A5008533865- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Listeria monocytogenes in Food Safety
- Microbial Community Ecology and Physiology
- Microbial Inactivation Methods
- Bacteriophages and microbial interactions
- Dysphagia Assessment and Management
- RNA and protein synthesis mechanisms
- Plant Pathogens and Fungal Diseases
- Microbial Metabolic Engineering and Bioproduction
- Parkinson's Disease Mechanisms and Treatments
- Dermatology and Skin Diseases
- Gene expression and cancer classification
- Plant-Microbe Interactions and Immunity
- Viral Infections and Immunology Research
- Enzyme-mediated dye degradation
- Plant Pathogenic Bacteria Studies
- Fungal Biology and Applications
- Cardiomyopathy and Myosin Studies
- Genetic diversity and population structure
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
Helsinki Institute of Physics
2010-2025
University of Helsinki
2016-2025
Czech Academy of Sciences, Institute of Biotechnology
2011-2024
Institute of Life Sciences
2024
HiLIFE – Elämäntieteiden Instituutti
2024
Helsinki Art Museum
2019-2022
Emory University
2021
Norwegian University of Life Sciences
2021
Université de Montpellier
2020
Inserm
2020
In the course of Parkinson's disease (PD), enteric nervous system (ENS) and parasympathetic nerves are amongst structures earliest most frequently affected by alpha-synuclein pathology. Accordingly, gastrointestinal dysfunction, in particular constipation, is an important non-motor symptom PD often precedes onset motor symptoms years. Recent research has shown that intestinal microbiota interact with autonomic central via diverse pathways including ENS vagal nerve. The gut microbiome not...
Rapidly declining biodiversity may be a contributing factor to another global megatrend—the rapidly increasing prevalence of allergies and other chronic inflammatory diseases among urban populations worldwide. According the “biodiversity hypothesis,” reduced contact people with natural environmental features adversely affect human commensal microbiota its immunomodulatory capacity. Analyzing atopic sensitization (i.e., allergic disposition) in random sample adolescents living heterogeneous...
To unravel the biological function of widely used probiotic bacterium Lactobacillus rhamnosus GG, we compared its 3.0-Mbp genome sequence with similarly sized L. LC705, an adjunct starter culture exhibiting reduced binding to mucus. Both genomes demonstrated high identity and synteny. However, for both strains, genomic islands, 5 in GG 4 punctuated colinearity. A significant number strain-specific genes were predicted these islands (80 72 LC705). The GG-specific included coding bacteriophage...
Abstract Background Composting is an aerobic microbiological process that facilitated by bacteria and fungi. also a method to produce fertilizer or soil conditioner. Tightened EU legislation now requires treatment of the continuously growing quantities organic municipal waste before final disposal. However, some full-scale composting plants experience difficulties with efficiency biowaste degradation emission noxious odours. In this study we examine bacterial species richness community...
Despite the high prevalence of nonalcoholic fatty liver disease (NAFLD), little is known its pathogenesis based on study human samples. By use Affymetrix GeneChips (17,601 genes), we investigated gene expression in subjects with extreme steatosis due to NAFLD without histological signs inflammation (liver fat 66.0 +/- 6.8%) and low content (6.4 2.7%). The data were analyzed by using sequence-based reannotation probes a robust model-based normalization method. We identified genes involved...
Abstract Background Previous studies have reported that gut microbiota, permeability, short-chain fatty acids (SCFAs), and inflammation are altered in Parkinson’s disease (PD), but how these factors linked they contribute to processes symptoms remains uncertain. This study sought compare identify associations among PD patients controls elucidate their interrelations links clinical manifestations of PD. Methods Stool plasma samples data were collected from 55 56 controls. Levels stool SCFAs...
Several publications have described differences in cross-sectional comparisons of gut microbiota between patients with Parkinson's disease and control subjects, considerable variability the reported differentially abundant taxa. The temporal stability such alterations their relationship to progression not been previously studied a high-throughput sequencing based approach.We collected clinical data stool samples from 64 subjects twice, on average 2·25 years apart. Disease was evaluated...
Silver birch (Betula pendula) is a pioneer boreal tree that can be induced to flower within 1 year. Its rapid life cycle, small (440-Mb) genome, and advanced germplasm resources make an attractive model for forest biotechnology. We assembled chromosomally anchored the nuclear genome of inbred B. pendula individual. Gene duplicates from paleohexaploid event were enriched transcriptional regulation, whereas tandem overrepresented by environmental responses. Population resequencing 80...
Western lifestyle is associated with high prevalence of allergy, asthma and other chronic inflammatory disorders. To explain this association, we tested the 'biodiversity hypothesis', which posits that reduced contact children environmental biodiversity, including microbiota in natural habitats, has adverse consequences on assembly human commensal its contribution to immune tolerance.We analysed four study cohorts from Finland Estonia (n = 1044) comprising adolescents aged 0.5-20 years. The...
