A5083963391- Ubiquitin and proteasome pathways
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- Autophagy in Disease and Therapy
- Plant Virus Research Studies
- GABA and Rice Research
- Plant and animal studies
- Endoplasmic Reticulum Stress and Disease
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Plant Genetic and Mutation Studies
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Plant-Microbe Interactions and Immunity
- Plant Diversity and Evolution
- Chromosomal and Genetic Variations
- Rice Cultivation and Yield Improvement
- Child Development and Digital Technology
- Wheat and Barley Genetics and Pathology
- 14-3-3 protein interactions
Ohio University
2015-2024
Henan Polytechnic University
2023
University of Wisconsin–Madison
2008-2013
University of Wisconsin–Eau Claire
2012
Pennsylvania State University
2006-2011
Intercollege
2008
Rice University
1998
Self-incompatibility in flowering plants prevents inbreeding and promotes outcrossing to generate genetic diversity. In Solanaceae, a multiallelic gene, S-locus F-box (SLF), was previously shown encode the pollen determinant self-incompatibility. It postulated that an SLF allelic product specifically detoxifies its non-self S-ribonucleases (S-RNases), products of pistil determinant, inside tubes via ubiquitin-26S-proteasome system, thereby allowing compatible pollinations. However, it...
The covalent attachment of SUMO (small ubiquitin-like modifier) to other intracellular proteins affects a broad range nuclear processes in yeast and animals, including chromatin maintenance, transcription, transport across the envelope, as well protects from ubiquitin addition. Substantial increases SUMOylated upon various stresses have also implicated this modification general stress response. To help understand role(s) SUMOylation plants, we developed stringent method isolate SUMO-protein...
Abstract Petunia inflata S-locus F-box (Pi SLF) is thought to function as a typical protein in ubiquitin-mediated degradation and, along with Skp1, Cullin-1, and Rbx1, could compose an SCF complex mediating the of nonself S-RNase but not self S-RNase. We isolated three P. Skp1s SK1, -2, -3), two Cullin-1s CUL1-C -G), Rbx1 RBX1) cDNAs found that Pi CUL1-G did interact RBX1 none SKs interacted SLF2. also RING-HC protein, Binding Protein1 SBP1), almost identical hybrida SBP1, which interacts...
The emergence of multigene families has been hypothesized as a major contributor to the evolution complex traits and speciation. To help understand how such arose diverged during plant evolution, we examined phylogenetic relationships F-Box (FBX) genes, one largest most polymorphic superfamilies known in kingdom. FBX proteins comprise target recognition subunit SCF-type ubiquitin-protein ligases, where they individually recruit specific substrates for ubiquitylation. Through extensive...
Summary ATP ‐citrate lyases ( ACL ) play critical roles in tumour cell propagation, foetal development and growth, histone acetylation human animals. Here, we report a novel function of death‐mediated pathogen defence responses rice. Using ethyl methanesulphonate EMS mutagenesis map‐based cloning, identified an Oryza sativa ‐A2 mutant allele, termed spotted leaf 30‐1 spl30‐1 ), which A‐to‐T transversion converts Asn at position 343 to Tyr (N343Y), causing recessive mutation that led lesion...
Abstract Petunia inflata possesses S-RNase–based self-incompatibility (SI), which prevents inbreeding and promotes outcrossing. Two polymorphic genes at the S-locus, S-RNase P. S-locus F-box (Pi SLF), determine pistil pollen specificity, respectively. To understand how interactions between Pi SLF result in SI responses, we identified four SLF–like SLFL) used them, along with two previously SLFLs, for comparative studies SLF2. We examined vivo functions of three these SLFLs found that none...
Members of the Bric-a-Brac/Tramtrack/Broad Complex (BTB) family direct selective ubiquitylation proteins following their assembly into Cullin3-based ubiquitin ligases. Here, we describe a subfamily nucleus-localized BTB encoded by LIGHT-RESPONSE BTB1 (LRB1) and LRB2 loci in Arabidopsis (Arabidopsis thaliana) that strongly influences photomorphogenesis. Whereas single lrb1 lrb2 mutants are relatively normal phenotypically, double markedly hypersensitive to red light, but not far-red or blue...
