A5004957175- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Genetics and Plant Breeding
- Yeasts and Rust Fungi Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Plant-Microbe Interactions and Immunity
- Plant pathogens and resistance mechanisms
- Advances in Cucurbitaceae Research
- Plant nutrient uptake and metabolism
- Agricultural Practices and Plant Genetics
- Berry genetics and cultivation research
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Resistance
- Plant Pathogenic Bacteria Studies
- Genetic diversity and population structure
- Mycotoxins in Agriculture and Food
- Genetic and Environmental Crop Studies
- Plant Reproductive Biology
- Insect-Plant Interactions and Control
- Plant Surface Properties and Treatments
- Rice Cultivation and Yield Improvement
- Ecology and Vegetation Dynamics Studies
- Plant Micronutrient Interactions and Effects
University of Haifa
2013-2025
Tel Aviv University
2015-2024
Kansas State University
2014
Western Regional Research Center
2009
University of California, Davis
2009
Washington State University
2009
Guizhou University
2009
Carmel (Israel)
2008
Wheat (Triticum spp.) is one of the founder crops that likely drove Neolithic transition to sedentary agrarian societies in Fertile Crescent more than 10,000 years ago. Identifying genetic modifications underlying wheat's domestication requires knowledge about genome its allo-tetraploid progenitor, wild emmer (T. turgidum ssp. dicoccoides). We report a 10.1-gigabase assembly 14 chromosomes tetraploid wheat, as well analyses gene content, architecture, and diversity. With this fully assembled...
Stripe rust is a devastating fungal disease that afflicts wheat in many regions of the world. New races Puccinia striiformis , pathogen responsible for this disease, have overcome most known race-specific resistance genes. We report map-based cloning gene Yr36 ( WKS1 ), which confers to broad spectrum stripe at relatively high temperatures (25° 35°C). This includes kinase and putative START lipid-binding domain. Five independent mutations transgenic complementation confirmed both domains are...
Yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating fungal disease threatening much of global wheat production. Race-specific resistance (R)-genes are used to control rust diseases, but the rapid emergence virulent Pst races has prompted search for more durable resistance. Here, we report cloning Yr15, broad-spectrum R-gene derived from wild emmer wheat, which encodes putative kinase-pseudokinase protein, designated as tandem kinase 1, comprising unique...
SUMMARY Aegilops is a close relative of wheat ( Triticum spp.), and species in the section Sitopsis represent rich reservoir genetic diversity for improvement wheat. To understand their advance utilization, we produced whole‐genome assemblies longissima speltoides . Whole‐genome comparative analysis, along with recently sequenced sharonensis genome, showed that Ae. genomes are highly similar most closely related to D subgenome. By contrast, genome more B Haplotype block analysis supported...
Gene cloning in repeat-rich polyploid genomes remains challenging. Here we describe a strategy for overcoming major bottlenecks the of powdery mildew (Pm) resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat (WEW). A conventional positional approach was not effective due to suppressed recombination. Chromosome sorting compromised by insufficient purity. physical map, constructed assembling Oxford Nanopore Technology (ONT) long-read genome sequences, revealed rapidly...
Wheat stem rust, a devastating disease of wheat and barley caused by the fungal pathogen Puccinia graminis f. sp. tritici, was largely eradicated in Western Europe during mid-to-late twentieth century. However, isolated outbreaks have occurred recent years. Here we investigate whether lack resistance modern European varieties, increased presence its alternate host barberry changes climatic conditions could be facilitating resurgence. We report first rust occurrence United Kingdom nearly 60...
ABSTRACT Our aim was to elucidate mechanisms underlying nitrogen (N)‐deficiency tolerance in bread wheat (cultivar Ruta), conferred by a wild emmer QTL (WEW; IL99). We hypothesised that the IL99 is driven enhanced N‐uptake through modification of root system architecture (RSA) underscored transcriptome modifications. Severe N‐deficiency (0.1 N for 26 days) triggered significantly higher plasticity compared Ruta modifying 16 RSA traits; nine which were IL99‐specific. The change growth...
We report a group of TRIMs (terminal-repeat retrotransposons in miniature), which are small nonautonomous retrotransposons. These elements, named Cassandra , universally carry conserved 5S RNA sequences and associated polymerase (pol) III promoters terminators their long terminal repeats (LTRs). They were found all vascular plants investigated. Uniquely for LTR retrotransposons, produces noncapped, polyadenylated transcripts from the pol promoter. Capped, read-through containing can also be...