Previous studies have reported that chromosome synteny in Lepidoptera has been well conserved, yet the number of haploid chromosomes varies widely from 5 to 223. Here we report genome (393 Mb) Glanville fritillary butterfly (Melitaea cinxia; Nymphalidae), a recognized model species metapopulation biology and eco-evolutionary research, which putative ancestral karyotype n=31. Using phylogenetic analyses Nymphalidae other Lepidoptera, combined with orthologue-level comparisons chromosomes,...
Photoassimilates such as sugars are transported through phloem sieve element cells in plants. Adapted for effective transport, elements develop enucleated living cells. We used electron microscope imaging and three-dimensional reconstruction to follow morphogenesis Arabidopsis. show that differentiation involves enucleation, which the nuclear contents released degraded cytoplasm at same time other organelles rearranged cytosol is degraded. These cellular reorganizations orchestrated by...
The gut microbiome and its metabolites can impact brain health are altered in Parkinson's disease (PD) patients. It has been recently demonstrated that PD patients have reduced fecal levels of the potent epigenetic modulator butyrate bacterial producers.Here, we investigate whether changes associated related to symptoms markers leucocytes neurons.Stool, whole blood samples, clinical data were collected from 55 controls. We performed DNA methylation analysis on samples analyzed results...
Although echovirus 22 is presently classified as a member of the enterovirus group in family picornaviruses, it has been reported to have exceptional biological properties when compared with other representatives group. We determined complete nucleotide sequence (Harris strain) genome, which appears be significantly different from all studied picornaviruses. However, organization genome [7339 nucleotides, excluding poly(A) tract] similar that previously sequenced This includes 5'...
ABSTRACT The nonstructural (ns) proteins nsP1 to -4, the components of Semliki Forest virus (SFV) RNA polymerase, were localized in infected cells by confocal microscopy using double labeling with specific antisera against individual ns proteins. All associated large cytoplasmic vacuoles (CPV), inner surfaces which covered small invaginations, or spherules, typical alphavirus infection. immuno-electron (EM) limiting membranes CPV and together newly labeled viral RNA. Along earlier...
Rab8 is a small Ras-like GTPase that regulates polarized membrane transport to the basolateral in epithelial cells and dendrites neurons. It has recently been demonstrated fibroblasts sort newly synthesized proteins into two different pathways for delivery cell surface are equivalent apical post-Golgi routes (Yoshimori, T., P. Keller, M.G. Roth, K. Simons. 1996. J. Cell Biol. 133:247-256). To determine role of fibroblasts, we used both transient expression systems stable lines expressing...
Abstract Background Plants growing in their natural habitat represent a valuable resource for elucidating mechanisms of acclimation to environmental constraints. Populus euphratica is salt-tolerant tree species saline semi-arid areas. To identify genes involved abiotic stress responses under conditions we constructed several normalized and subtracted cDNA libraries from control, stress-exposed desert-grown P. trees. In addition, identified metabolites Results About 14,000 expressed sequence...
SUMMARY We have used enzymic amplification of specific nucleic acid sequences followed by hybridization, for the rapid detection and typing human picornaviruses after cell culture isolation. The test is based on synthesis cDNA, polymerase chain reaction use oligonucleotide probes. primers were selected from 5′ non-coding region genome representing highly conserved regions. Sequences to enteroviruses rhinoviruses as assay was able identify all picornavirus reference strains analysed it also...
Both genomic and subgenomic RNAs of theAlphavirus have m7G(5′)ppp(5′)N (cap0 structure) at their 5′ end. Previously it has been shown thatAlphavirus-specific nonstructural protein Nsp1 guanine-7N-methyltransferase guanylyltransferase activities needed in the synthesis cap structure. During normal γ-phosphate nascent viral RNA chain is removed by a specific 5′-triphosphatase before condensation with GMP, delivered guanylyltransferase. Using novel triphosphatase assay, we show here that Nsp2...
In recent years increasing attention has been given to the potential health effects of fungal exposure in indoor environments. We used large-scale sequencing internal transcribed spacer region (ITS) nuclear ribosomal DNA describe mycoflora two office buildings over four seasons. was complemented by cultivation, ergosterol determination, and quantitative PCR analyses. Sequences 1,339 clones were clustered into 394 nonredundant operational taxonomical units containing sequences from 18...
Cdc42 and Rac1 are members of the rho family small guanosinetriphosphatases required for a diverse set cytoskeleton-membrane interactions in different cell types. Here we show that these two proteins contribute differently to organization epithelial cells Drosophila wing imaginal disc. Drac1 is assemble actin at adherens junctions. Failure junction assembly dominant-negative mutants associated with increased death. Dcdc42, on other hand, processes involve polarized shape changes during both...