A5081232087- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Bioenergy crop production and management
- Plant nutrient uptake and metabolism
- Plant Water Relations and Carbon Dynamics
- Polysaccharides and Plant Cell Walls
- Plant Parasitism and Resistance
- Biofuel production and bioconversion
- Genetic and Environmental Crop Studies
- Light effects on plants
- Plant and animal studies
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
- Plant responses to water stress
- Fungal and yeast genetics research
- Plant tissue culture and regeneration
- Plant Ecology and Soil Science
- Soybean genetics and cultivation
- Cellular transport and secretion
- Seed Germination and Physiology
- Biochemical and Structural Characterization
University of Göttingen
2008-2020
GTx (United States)
2013
University of Helsinki
2005-2006
Ecologie et Ecophysiologie Forestières
2006
Université de Lorraine
2006
Beijing Forestry University
2005
Institut Für Angewandte Kulturforschung
2005
University of Würzburg
1994-1997
Max Planck Institute for the Study of Societies
1997
Abstract To investigate whether Cd induces common plant defense pathways or unspecific necrosis, the temporal sequence of physiological reactions, including hydrogen peroxide (H2O2) production, changes in ascorbate-glutathione-related antioxidant systems, secondary metabolism (peroxidases, phenolics, and lignification), developmental changes, was characterized roots hydroponically grown Scots pine (Pinus sylvestris) seedlings. (50 μm, 6 h) initially increased superoxide dismutase, inhibited...
Abstract The responses of Populus euphratica Oliv. plants to soil water deficit were assessed by analyzing gene expression, protein profiles, and several plant performance criteria understand the acclimation deficit. Young, vegetatively propagated originating from an arid, saline field site submitted a gradually increasing for 4 weeks in greenhouse allowed recover 10 d after full reirrigation. Time-dependent changes intensity perturbations induced shoot root growth, xylem anatomy, gas...
Endocytosis is a crucial mechanism by which eukaryotic cells internalize extracellular and plasma membrane material, it required for multitude of cellular developmental processes in unicellular multicellular organisms. In animals yeast, the best characterized pathway endocytosis depends on function vesicle coat protein clathrin. Clathrin-mediated has recently been demonstrated also plant cells, but its physiological roles remain unclear. Here, we assessed clathrin-mediated plants genetic...
Gradients of the plant hormone auxin, which depend on its active intercellular transport, are crucial for maintenance root meristematic activity. This directional transport is largely orchestrated by a complex interaction specific influx and efflux carriers that mediate auxin flow into out cells, respectively. Besides these proteins, plant-specific polyphenolic compounds known as flavonols have been shown to act endogenous regulators transport. However, only limited information available how...
Abstract Populus euphratica Olivier is known to exist in saline and arid environments. In this study we investigated the physiological mechanisms enabling species cope with stress caused by salinity. Acclimation increasing Na+ concentrations required adjustments of osmotic pressure leaves, which were achieved accumulation compensatory decreases calcium soluble carbohydrates. The counterbalance Na+/Ca2+ was also observed mature leaves from field-grown P. trees exposed an environmental...
Abstract Background Plants growing in their natural habitat represent a valuable resource for elucidating mechanisms of acclimation to environmental constraints. Populus euphratica is salt-tolerant tree species saline semi-arid areas. To identify genes involved abiotic stress responses under conditions we constructed several normalized and subtracted cDNA libraries from control, stress-exposed desert-grown P. trees. In addition, identified metabolites Results About 14,000 expressed sequence...
ABSTRACT To investigate the physiological basis of salt adaptation in poplar, we compared effect stress on wood anatomy and auxin physiology salt‐resistant Populus euphratica salt‐sensitive × canescens . Both poplar species showed decreases vessel lumina associated with increases wall strength response to salt, however, P. at three‐fold higher concentrations than P The predicted hydraulic conductivity formed under decreased , while no significant effects transpiration were observed....
The functions of the minor phospholipid phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] during vegetative plant growth remain obscure. Here, we targeted two related phosphatidylinositol 4-phosphate 5-kinases (PI4P 5-kinases) PIP5K1 and PIP5K2, which are expressed ubiquitously in Arabidopsis thaliana. A pip5k1 pip5k2 double mutant with reduced PtdIns(4,5)P2 levels showed dwarf stature phenotypes suggesting defects auxin distribution. roots had normal but transport altered...
