A5086414502- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Melanoma and MAPK Pathways
- Mycorrhizal Fungi and Plant Interactions
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- Plant and animal studies
- Advanced Proteomics Techniques and Applications
- Plant Parasitism and Resistance
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Protein Kinase Regulation and GTPase Signaling
- Plant Stress Responses and Tolerance
- Biochemical and Structural Characterization
- Peptidase Inhibition and Analysis
- Cell death mechanisms and regulation
- Wheat and Barley Genetics and Pathology
- Advancements in Semiconductor Devices and Circuit Design
- Phytase and its Applications
- RNA modifications and cancer
- Scientific Research and Discoveries
- Cancer Mechanisms and Therapy
VIB-UGent Center for Medical Biotechnology
2014-2021
Ghent University
2010-2021
VIB-UGent Center for Plant Systems Biology
2010-2021
Vlaams Instituut voor Biotechnologie
2009-2016
Volgograd Institute of Business
2016
University of Bologna
2011
University College Ghent
2011
Decades ago, the importance of cytokinins (CKs) during Rhodococcus fascians pathology had been acknowledged, and an isopentenyltransferase gene characterized in fas operon linear virulence plasmid, but hitherto, no specific CK(s) could be associated with virulence. We show that CK receptors AHK3 AHK4 Arabidopsis thaliana are essential for symptom development, perception machinery is induced upon infection, underlining its central role symptomatology. Three classical CKs [isopentenyladenine,...
Roots explore the soil for water and nutrients through continuous production of lateral roots. Lateral roots are formed at regular distances in a steadily elongating organ, but how future sites root formation become established is not yet understood. Here, we identified C-TERMINALLY ENCODED PEPTIDE 5 (CEP5) as novel, auxin-repressed phloem pole-expressed signal assisting In addition, based on genetic expression data, found evidence involvement its proposed receptor, XYLEM INTERMIXED WITH...
The phytopathogenic actinomycete Rhodococcus fascians D188 relies mainly on the linear plasmid-encoded fas operon for its virulence. bacteria secrete six cytokinin bases that synergistically redirect developmental program of plant to stimulate proliferation young shoot tissue, thus establishing a leafy gall as niche. A yeast-based bioassay combined with profiling bacterial mutants revealed is essential enhanced production isopentenyladenine, trans-zeatin, cis-zeatin, and 2-methylthio...
Abstract Plants respond to mild warm temperature conditions by increased elongation growth of organs enhance cooling capacity, in a process called thermomorphogenesis. To this date, the regulation thermomorphogenesis has been exclusively shown intersect with light signalling pathways. identify regulators that are conserved flowering plants, we map changes protein phosphorylation both dicots and monocots exposed temperature. We MITOGEN-ACTIVATED PROTEIN KINASE KINASE4 (MAP4K4)/TARGET OF...
Here, we apply the COmbined FRActional DIagonal Chromatography (COFRADIC) technology to enrich for ubiquitinated peptides and identify sites of ubiquitination by mass spectrometry. Our bypasses need overexpress tagged variants ubiquitin use sequence-biased antibodies recognizing remnants. In brief, all protein primary amino groups are blocked chemical acetylation, after which chains proteolytically specifically removed catalytic core domain USP2 deubiquitinase (USP2cc). Because USP2cc...
Leafy gall syndrome is the consequence of modified plant development in response to a mixture cytokinins secreted by biotrophic actinomycete Rhodococcus fascians. The similarity induced symptoms with phenotype mutants defective strigolactone biosynthesis and signalling prompted an evaluation involvement strigolactones this pathology. All tested strigolactone-related Arabidopsis thaliana were hypersensitive R. Moreover, treatment synthetic GR24 carotenoid cleavage dioxygenase inhibitor D2...
Protein phosphorylation is one of the most common post-translational modifications (PTMs), which can regulate protein activity and localization as well protein-protein interactions in numerous cellular processes. Phosphopeptide enrichment techniques enable plant researchers to acquire insight into phosphorylation-controlled signaling networks various species. Most phosphoproteome analyses samples still involve stable isotope labeling, peptide fractionation, demand a lot mass spectrometry...
CEP5 promotes abiotic stress tolerance and AUX/IAA stability
In plants, the generation of new cell types and tissues depends on coordinated oriented formative divisions. The plasma membrane-localized receptor kinase ARABIDOPSIS CRINKLY 4 (ACR4) is part a mechanism controlling divisions in Arabidopsis root. Despite its important role plant development, very little known about molecular with which ACR4 affiliated network interactions. Here, we used various complementary proteomic approaches to identify ACR4-interacting protein candidates that are likely...
The V600E missense mutation in B-Raf kinase leads to an anomalous regulation of the MAPK pathway, uncontrolled cell proliferation, and initiation tumorigenesis. While ATP-competitive inhibitors block pathway mutant cells, they induce conformational changes wild-type domain leading heterodimerization with C-Raf causing a paradoxical hyperactivation pathway. A new class (paradox breakers) has been developed that inhibit B-RafV600E activity without agonistically affecting cells. In this study,...
Strigolactones are plant metabolites that act as phytohormones and rhizosphere signals. Whereas most research on unraveling the action mechanisms of strigolactones is focused shoots, we investigated proteome adaptation during strigolactone signaling in roots Arabidopsis thaliana. Through large-scale, time-resolved, quantitative proteomics, impact analog rac-GR24 was elucidated root wild type mutant more axillary growth 2 (max2). Our study revealed a clear MAX2-dependent response: an increase...
Abstract The phytopathogenic actinomycete Rhodococcus fascians drives its host to form a nutrient-rich niche by secreting mixture of cytokinins that triggers plant cell division and shoot formation. discrepancy between the relatively low amount secreted severe impact R. infection on development has puzzled researchers for long time. Polyamine transcript profiling wild-type cytokinin receptor mutant plants revealed bacterial directly stimulated biosynthesis putrescine activating arginine...
Wheat is a cereal grain and one of the world's major food crops. Recent advances in wheat genome sequencing are by now facilitating its genomic proteomic analyses. However, little known about possible difference total protein levels hexaploid versus tetraploid cultivars, also knowledge on phosphorylated proteins still limited. Here, we performed detailed analysis proteome seedling leaves from two cultivars (T. aestivum L. Pavon 76 USU-Apogee) (Triticum turgidum ssp. durum cv. Senatore...
The biotrophic phytopathogen Rhodococcus fascians has a profound impact on plant development, mainly through its principal virulence factors, mix of synergistically acting cytokinins that induce shoot formation. Expression profiling marker genes for several auxin biosynthesis routes and mutant analysis demonstrated the bacterial stimulate plants via specific targeting indole-3-pyruvic acid (IPA) pathway, resulting in enhanced signaling infected tissues. double tryptophan aminotransferase 1-1...
GENERAL COMMENTARY article Front. Plant Sci., 09 April 2015Sec. Proteomics and Protein Structural Biology Volume 6 - 2015 | https://doi.org/10.3389/fpls.2015.00224
Likely due to conformational rearrangements, small molecule inhibitors may stabilize the active conformation of protein kinases and paradoxically promote tumorigenesis. We combined limited proteolysis with stable isotope labeling MS monitor changes upon binding molecules. Applying this method human serine/threonine kinase B-Raf, frequently mutated in cancer, we found that ATP or its nonhydrolyzable analogue AMP-PNP, but not ADP, stabilized structure both B-RafWT B-RafV600E. The...