Nucleotide-binding leucine-rich repeat (NLR) disease resistance genes typically confer against races of a single pathogen. Here, we report that Yr87/Lr85, an NLR gene from Aegilops sharonensis and longissima, confers both P. striiformis tritici (Pst) Puccinia triticina (Pt) cause stripe leaf rust, respectively. Yr87/Lr85 Pst Pt in wheat introgression as well transgenic lines. Comparative analysis the cloned Triticeae shows contains two distinct LRR domains is only found Ae. longissima....
The wheat genome sequence is an essential tool for advanced genomic research and improvements. generation of a high-quality challenging due to its complex 17 Gb polyploid genome. To overcome these difficulties, sequencing through the construction BAC-based physical maps individual chromosomes employed by genomics community. Here, we present first comprehensive map chromosome 1BS, illustrate unique gene space organization evolution. Fingerprinted BAC clones were assembled into 57 long...
The objectives of this study were to identify genetic loci in the bread wheat genome that would influence yield stability and quality under water stress, accessions can be recommended for cultivation dry hot regions. We performed a genome-wide association (GWAS) using panel 232 spanning diverse ecogeographic Plants evaluated Israeli Northern Negev, two environments: water-limited (D; 250 mm) well-watered (W; 450 conditions; they genotyped with ~71,500 SNPs derived from exome capture...
SUMMARY In this study, we explore the diversity and its distribution along wheat leaf rust resistance protein LR10 three‐dimensional structure. Lr10 is a gene encoding coiled coil–nucleotide‐binding site–leucine‐rich repeat (CC–NBS–LRR) class of protein. was cloned sequenced from 58 accessions representing diverse habitats wild emmer in Israel. Nucleotide very high relative to other genes ( π = 0.029). The CC domain found be most subject positive selection. Superimposition on structure...
Many accessions of the wheat wild relative Sharon goatgrass ( Aegilops sharonensis Eig., AES ) are resistant to African races stem rust pathogen (i.e., Ug99 group races), which currently threaten production worldwide. A procedure was designed introgress respective resistances specific bread genomes by producing plants homozygous for and B hemizygous D S sh or genomes. In these genotypes, lack Ph1 allele, homeologous pairing expected mainly between chromosomes genomes, respectively. An...
The bird cherry-oat aphid (Rhopalosiphum padi) is one of the most destructive insect pests in wheat production. To reduce damage, plants have evolved various chemical and physical defense mechanisms. Although these mechanisms been frequently reported, much less known about their effectiveness. tetraploid wild emmer (WEW; Triticum turgidum ssp. dicoccoides), progenitors domesticated wheat, possesses untapped resources from its numerous desirable traits, including resistance. goal this...
We identified two novel wheat stem rust resistance genes, Sr-1644-1Sh and Sr-1644-5Sh in Aegilops sharonensis that are effective against widely virulent African races of the pathogen. Stem is one most important diseases world. When single (Sr) genes deployed wheat, they often rapidly overcome by To this end, we initiated a search for sources diverse relatives wild goatgrass species sharonesis (Sharon goatgrass) as rich reservoir to rust. The objectives study were discover map Sr Ae. explore...
Drought is the major environmental factor limiting wheat production worldwide. Developing novel cultivars with greater drought tolerance most viable solution to ensure sustainable agricultural and alleviating threats food-security. Here we established a core-collection of landraces modern durum (WheatME, n = 36), from Middle East region (Jordan, Palestine Israel) aiming at unlocking genetic morpho-physiological adaptation semi-arid environment conditions. Interestingly, analysis WheatME...
Central to the diversity of wheat products was origin hexaploid bread wheat, which added D-genome Aegilops tauschii tetraploid giving rise superior dough properties in leavened breads. The polyploidization, however, imposed a genetic bottleneck, with only limited introduced D-subgenome. To understand variants for quality, we sequenced 273 accessions spanning known Ae. tauschii. We discovered 45 haplotypes Glu-D1, major determinant relative two predominant wheat. allele 2 + 12 found Lineage...
Stripe rust is a devastating disease in wheat that causes substantial yield loss around the world. The most effective strategy for mitigating to develop resistant cultivars. wild relatives of are good sources resistance fungal pathogens. Here, we used genome-wide association study (GWAS) identify loci associated with stripe (causal agent: Puccinia striiformis f. sp. tritici) emmer (Triticum dicoccoides) at seedling stage, greenhouse, and adult plant field. We found two major contributing our...