A5064166269- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Genetics and Plant Breeding
- Chromosomal and Genetic Variations
- Yeasts and Rust Fungi Studies
- Plant pathogens and resistance mechanisms
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- Plant Pathogens and Resistance
- Fungal Biology and Applications
- Plant Reproductive Biology
- Plant Genetic and Mutation Studies
- Fungal and yeast genetics research
- Cell death mechanisms and regulation
- Turfgrass Adaptation and Management
- Agricultural Productivity and Crop Improvement
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Botanical Research and Applications
Tel Aviv University
2014-2024
Abstract The wild relatives and progenitors of wheat have been widely used as sources disease resistance ( R ) genes. Molecular identification characterization these genes facilitates their manipulation tracking in breeding programmes. Here, we develop a reference-quality genome assembly the diploid relative Aegilops sharonensis use positional mapping, mutagenesis, RNA-Seq transgenesis to identify stem rust gene Sr62 , which has also transferred common wheat. This encodes tandem kinase,...
SUMMARY Aegilops is a close relative of wheat ( Triticum spp.), and species in the section Sitopsis represent rich reservoir genetic diversity for improvement wheat. To understand their advance utilization, we produced whole‐genome assemblies longissima speltoides . Whole‐genome comparative analysis, along with recently sequenced sharonensis genome, showed that Ae. genomes are highly similar most closely related to D subgenome. By contrast, genome more B Haplotype block analysis supported...
In search of Botrytis cinerea cell death-inducing proteins, we found a xyloglucanase (BcXYG1) that induced strong necrosis and resistance response in dicot plants. Expression the BcXYG1 gene was strongly during first 12 h post inoculation, analysis disease dynamics using PathTrack showed B. strain overexpressing produced early local necrosis, supporting role as an factor. The activity not necessary for induction plant resistance, mutant lacking enzymatic retained both functions. Residues two...
Nucleotide-binding leucine-rich repeat (NLR) disease resistance genes typically confer against races of a single pathogen. Here, we report that Yr87/Lr85, an NLR gene from Aegilops sharonensis and longissima, confers both P. striiformis tritici (Pst) Puccinia triticina (Pt) cause stripe leaf rust, respectively. Yr87/Lr85 Pst Pt in wheat introgression as well transgenic lines. Comparative analysis the cloned Triticeae shows contains two distinct LRR domains is only found Ae. longissima....
Abstract Background Leaf and stripe rusts are two major wheat diseases, causing significant yield losses. The preferred way for protecting from rust pathogens is by introgression of resistance traits wheat-related wild species. To avoid genetic drag due to replacement large chromosomal segments the alien chromatin, it necessary shorten chromosome segment in primary recombinants. Results Here we report on shortening an that carries leaf Sharon goatgrass (Aegilops sharonensis) . Rust resistant...
Abstract The wild relatives and progenitors of wheat have been widely used as sources disease resistance ( R ) genes. Molecular identification characterization these genes facilitates their manipulation tracking in breeding programmes. We developed a reference-quality genome assembly the diploid relative Aegilops sharonensis positional mapping, mutagenesis, RNA-Seq transgenesis to identify stem rust gene Sr62 , which was also transferred common wheat. This encodes tandem kinase, homologues...
Abstract Nucleotide-binding site leucine-rich repeat (NLR) disease-resistance genes typically confer resistance against races of a single pathogen. We report that Lr/Yr548, an NLR gene from Aegilops sharonensis and longissima, confers specific Puccinia triticina (Pt) P. striiformis tritici (Pst) cause leaf stripe rust, respectively. Lr/Yr548 prevented disease development in wheat introgression as well transgenic lines. Comparative analysis all cloned Triticeae showed contains distinctive...
Rac proteins are involved in a variety of cellular processes. Effector that interact with active convey the GTPase-generated signal to downstream developmental cascades and Here we report on analysis main effector cascade BcRac, homolog grey mold fungus Botrytis cinerea. Several lines evidence highlighted p21-activated kinase Cla4 as an important fungi. Analysis Δbccla4 strains revealed BcCla4 protein was sufficient mediate all examined BcRac-driven processes, including hyphal growth...
Measurement of apoptosis by SCAN©, a system for counting and analysis fluorescently labelled nuclei – INTRODUCTION Apoptosis was initially described in animals, where it plays central roles development homeostasis . Activation apoptosis, external or internal stimuli, drives set coordinated intracellular processes, which terminate with the formation apoptotic bodies cell death. The most significant processes that define include ions protein leakage from (...)
Abstract Aegilops is a close relative of wheat ( Triticum spp.), and species in the section Sitopsis represent rich reservoir genetic diversity for improvement wheat. To understand their advance utilization, we produced whole-genome assemblies Ae. longissima speltoides . Whole-genome comparative analysis, along with recently sequenced sharonensis genome, showed that genomes are highly similar most closely related to D subgenome. By contrast, genome more B Haplotype block analysis supported...
Genetic engineering of wheat is complex due to its large genome size, the presence numerous genes with high sequence similarities, and a multitude repetitive elements. In addition, genetic transformation has been difficult, mainly poor regeneration in tissue cultures. Recent advances plant biotechnology, particularly use regenerative GROWTH-REGULATING FACTOR (GRF) GRF-INTERACTING (GIF), have provided new tools for regeneration. Another transformative tool RUBY system, that involves three...