A5084078812- Photosynthetic Processes and Mechanisms
- Postharvest Quality and Shelf Life Management
- Ubiquitin and proteasome pathways
- Plant Molecular Biology Research
- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Protein Degradation and Inhibitors
- Lipid metabolism and biosynthesis
- Potato Plant Research
- Light effects on plants
- Polymer-Based Agricultural Enhancements
- Plant Reproductive Biology
- Soybean genetics and cultivation
- Plant Gene Expression Analysis
- Plant Physiology and Cultivation Studies
Grand Valley State University
2017-2021
University of Wisconsin–Madison
2008-2012
University of Wisconsin–Eau Claire
2012
Wake Forest University
2011
University of California, Riverside
2007-2008
Zero to Three
2008
Ethylene biosynthesis is directed by a family of 1-aminocyclopropane-1-carboxylic acid (ACC) synthases (ACS) that convert S-adenosyl-l-methionine to the immediate precursor ACC. Members type-2 ACS subfamily are strongly regulated proteolysis with various signals stabilizing proteins increase ethylene production. In Arabidopsis, this turnover mediated ubiquitin/26 S proteasome system, using broad complex/tramtrack/bric-a-brac (BTB) E3 assembled ETHYLENE OVERPRODUCER 1 (ETO1) BTB protein for...
Members of the Bric-a-Brac/Tramtrack/Broad Complex (BTB) family direct selective ubiquitylation proteins following their assembly into Cullin3-based ubiquitin ligases. Here, we describe a subfamily nucleus-localized BTB encoded by LIGHT-RESPONSE BTB1 (LRB1) and LRB2 loci in Arabidopsis (Arabidopsis thaliana) that strongly influences photomorphogenesis. Whereas single lrb1 lrb2 mutants are relatively normal phenotypically, double markedly hypersensitive to red light, but not far-red or blue...
The eer3-1 loss-of-function mutant, which was identified by screening for Arabidopsis mutant seedlings with an enhanced ethylene response, has both increased sensitivity and profound exaggeration of response to when visually assessed, yet exhibits partial insensitivity at the molecular level. mutation represents a conditional allele ethylene-dependent phenotype that results from amino acid substitution in previously uncharacterized prohibitin, AtPHB3, complete loss EER3 function resulting...
Plant root development is mediated by the concerted action of auxin and cytokinin phytohormones, with serving as an antagonist transport. Here, we identify AUXIN UP-REGULATED F-BOX PROTEIN1 (AUF1) its potential paralog AUF2 important positive modifiers elongation that tether movements to signaling in Arabidopsis (Arabidopsis thaliana). The AUF1 mRNA level roots strongly up-regulated but not other phytohormones. Whereas auf1 single auf2 double mutant grow normally without exogenous respond...
eer4 was isolated as an Arabidopsis mutant with extreme response to ethylene in dark-grown seedlings that also found have partial insensitivity at the level of ethylene-dependent gene expression, including ERF1. Subsequent cloning revealed inappropriate stop codon a previously uncharacterized TFIID-interacting transcription factor homologous human TAF12 and yeast TAF61. Genetic pharmacological analysis demonstrated phenotype is strictly dependent seedlings, yet double partially...
Summary An Arabidopsis mutant, eer5‐1 , which has an enhanced ethylene response in etiolated seedlings, including hypersensitivity and extreme exaggeration of to ethylene, was isolated characterized. As with other identified eer mutants, the phenotype correlated failure induce appropriately a subset ethylene‐regulated genes, suggesting that proper ethylene‐responsive gene expression is necessary for resetting pathway. represents mutation causes amino acid substitution previously...
In plants, the small protein related to ubiquitin (RUB) modifies cullin (CUL) proteins in E3 ligases allow for efficient transfer of substrate degradation by 26S proteasome. At molecular level, conjugation RUB individual CUL is transient nature, which aids stability cullins and adaptor proteins. Many changes cellular processes occur within plant upon exposure light, including well-documented However, overall activity between dark- light-grown seedlings has not been assessed plants. order...
Plant growth and development depends on detecting the different wavelengths in electromagnetic spectrum order to perceive light conditions grow accordingly. Phytochrome B, a red far‐red photoreceptor plants, plays an integral role shade avoidance, flowering time, seed germination, de‐etiolation. Proper degradation of this immediate downstream signaling components, Interacting Factors (PIFs), is crucial function these processes occurs via ubiquitin‐proteasome system. The Light Regulating...