A5068553048- Plant-Microbe Interactions and Immunity
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Plant pathogens and resistance mechanisms
- Plant Pathogenic Bacteria Studies
- Lipid metabolism and biosynthesis
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Proteins in Food Systems
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Resistance
- Plant Virus Research Studies
- Membrane Separation Technologies
- Electrospun Nanofibers in Biomedical Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Hydrolysis and Bioactive Peptides
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Cellular transport and secretion
- Platelet Disorders and Treatments
- Plant Pathogens and Fungal Diseases
- Biochemical and Structural Characterization
- Aerosol Filtration and Electrostatic Precipitation
- Plant and Fungal Interactions Research
Sainsbury Laboratory
2013-2024
University of East Anglia
2007-2023
Norwich Research Park
1999-2023
Consejo Superior de Investigaciones Científicas
2009-2012
John Innes Centre
1999-2001
Universidad de Sevilla
1992-2000
Instituto de la Grasa
1994-1996
Abstract Sequence assembly of large and repeat-rich plant genomes has been challenging, requiring substantial computational resources often several complementary sequence genome mapping approaches. The recent development fast accurate long-read sequencing by circular consensus (CCS) on the PacBio platform may greatly increase scope pan-genome projects. Here, we compare current platforms regarding their ability to rapidly generate contiguous assemblies in studies barley (Hordeum vulgare)....
Abstract The wild relatives and progenitors of wheat have been widely used as sources disease resistance ( R ) genes. Molecular identification characterization these genes facilitates their manipulation tracking in breeding programmes. Here, we develop a reference-quality genome assembly the diploid relative Aegilops sharonensis use positional mapping, mutagenesis, RNA-Seq transgenesis to identify stem rust gene Sr62 , which has also transferred common wheat. This encodes tandem kinase,...
Abstract Plant nucleotide-binding leucine-rich repeat (NLRs) immune receptors directly or indirectly recognize pathogen-secreted effector molecules to initiate plant defense. Recognition of multiple pathogens by a single NLR is rare and usually occurs via monitoring for changes host proteins; few characterized NLRs have been shown effectors. The barley (Hordeum vulgare) gene Mildew locus (Mla) has undergone functional diversification, the proteins encoded different Mla alleles host-adapted...
Abstract Crop losses caused by plant pathogens are a primary threat to stable food production. Stripe rust ( Puccinia striiformis ) is fungal pathogen of cereal crops that causes significant, persistent yield loss. exhibits host species specificity, with lineages have adapted infect wheat and barley. While stripe barley commonly restricted their corresponding hosts, the genes underlying this specificity remain unknown. Here, we show three resistance genes, Rps6 , Rps7 Rps8 contribute...
Summary Post‐transcriptional gene silencing (PTGS) is a sequence‐specific RNA degradation process conserved in fungi, plants and animals. The trigger of the mechanism double‐stranded derived from transgenic or endogenous loci formed by intra‐ inter‐molecular interactions single‐stranded RNAs action RNA‐dependent polymerases (RDRs). Double‐stranded various sources processed one four Dicer‐like (DCL) proteins Arabidopsis, resulting short enter into at least different pathways, which involves...
In the evolution of land plants, plant immune system has experienced expansion in receptor and signaling pathways. Lineage-specific expansions have been observed diverse gene families that are potentially involved immunity but lack causal association. Here, we show Rps8-mediated resistance barley to pathogen Puccinia striiformis f. sp. tritici (wheat stripe rust) is conferred by a genetic module: Pur1 Exo70FX12, which together necessary sufficient. encodes leucine-rich repeat kinase ortholog...
We identified two novel wheat stem rust resistance genes, Sr-1644-1Sh and Sr-1644-5Sh in Aegilops sharonensis that are effective against widely virulent African races of the pathogen. Stem is one most important diseases world. When single (Sr) genes deployed wheat, they often rapidly overcome by To this end, we initiated a search for sources diverse relatives wild goatgrass species sharonesis (Sharon goatgrass) as rich reservoir to rust. The objectives study were discover map Sr Ae. explore...
The domestication of plants is underscored by the selection agriculturally favorable developmental traits, including flowering time, which resulted in creation varieties with altered growth habits. Research into pathways underlying these habits cereals has highlighted role three main regulators: VERNALIZATION1 (VRN1), VRN2, and FLOWERING LOCUS T (FT). Previous reverse genetic studies suggested that roles VRN1 FT are conserved Brachypodium distachyon yet identified considerable ambiguity...
