A5048842264- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Identification and Quantification in Food
- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Plant Pathogens and Fungal Diseases
- Dysphagia Assessment and Management
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Polyamine Metabolism and Applications
- Plant-Microbe Interactions and Immunity
- Amino Acid Enzymes and Metabolism
- Dermatology and Skin Diseases
- Mycorrhizal Fungi and Plant Interactions
- Mycobacterium research and diagnosis
- Enzyme-mediated dye degradation
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Fungal Biology and Applications
- Biofuel production and bioconversion
- Plant Reproductive Biology
University of Helsinki
2016-2025
Helsinki Institute of Physics
2009-2023
Czech Academy of Sciences, Institute of Biotechnology
2000-2023
Helsinki Art Museum
2020-2022
"Dunarea de Jos" University of Galati
2022
Emory University
2021
Norwegian University of Life Sciences
2021
Université de Montpellier
2020
Inserm
2020
Helsinki University Hospital
2020
In the course of Parkinson's disease (PD), enteric nervous system (ENS) and parasympathetic nerves are amongst structures earliest most frequently affected by alpha-synuclein pathology. Accordingly, gastrointestinal dysfunction, in particular constipation, is an important non-motor symptom PD often precedes onset motor symptoms years. Recent research has shown that intestinal microbiota interact with autonomic central via diverse pathways including ENS vagal nerve. The gut microbiome not...
Rapidly declining biodiversity may be a contributing factor to another global megatrend—the rapidly increasing prevalence of allergies and other chronic inflammatory diseases among urban populations worldwide. According the “biodiversity hypothesis,” reduced contact people with natural environmental features adversely affect human commensal microbiota its immunomodulatory capacity. Analyzing atopic sensitization (i.e., allergic disposition) in random sample adolescents living heterogeneous...
To unravel the biological function of widely used probiotic bacterium Lactobacillus rhamnosus GG, we compared its 3.0-Mbp genome sequence with similarly sized L. LC705, an adjunct starter culture exhibiting reduced binding to mucus. Both genomes demonstrated high identity and synteny. However, for both strains, genomic islands, 5 in GG 4 punctuated colinearity. A significant number strain-specific genes were predicted these islands (80 72 LC705). The GG-specific included coding bacteriophage...
Abstract Background Composting is an aerobic microbiological process that facilitated by bacteria and fungi. also a method to produce fertilizer or soil conditioner. Tightened EU legislation now requires treatment of the continuously growing quantities organic municipal waste before final disposal. However, some full-scale composting plants experience difficulties with efficiency biowaste degradation emission noxious odours. In this study we examine bacterial species richness community...
Abstract Background Previous studies have reported that gut microbiota, permeability, short-chain fatty acids (SCFAs), and inflammation are altered in Parkinson’s disease (PD), but how these factors linked they contribute to processes symptoms remains uncertain. This study sought compare identify associations among PD patients controls elucidate their interrelations links clinical manifestations of PD. Methods Stool plasma samples data were collected from 55 56 controls. Levels stool SCFAs...
Several publications have described differences in cross-sectional comparisons of gut microbiota between patients with Parkinson's disease and control subjects, considerable variability the reported differentially abundant taxa. The temporal stability such alterations their relationship to progression not been previously studied a high-throughput sequencing based approach.We collected clinical data stool samples from 64 subjects twice, on average 2·25 years apart. Disease was evaluated...
Abstract Background A growing amount of scientific evidence suggests that microbes are involved in the aetiology irritable bowel syndrome (IBS), and gastrointestinal (GI) microbiota individuals suffering from diarrhoea-predominant IBS (IBS-D) is distinguishable other IBS-subtypes. In our study, GI IBS-D patients was evaluated compared with healthy controls (HC) by using a high-resolution sequencing method. The method allowed microbial community analysis on all levels genomic guanine plus...
We spend most of our lives in indoor environments and are exposed to microbes present these environments. Hence, knowledge about this exposure is important for understanding how it impacts on human health. However, the bacterial flora has been only fragmentarily explored mostly using culture methods. The application molecular methods previously utilised other resulted a substantial increase awareness microbial diversity.The composition dynamics dust were investigated two buildings over...
Yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating fungal disease threatening much of global wheat production. Race-specific resistance (R)-genes are used to control rust diseases, but the rapid emergence virulent Pst races has prompted search for more durable resistance. Here, we report cloning Yr15, broad-spectrum R-gene derived from wild emmer wheat, which encodes putative kinase-pseudokinase protein, designated as tandem kinase 1, comprising unique...
Silver birch (Betula pendula) is a pioneer boreal tree that can be induced to flower within 1 year. Its rapid life cycle, small (440-Mb) genome, and advanced germplasm resources make an attractive model for forest biotechnology. We assembled chromosomally anchored the nuclear genome of inbred B. pendula individual. Gene duplicates from paleohexaploid event were enriched transcriptional regulation, whereas tandem overrepresented by environmental responses. Population resequencing 80...
Previous studies have reported that chromosome synteny in Lepidoptera has been well conserved, yet the number of haploid chromosomes varies widely from 5 to 223. Here we report genome (393 Mb) Glanville fritillary butterfly (Melitaea cinxia; Nymphalidae), a recognized model species metapopulation biology and eco-evolutionary research, which putative ancestral karyotype n=31. Using phylogenetic analyses Nymphalidae other Lepidoptera, combined with orthologue-level comparisons chromosomes,...
Lactobacillus rhamnosus is a lactic acid bacterium that found in large variety of ecological habitats, including artisanal and industrial dairy products, the oral cavity, intestinal tract or vagina. To gain insights into genetic complexity versatility species L. rhamnosus, we examined genomes phenotypes 100 strains isolated from diverse sources. The were mapped onto GG reference genome. These phenotypically characterized for wide range metabolic, antagonistic, signalling functional...
The gut microbiome and its metabolites can impact brain health are altered in Parkinson's disease (PD) patients. It has been recently demonstrated that PD patients have reduced fecal levels of the potent epigenetic modulator butyrate bacterial producers.Here, we investigate whether changes associated related to symptoms markers leucocytes neurons.Stool, whole blood samples, clinical data were collected from 55 controls. We performed DNA methylation analysis on samples analyzed results...
Toxic and non-toxic cyanobacterial strains from Anabaena, Aphanizomenon, Calothrix, Cylindrospermum, Nostoc, Microcystis, Planktothrix (Oscillatoria agardhii), Oscillatoria Synechococcus genera were examined by RFLP of PCR-amplified 16S rRNA genes gene sequencing. With both methods, high similarity was found among planktic, anatoxin-a-producing Anabaena microcystin-producing different geographical origins. The respective sequence similarities 99.9-100%, 94.2-99.9% 99.3-100%. Thus the...
Abstract Background Plants growing in their natural habitat represent a valuable resource for elucidating mechanisms of acclimation to environmental constraints. Populus euphratica is salt-tolerant tree species saline semi-arid areas. To identify genes involved abiotic stress responses under conditions we constructed several normalized and subtracted cDNA libraries from control, stress-exposed desert-grown P. trees. In addition, identified metabolites Results About 14,000 expressed sequence...
Ageing has been suggested to cause changes in the intestinal microbial community. In present study, microbiota of a previously well-defined group elderly subjects aged between 70 and 85 years, both non-steroidal anti-inflammatory drugs (NSAID) users ( n 9) non-users 9), were further compared with young adults 14) mean age 28 by two DNA-based techniques: percentage guanine+cytosine (%G+C) profiling 16S rDNA sequencing. Remarkable described methods: significant reduction overall numbers...
In recent years increasing attention has been given to the potential health effects of fungal exposure in indoor environments. We used large-scale sequencing internal transcribed spacer region (ITS) nuclear ribosomal DNA describe mycoflora two office buildings over four seasons. was complemented by cultivation, ergosterol determination, and quantitative PCR analyses. Sequences 1,339 clones were clustered into 394 nonredundant operational taxonomical units containing sequences from 18...
Thirteen bacterial isolates from lake sediment, capable of degrading cyanobacterial hepatotoxins microcystins and nodularin, were characterized by phenotypic, genetic genomic approaches. Cells these Gram-negative, motile means a single polar flagellum, oxidase-positive, weakly catalase-positive rod-shaped. According to phenotypic characteristics (carbon utilization, fatty acid enzyme activity profiles), the G+C content DNA (66·1–68·0 mol%) 16S rRNA gene sequence analysis (98·9–100 %...