A5021003355- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Orthopedic Surgery and Rehabilitation
- Elbow and Forearm Trauma Treatment
- Atherosclerosis and Cardiovascular Diseases
- Nanoparticle-Based Drug Delivery
- Bone fractures and treatments
- Plant Stress Responses and Tolerance
- Nerve Injury and Rehabilitation
- Cerebrovascular and Carotid Artery Diseases
- Plant Gene Expression Analysis
- Coronary Interventions and Diagnostics
- Drug Solubulity and Delivery Systems
- Cardiac electrophysiology and arrhythmias
- Melanoma and MAPK Pathways
- Cancer Mechanisms and Therapy
- Ion channel regulation and function
- Light effects on plants
- Peroxisome Proliferator-Activated Receptors
- Advanced Sensor Technologies Research
- Peripheral Nerve Disorders
- Advanced Drug Delivery Systems
- Photosynthetic Processes and Mechanisms
- Cholesterol and Lipid Metabolism
OrthoCarolina
2010-2024
University of Maryland, Baltimore
2024
Northwestern University
2023
Carolinas Medical Center
2010-2023
Wake Forest University
2009-2020
Columbia University
2017-2018
Columbia University Irving Medical Center
2017
Rutgers, The State University of New Jersey
2010-2016
University of Wisconsin–Madison
1990-2014
GlaxoSmithKline (United States)
2012
Auxin and ethylene are key regulators of plant growth development, thus the transcriptional networks that mediate responses to these hormones have been subject intense research. This study dissected hormonal cross talk regulating synthesis flavonols examined their impact on root development. We analyzed effects auxin an precursor roots wild-type hormone-insensitive Arabidopsis (Arabidopsis thaliana) mutants at transcript, protein, metabolite levels high spatial temporal resolution....
Nitric oxide (NO) is considered a key regulator of plant developmental processes and defense, although the mechanism direct targets NO action remain largely unknown. We used phenotypic, cellular, genetic analyses in Arabidopsis thaliana to explore role regulating primary root growth auxin transport. Treatment with donors S-nitroso-N-acetylpenicillamine, sodium nitroprusside, S-nitrosoglutathione reduces cell division, affecting distribution mitotic cells meristem size by reducing number...
We used genetic and molecular approaches to identify mechanisms by which the gaseous plant hormone ethylene reduces lateral root formation enhances polar transport of auxin. Arabidopsis thaliana mutants, aux1, lax3, pin3 pin7, are defective in auxin influx efflux proteins, were less sensitive inhibition stimulation following treatment with precursor 1-aminocyclopropane-1-carboxylic acid (ACC). By contrast, pin2 abcb19 mutants exhibited wild-type ACC responses. indole-3-acetic (IAA) increased...
Gradients of the plant hormone auxin, which depend on its active intercellular transport, are crucial for maintenance root meristematic activity. This directional transport is largely orchestrated by a complex interaction specific influx and efflux carriers that mediate auxin flow into out cells, respectively. Besides these proteins, plant-specific polyphenolic compounds known as flavonols have been shown to act endogenous regulators transport. However, only limited information available how...
A transferred-DNA insertion mutant of Arabidopsis that lacks AKT1 inward-rectifying K+ channel activity in root cells was obtained previously by a reverse-genetic strategy, enabling dissection the K+-uptake apparatus into and non-AKT1 components. Membrane potential measurements demonstrated component wild-type permeability between 55 63% when external [K+] 10 1,000 microM, NH4+ absent. specifically inhibited component, apparently competing for binding sites on transporter(s). This inhibition...
Abstract Two Arabidopsis thaliana ABC transporter genes linked to auxin transport by various previous results were studied in a reverse-genetic fashion. Mutations Multidrug Resistance-Like1 (MDR1) reduced acropetal roots 80% without affecting basipetal transport. Conversely, mutations MDR4 blocked 50% of Developmental and distribution phenotypes associated with these altered flows high-resolution morphometric system confocal microscopy, respectively. Vertically grown mdr1 produced positive...
Abstract Auxin affects the shape of root systems by influencing elongation and branching. Because multidrug resistance (MDR)-like ABC transporters participate in auxin transport, they may be expected to contribute system development. This reverse genetic study Arabidopsis thaliana roots shows that MDR4-mediated basipetal transport did not affect or However, impaired acropetal due mutation MDR1 gene caused 21% nascent lateral arrest their growth remainder elongate 50% more slowly than wild...
To identify gene products that participate in auxin-dependent lateral root formation, a high temporal resolution, genome-wide transcript abundance analysis was performed with auxin-treated Arabidopsis thaliana roots. Data identified 1246 transcripts were consistently regulated by indole-3-acetic acid (IAA), partitioning into 60 clusters distinct response kinetics. We rapidly induced containing auxin-response functional annotations and exhibiting delayed induction linked to cell division...
