A5052712917- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Enzyme Production and Characterization
- Phytase and its Applications
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Pathogenic Bacteria Studies
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Sphingolipid Metabolism and Signaling
- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Toxin Mechanisms and Immunotoxins
- Lipid Membrane Structure and Behavior
- Autophagy in Disease and Therapy
- Biofuel production and bioconversion
- Seed Germination and Physiology
- Plant responses to water stress
- Plant Genetic and Mutation Studies
- Lipid metabolism and biosynthesis
University of Copenhagen
2009-2018
Sainsbury Laboratory
2012
King Saud University
2011
Heriot-Watt University
2008
University of Edinburgh
2008
Carlsberg Laboratory
1986-1995
Rockefeller University
1988-1995
Max Planck Society
1993
University of St Andrews
1992
University of Hull
1982
We have purified three proteins from barley (Hordeum vulgare L.) seeds which synergistically inhibit the growth of fungi measured in a microtiter well assay. The are 26-kDa chitinase, 30-kDa ribosome-inactivating protein, and 32-kDa (1-3)-beta-glucanase. Full-length cDNAs encoding them were isolated sequenced to determine complete primary structures proteins. Northern hybridizations with as probes showed that corresponding mRNAs accumulate differentially during seed development germination....
cDNAs encoding three proteins from barley (Hordeum vulgare), a class-II chitinase (CHI), beta-1,3-glucanase (GLU) and Type-I ribosome-inactivating protein (RIP) were expressed in tobacco plants under the control of CaMV 35S-promoter. High-level expression transferred genes was detected transgenic by Northern Western blot analysis. The leader peptides CHI GLU led to accumulation these intercellular space leaves. RIP, which is naturally deposited cytosol endosperm cells, either its original...
Abstract Biotic and abiotic stresses limit agricultural yields, plants are often simultaneously exposed to multiple stresses. Combinations of such as heat drought or cold high light intensity have profound effects on crop performance yields. Thus, delineation the regulatory networks metabolic pathways responding single concurrent is required for breeding engineering stress tolerance. Many studies described transcriptome changes in response However, exposure a combination factors may require...
We have previously shown that the expression of a rice gene, rab-16A, is responsive to abscisic acid (ABA) and osmotic stress in plant tissues cultured suspension cells. demonstrate here transcriptional elements between -294 -52 this gene are sufficient confer ABA-dependent on chloramphenicol acetyltransferase reporter protoplasts. Sequence motifs within 242-base-pair region rab-16A conserved among 5' upstream regions other ABA-responsive genes. Gel retardation DNAse I experiments show...
We describe the lethal, recessive accelerated-cell-death11 Arabidopsis mutant (acd11). Cell death in acd11 exhibits characteristics of animal apoptosis monitored by flow cytometry, and constitutively expresses defense-related genes that accompany hypersensitive response normally triggered avirulent pathogens. Global transcriptional changes during programmed cell (PCD) defense activation were cDNA microarray hybridization. The PCD pathways activated are salicylic acid (SA) dependent, but do...
Summary Arabidopsis MPK4 has been implicated in plant defense regulation because mpk4 knockout plants exhibit constitutive activation of salicylic acid (SA)‐dependent defenses, but fail to induce jasmonic (JA) marker genes response JA. We show here that mutants are also defective gene induction ethylene (ET), and they more susceptible than wild‐type (WT) Alternaria brassicicola induces the ET/JA pathway(s). Both SA‐repressing ET/JA‐(co)activating functions depend on kinase activity involve...
Abstract The Arabidopsis (Arabidopsis thaliana) MKK1 and MKK2 mitogen-activated protein kinase kinases have been implicated in biotic abiotic stress responses as part of a signaling cascade including MEKK1 MPK4. Here, the double loss-of-function mutant (mkk1/2) is shown to marked phenotypes development disease resistance similar those single mekk1 mpk4 mutants. Because mkk1 or mkk2 mutants appear wild type, basal levels MPK4 activity are not impaired them, functionally redundant unchallenged...
Light is a major environmental cue affecting various physiological and metabolic processes in plants. Although plant photoreceptors are well characterized, the mechanisms by which light regulates downstream responses less clear. In Arabidopsis thaliana, accumulation of photoprotective anthocyanin pigments dependent, R2R3 MYB transcription factor MYB75/PAP1 accumulation. Here, we report that MYB75 interacts with phosphorylated MAP KINASE4 (MPK4). Their interaction dependent on MPK4 kinase...
Oil bodies are lipid storage organelles which have been analyzed biochemically due to the economic importance of oil seeds. Although structurally simple, mechanisms involved in their formation and degradation remain controversial. At present, only two proteins associated with described, oleosin caleosin. Oleosin is thought be important for body stabilization cytosol, although neither structure nor function has fully elucidated. Even less known about caleosin, recently described [Chen et al....
We have used a transient expression assay in aleurone protoplasts of barley to delineate hormone response elements the abscisic acid (ABA)-responsive rice gene Rab16A and gibberellin A3 (GA3)-responsive alpha-amylase Amy 1/6-4. Our approach transcriptional fusions between their 5' upstream sequences bacterial chloramphenicol acetyltransferase reporter gene. A chimeric promoter containing six copies -181 -171 region Rab 16A fused minimal conferred ABA-responsive on Transcription from this ABA...
Summary A genomic DNA fragment was isolated containing 5′ upstream sequences and part of the open reading frame corresponding to lipoxygenase 1 cDNA ( LoxA ) expressed in barley grains during development germination. Lox1 transcription shown be methyl jasmonate (MeJA)‐ wound‐inducible leaves, but transcripts were not detected mildew‐infected although this is a commonly observed response pathogenic attack various plants. Transient gene expression assays used identify promoter region involved...
Summary GAMYB is an MYB transcription factor which expressed in cereal aleurone cells response to gibberellin (GA). HvGAMYB binds the TAACAAA box of a high‐pI α‐amylase gene promoter and transcriptionally activates its expression. In this study, we examined role activating expression other GA‐regulated genes encoding hydrolytic enzymes. transient experiments, transactivated reporter fused low‐pI promoter, EII(1–3,1–4)‐β‐glucanase cathepsin B‐like protease promoter. DNA binding specificity...