Nonhost resistance is often conceptualized as a qualitative separation from host resistance. Classification into these two states generally facile, they fail to fully describe the range of that exist in transition nonhost. This poses problem when studying pathosystems cannot be classified either or nonhost due their intermediate status relative extremes. In this study, we investigate efficacy Poaceae-stripe rust (Puccinia striiformis Westend.) interaction for describing host-nonhost...
A method to obtain low molecular weight hydrolysates with a high Fischer ratio (molar of Val + Leu Ile Tyr Phe) is described. Sunflower protein concentrate, concentration in polyphenols, was hydrolyzed kerase pH-stat 16.5% degree hydrolysis. The hydrolysate fractionated by ultrafiltration using membranes cutoffs 30 000 and 5000. obtained fractions, UC-30, UC-5, UF-5, were characterized for distribution amino acid composition. From quantitative point view, the two main fractions UC-5 UF-5....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCharacterization of enzymic sunflower protein hydrolyzatesJ. Parrado, F. Millan, I. Hernandez-Pinzon, J. Bautista, and A. MachadoCite this: Agric. Food Chem. 1993, 41, 11, 1821–1825Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://pubs.acs.org/doi/10.1021/jf00035a003https://doi.org/10.1021/jf00035a003research-articleACS PublicationsRequest reuse permissionsArticle...
Abstract Protecting crops from pests and diseases is vital for the sustainable agricultural systems needed food security. Introducing functional resistance genes to enhance plant immune system an effective method of disease control, but identifying new receptors time-consuming resource intensive. We observed that NLR class show a signature high expression in uninfected plants across both monocot dicot species. Here we this signature, combined with throughput crop transformation, can be used...
Abstract Key message Understanding the molecular network, including protein-protein interactions, of VRS5 provide new routes towards identification other key regulators plant architecture in barley. The TCP transcriptional regulator TEOSINTE BRANCHED 1 (TB1) is a architecture. In barley, an important cereal crop, HvTB1 (also referred to as VULGARE SIX-ROWED spike (VRS) 5), inhibits outgrowth side shoots, or tillers, and grains. Despite its role barley development, there limited knowledge on...
Abstract A method is described to obtain hydrolysates with defined characteristics and a high Fischer ratio for patients liver failure, using sunflower proteins (globulin fraction‐II) as starting material. Protein branched chain amino acid (BCAA) concentration of 29.7±1.7% treated in first step immobilized chymotrypsin (raw hydrolysate‐1). Subsequent ultrafiltration (cut‐off 3 kDa) the hydrolysate gives protein hydrolysate‐I (SFPH‐I). In second step, SFPH‐I carboxypeptidase‐A at alkaline pH...
Plant immune receptors are under constant selective pressure to maintain resistance plant pathogens.Nucleotide-binding leucine-rich repeat (NLR) proteins one class of cytoplasmic whose genes commonly show signatures adaptive evolution 1,2 .While it is known that balancing selection contributes maintaining high intraspecific allelic diversity, the evolutionary mechanism influences transmission alleles during speciation remains unclear.The barley Mla locus has over 30 described conferring...
Abstract Plant nucleotide-binding leucine-rich repeat immune receptors (NLRs) directly or indirectly recognize pathogen-secreted effector molecules to initiate plant defense. Recognition of multiple pathogens by a single NLR is rare and usually occurs via monitoring for changes host proteins; few characterized NLRs have been shown effectors. The barley Mla has undergone functional diversification alleles host-adapted isolates powdery mildew ( Blumeria graminis f. sp. hordei; Bgh ). Here, we...
Retrotransposons' high capacity for mutagenesis is a threat that genomes need to control tightly. Transcriptional gene silencing general and highly effective of retrotransposon expression. Yet, some retrotransposons manage transpose proliferate in plant genomes, suggesting that, as shown viruses, can escape silencing. However no evidence has been reported. Here we analyze the tobacco Tnt1 report even though constructs driven by promoter become silenced when stably integrated tobacco,...
The origin of genetic diversity in a species is product novel mutation and alleles maintained during speciation. Identification that are derived from common ancestor critical for understanding the selective processes experienced speciation events. Here, we report on inter- intra-specific plant immune receptors Mla locus across Poaceae species. These belong to class cytoplasmic-nuclear localized nucleotide-binding leucine-rich repeat (NLR) proteins. Plant under constant pressure maintain...
Aegilops sharonensis Eig (Sharon goatgrass) is a wild diploid relative of wheat within the Sitopsis section Aegilops. This species represents an untapped reservoir genetic diversity for traits agronomic importance, especially as source novel disease resistance. To gain foothold in this resource, we sequenced cDNA from leaf tissue two geographically distinct Ae. accessions (1644 and 2232) using 454 Life Sciences platform. We compared results different assembly programs parameter sets to...