Gravitropic bending of plant organs is mediated by an asymmetric signaling the hormone auxin between upper and lower side respective organ. Here, we show that also another hormone, gibberellic acid (GA), shows action during gravitropic responses. Immunodetection using antibody against GA monitoring output downstream degradation DELLA proteins revealed distribution response with maximum at gravistimulated roots. Genetic or pharmacological manipulation levels affects gravity-mediated...
Abstract The soilborne fungal plant pathogen Verticillium longisporum invades the roots of its Brassicaceae hosts and proliferates in vascular system. Typical aboveground symptoms infection on Brassica napus Arabidopsis thaliana are stunted growth, vein clearing, leaf chloroses. Here, we provide evidence that clearing is caused by pathogen-induced transdifferentiation chloroplast-containing bundle sheath cells to functional xylem elements. In addition, our findings suggest reinitiation...
Abstract: Trees, due to their long life‐span, have characteristics that distinguish them from annual, herbaceous plants. It is likely many of these properties are based on a tree‐specific genetic foundation. The U.S. Department Energy initiated genome‐sequencing project for Populus, model perennial plant. Through international collaboration and input the sequencing effort, annotated whole genome sequence Populus trichocarpa will be released public in early 2004. This genomic resource will,...
Summary Plant architecture is modified by a regulatory system that controls axillary bud outgrowth. Key components in this are strigolactones (SLs) and BRANCHED 1 , which inhibit Their role has been described herbaceous model systems, including Arabidopsis rice pea. However, woody perennial species, the tree poplar, not unequivocally proven. In study, we tested for SLs Populus × canescens treatment with synthetic SL GR 24. We generated MORE AXILLARY BRANCHING 4 ( MAX ) knockdown lines to...
Verticillium longisporum is a vascular pathogen that infects the Brassicaceae host plants Arabidopsis thaliana and Brassica napus. The soilborne fungus enters plant via roots colonizes xylem of roots, stems, leaves. During late stages infections, spp. spread into senescing tissue switch from biotrophic to necrotrophic life style. Typical symptoms V. longisporum-induced disease are stunted growth leaf chlorosis. Expression analyses senescence marker genes SENESCENCE-ASSOCIATED GENE12, GENE13,...
The TCP-type transcription factors BRANCHED1 and BRANCHED2 shape plant architecture by suppressing bud outgrowth, with only playing a minor role in Arabidopsis. Here, we investigated the function of orthologs these genes model tree Populus. We used CRISPR/Cas9 to generate loss-of-function mutants previously identified Populus BRANCHED1-1 BRANCHED2-1 candidate genes. exhibited strongly enhanced outgrowth. had an extreme outgrowth phenotype possessed two ectopic leaves at each node. While is...
Abstract Subdiffraction super‐resolution fluorescence microscopy, or nanoscopy, has seen remarkable developments in the last two decades. Yet, for visualization of plant cells, nanoscopy is still rarely used. In this study, we established RESOLFT on living green tissue. Live‐cell requires and utilizes comparatively low light doses intensities to overcome diffraction barrier. We generated a transgenic Arabidopsis thaliana line expressing reversibly switchable fluorescent protein rsEGFP2 fused...
The first leaky auxotrophic mutant for aromatic amino acids of the near-diploid fungal plant pathogen Verticillium longisporum (VL) has been generated. VL enters its host Brassica napus through roots and colonizes xylem vessels. contains little nutrients including low concentrations acids. We isolated gene Vlaro2 encoding chorismate synthase by complementation corresponding yeast strain. Chorismate produces branch point intermediate acid biosynthesis. A novel RNA-mediated silencing method...
Black cottonwood (BC, Populus trichocarpa) and hybrid aspen (HA, P. tremula × tremuloides) differ in their ecology of being adapted to wet drier conditions as riparian early successional forest species, respectively. We tested the hypothesis that these ecological differences were reflected higher nitrogen (N) use efficiency HA than BC would allocate more resources belowground presence high low N availability. expected responses wood properties elevated be pronounced species with formation...