lactis . In this study, transcription levels eight transcriptional regulators L. subsp. F44 under acid stress were analyzed using quantitative reverse transcription-PCR. Eight gene-overexpressing strains then constructed to examine their influences on acid-resistant capability. Overexpressing ythA , a PspC family regulator, increased...