A5024275400- Plant Molecular Biology Research
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Plant Virus Research Studies
- Remote Sensing in Agriculture
- Plant nutrient uptake and metabolism
- Greenhouse Technology and Climate Control
- Photosynthetic Processes and Mechanisms
- Crop Yield and Soil Fertility
- Leaf Properties and Growth Measurement
- Chromosomal and Genetic Variations
- Polysaccharides and Plant Cell Walls
- Phytoplasmas and Hemiptera pathogens
- Spectroscopy and Chemometric Analyses
- Plant Water Relations and Carbon Dynamics
- Plant biochemistry and biosynthesis
- Transgenic Plants and Applications
- Ubiquitin and proteasome pathways
- Molecular Biology Techniques and Applications
- Biofuel production and bioconversion
- Fungal Biology and Applications
- Advanced Proteomics Techniques and Applications
- Gene expression and cancer classification
- Potato Plant Research
Ghent University
2011-2025
VIB-UGent Center for Plant Systems Biology
2011-2024
Vlaams Instituut voor Biotechnologie
2000-2015
Ghent University Hospital
2003
Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes which compatible with both approaches. The GSTs were carefully selected ensure that each them shared no significant similarity any...
Plant-parasitic nematodes are destructive plant pathogens that cause significant yield losses. They induce highly specialized feeding sites (NFS) in infected roots from which they withdraw nutrients. In order to establish these NFS, it is thought the manipulate molecular and physiological pathways of their hosts. Evidence accumulating signalling molecule auxin involved initiation development sedentary plant-parasitic nematodes. Intercellular transport essential for various aspects growth...
The plant hormone auxin and its directional transport are known to play a crucial role in defining the embryonic axis subsequent development of body plan. Although PIN efflux transporters has been clearly assigned during shoot root specification, influx carriers AUX1 LIKE-AUX1 (LAX) proteins is not well established. Here, we used chemical genetic tools on Brassica napus microspore-derived embryos Arabidopsis thaliana zygotic embryos, demonstrate that AUX1, LAX1 LAX2 required for both pole...
Plant bZIP group I transcription factors have been reported mainly for their role during vascular development and osmosensory responses. Interestingly, bZIP29 has identified in a cell cycle interactome, indicating additional functions of plant development. Here, was functionally characterized to study its It is not present tissue but specifically expressed proliferative tissues. Genome-wide mapping target genes confirmed stress responses, also specific binding several core involved wall...
Using synchronized tobacco Bright Yellow-2 cells and cDNA-amplified fragment length polymorphism-based genomewide expression analysis, we built a comprehensive collection of plant cell cycle-modulated genes. Approximately 1,340 periodically expressed genes were identified, including known cycle control as well numerous unique candidate regulatory A number plant-specific found to be modulated. Other transcript tags derived from unknown showing homology cycle-regulatory other organisms. Many...
Hyperspectral imaging is a promising tool for non-destructive phenotyping of plant physiological traits, which has been transferred from remote to proximal sensing applications, and manual laboratory setups automated platforms. Due the higher resolution in sensing, illumination variation geometry result increased non-biological spectra that may mask subtle biological differences. Here, better understanding spectral measurements their application study drought, developmental diurnal responses...
Abstract Crop improvement goals for maize ( Zea mays L.) involve the targeted optimization of various organs, making it crucial to understand developmental characteristics and gene expression patterns during organ development in response environmental stresses such as drought. In this study, we investigated leaves internodes at both macroscopic cellular level, identified a shared fundamental growth design with distinct timing between two organs. By transcriptome profiling zones different...
SUMMARY To analyse the parasitic behaviour of plant‐parasitic nematode Heterodera schachtii , proteins secreted by this were purified and separated two‐dimensional gel electrophoresis. Mass spectrometric analysis identified one spots as a pectate lyase (EC 4.2.2.2). The corresponding gene was cloned from cDNA library using primers derived peptide tag. A second based on similarity to known lyases related cyst nematodes. predicted are only 29% identical. Despite low homology, have similar...
Drought at flowering and grain filling greatly reduces maize (Zea mays) yield. Climate change is causing earlier longer-lasting periods of drought, which affect the growth multiple organs throughout development. To study how long water deficit impact dynamic nature growth, to determine these relate reproductive we employed a high-throughput phenotyping platform featuring precise irrigation, imaging systems, image-based biomass estimations. Prolonged drought resulted in reduction rate...
Thermography is a popular tool to assess plant water-use behavior, as temperature influenced by transpiration rate, and commonly used in field experiments detect water deficit. Its application indoor automated phenotyping platforms still limited mainly focuses on differences between genotypes or treatments, instead of estimating stomatal conductance rate. In this study, the transferability thermography analysis protocols from greenhouse was evaluated. addition, added value combining thermal...
Abstract Plant-parasitic nematodes secrete a plethora of enzymes to degrade polysaccharides the recalcitrant plant cell wall. Here we report on presence putative endo-1,4-β-galactosidase (EC 3.2.1.89) in cyst genus Heterodera. This enzyme hydrolyses β-1,4-galactan hairy regions pectin and our knowledge it is first this class animals. The gene was cloned from H. schachtii subjected detailed molecular characterisation. deduced protein contains signal peptide for secretion, being agreement with...
ABSTRACT Drought is one of the most devastating causes yield losses in crops like maize, and anticipated increases severity duration drought spells due to climate change pose an imminent threat agricultural productivity. To understand response, phenotypic molecular studies are typically performed at a given time point after onset, representing steady‐state adaptation response. Because growth dynamic process, we monitored response with high temporal resolution examined cellular transcriptomic...
The ability to tag proteins has boosted the emergence of generic molecular methods for protein functional analysis. Fluorescent tags are used visualize localization, and affinity enable mapping interactions by, example, tandem purification or chromatin immunoprecipitation. To apply these widely techniques on a single transgenic plant line, we developed multifunctional tag, named GSyellow, which combines streptavidin-binding peptide with citrine yellow fluorescent protein. We demonstrated...
The activity of the Arabidopsis thaliana cyclin-dependent kinase AtCDKA;1 is important throughout G(1)/S and G(2)/M transitions guarantees progression cell cycle. Inhibitor studies have shown that activation cycle for development nematode feeding sites. aim this study was to silence expression gene in sites interfere with their development. Therefore, sense antisense constructs were made fused a nematode-inducible promoter which activated at an earlier time point than AtCDKA;1. Two...
Abstract Background Thermography is a popular tool to assess plant water use behavior, as temperature influenced by transpiration rate, and commonly used in field experiments detect drought stress. Its application indoor automated phenotyping platforms still limited mainly focuses on differences between genotypes or treatments, instead of estimating stomatal conductance rate. In this study, the transferability thermography analysis protocols from greenhouse was evaluated. addition, added...