Summary S‐RNase‐based self‐incompatibility has been identified in three flowering plant families, including the Solanaceae, and this self/non‐self recognition mechanism between pollen pistil is controlled by two polymorphic genes at S ‐locus, S‐RNase S‐locus F‐box ( SLF ). produced taken up tubes a non‐ S‐ haplotype‐specific manner. How an allelic product of interacts with self non‐self S‐RNases to result growth inhibition not completely understood. One model predicts that targets for...
SUMMARY Functional studies of the ubiquitin–26S proteasome system (UPS) have demonstrated that virtually all aspects plant's life involve UPS‐mediated turnover abnormal or short‐lived proteins. However, role UPS during development, including in seeds and fruits, remains to be determined detail, although mutants several its core elements are known embryonically lethal. Unfortunately, early termination embryogenesis limits possibility characterize activities reproductive organs. Given both...
Retrograde signaling modulates the expression of nuclear genome-encoded organelle proteins to adjust function in response environmental cues. MULTIPLE ORGANELLAR RNA EDITING FACTOR 2 (MORF2) was initially recognized as a plastidial RNA-editing factor but recently shown interact with GUN1. Given central role GUN1 chloroplast retrograde and unviable phenotype morf2 mutants that is inconsistent many viable involved editing, we hypothesized MORF2 has functions either dosage dependent or beyond...
Comparisons within expanding sequence databases have revealed a dynamic interplay among genomic and epigenomic forces in driving plant evolution. Such are especially obvious the F-Box (FBX) superfamily, one of largest most polymorphic gene families land plants, where its frequent lineage-specific expansions contractions provide an excellent model to assess how genetic variation impacted function before after speciation. Previous phylogenetic comparisons based on orthology, diversity,...
Summary DNA methylation plays crucial roles in cellular development and stress responses through gene regulation genome stability control. Precise of DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2), the de novo Arabidopsis methyltransferase, is to maintain homeostasis ensure integrity. Compared with extensive studies on DRM2 targeting mechanisms, little information known regarding quality control itself. Here, we conducted yeast two‐hybrid screen assay identified an E3 ligase, COP9 INTERACTING...
With the increasing economic growth in developing nations, soil heavy metal pollution has become a growing concern. Monitoring concentration through remote sensing is crucial for safeguarding ecological environment. However, current indoor spectral measurement method limitations, such as discrete sampling space and weak characteristics of metals, leading to poor robustness inversion models. This study presents novel approach address these challenges by incorporating spatial feature sources...
Abstract Plastid biogenesis and the coordination of plastid nuclear genome expression through anterograde retrograde signaling are essential for plant development. GENOMES UNCOUPLED1 (GUN1) plays a central role in during early The putative function GUN1 has been extensively studied, but its molecular remains controversial. Here, we evaluate published transcriptome data generate our own from gun1 mutants grown under relevant conditions to show that editing splicing not GUN1-dependent...
Plastid biogenesis and the coordination of plastid nuclear genome expression through anterograde retrograde signaling are essential for plant development. GENOMES UNCOUPLED1 (GUN1) plays a central role in during early The putative function GUN1 has been extensively studied, but its molecular remains controversial. Here, we evaluate published transcriptome data generate our own from gun1 mutants grown under signaling-relevant conditions to show that editing splicing not relevant...
Pistils of flowering plants possessing self-incompatibility (SI) can distinguish between self and non-self pollen, only allow pollen to effect fertilization. For Petunia inflata, the S-RNase gene encodes pistil specificity multiple S-locus F-box (SLF) genes encode specificity. Each SLF produced in interacts with a subset S-RNases mediate their ubiquitination degradation by 26S proteasome.S-locus has been proposed function as component conventional SCF (SKP1-CULLIN-F-box protein) complex,...
: Genome amplification and sequence divergence provides raw materials to allow organismal adaptation. This is exemplified by the large expansion of ubiquitin-26S proteasome system (UPS) in land plants, which primarily rely on intracellular signaling biochemical metabolism combat biotic abiotic stresses. While a handful functional genomic studies have demonstrated adaptive role UPS plant growth development, many members remain unknown. In this work, we applied comparative study address at...
Summary Ubiquitin (Ub) and Ub‐like proteins, collectively forming the ubiquiton family, regulate nearly all aspects of cellular processes via post‐translational modifications. Studies devoted to specific members suggested a large expansion this family in plants; however, lack systematic analysis hinders comparison individual at both evolutionary history functional divergence levels, which may provide new insight into biological functions. In work, we first retrieved total 5856 17 known...