Significance Lipid-rich plaques in major blood vessels recruit macrophages that further exacerbate the lipid burden and risk of heart attacks or stroke. A local approach to prevent plaque growth has yet be successfully deployed. In this study, we examine how synthetic ligands counteract macrophage atherogenesis de-escalate burden. Using a library sugar-based amphiphilic core-shell layered nanoparticles, demonstrate design principles necessary oxidized uptake suppress scavenger receptor...
Nitric oxide (NO) is a unique reactive nitrogen molecule with an array of signaling functions that modulates plant developmental processes and stress responses. To explore the mechanisms by which NO root development, we used pharmacological approach NO-deficient mutants to unravel role in establishing auxin distribution patterns necessary for stem cell niche homeostasis. Using synthase inhibitor Arabidopsis (Arabidopsis thaliana) biosynthesis (nitric oxide-associated1 [noa1], nitrate...
Angiosperm trees reorient their woody stems by asymmetrically producing a specialized xylem tissue, tension wood, which exerts strong contractile force resulting in negative gravitropism of the stem. Here, we show, Populus trees, that initial gravity perception and response occurs cells through sedimentation starch-filled amyloplasts relocalization auxin transport protein, PIN3. Gibberellic acid treatment stimulates rate wood formation gravibending enhances tissue-specific expression an...
ABSTRACT The structure of the inner and outer surfaces plasmalemma, tonoplast membranes nucleus endoplasmic reticulum have been investigated. plasmalemma probably varies with metabolic state cell, in particular synthesis transport material for cell-wall formation. organization during deposition wall by reverse pinocytosis is shown, evidence presented possible microfibrillar synthetic units arranged as particles on near surface. A clear indication substructure along length microtubules has...
<h3>Importance</h3> Distal radius fractures (DRFs) are common injuries among older adults and can result in substantial disability. Current evidence regarding long-term outcomes is scarce. <h3>Objective</h3> To compare across treatment groups at 24 months with DRFs who participated the WRIST trial. <h3>Design, Setting, Participants</h3> The Wrist Radius Injury Surgical Trial (WRIST) randomized, international, multicenter trial was conducted from April 1, 2012, through December 31, 2016....
Summary ABCB19 of Arabidopsis thaliana (formerly known as MDR1 and PGP19) belongs to the Multidrug Resistance‐like ( MDR ) or B group ATP‐binding cassette (ABC) transporter superfamily, mediates polar auxin transport in stems roots. Here we have investigated role distribution cotyledon development. During embryogenesis, confocal microscopy showed protein be present cotyledons during their main growth phase, but not later. Analysis ProDR5:GFP expression patterns a significantly diminished...
Kinetically assembled nanoparticles are fabricated from an advanced class of bioactive macromolecules that have potential utility in counteracting atherosclerotic plaque development via receptor-level blockage inflammatory cells. In contrast to micellar analogs exhibit poor potency and structural integrity under physiologic conditions, these kinetic nanoparticle assemblies maintain stability demonstrate superior bioactivity mediating oxidized low-density lipoprotein (oxLDL) uptake
Transcriptomic analyses with high temporal resolution provide substantial new insight into hormonal response networks. This study identified the kinetics of genome-wide transcript abundance changes in to elevated levels plant hormone ethylene roots from light-grown Arabidopsis (Arabidopsis thaliana) seedlings, which were overlaid on time-matched developmental changes. Functional annotation clusters transcripts similar patterns revealed rapidly induced known function and more slowly regulated...
To develop a bio-assay that would be able to directly test gastrointestinal and/or dissolution samples determine lipase activity and inhibition by Orlistat.Enzyme assays were performed with porcine pancreatic para-Nitrophenyl Palmitate (pNPP) in pH 8.0 reaction buffer at 37°C. Substrate hydrolysis was monitored absorbance changes 410 nm. The of two Orlistat formulations tested USP II apparatus. Samples HPLC analyzed release profile addition being diluted assayed for inhibitory effect.The...
Plant root development is mediated by the concerted action of auxin and cytokinin phytohormones, with serving as an antagonist transport. Here, we identify AUXIN UP-REGULATED F-BOX PROTEIN1 (AUF1) its potential paralog AUF2 important positive modifiers elongation that tether movements to signaling in Arabidopsis (Arabidopsis thaliana). The AUF1 mRNA level roots strongly up-regulated but not other phytohormones. Whereas auf1 single auf2 double mutant grow normally without exogenous respond...
We previously identified a small molecule, UM101, predicted to bind the substrate-binding groove of p38α mitogen-activated protein kinase (MAPK) near binding site its proinflammatory substrate, kinase-activated (MK)2. UM101 exhibited anti-inflammatory, endothelial-stabilizing, and lung-protective effects. To overcome limited aqueous solubility affinity, we designed an analog GEn-1124, with improved solubility, stability, p38α-binding affinity. Compared GEn-1124 has 18-fold greater affinity...