A5022706316- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Neurobiology and Insect Physiology Research
- Plant Virus Research Studies
- Genetic diversity and population structure
- Insect Resistance and Genetics
- Genetics, Aging, and Longevity in Model Organisms
- Physiological and biochemical adaptations
- Genetic and phenotypic traits in livestock
- Plant Pathogens and Fungal Diseases
- Bioinformatics and Genomic Networks
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Animal Behavior and Reproduction
- Studies on Chitinases and Chitosanases
- Migraine and Headache Studies
- Evolutionary Algorithms and Applications
- Genetics and Physical Performance
- Enzyme Production and Characterization
- Plant Pathogens and Resistance
- Neuroscience of respiration and sleep
- Soybean genetics and cultivation
Center for Grain and Animal Health Research
2019-2025
Kansas State University
2015-2024
Agricultural Research Service
2021-2024
United States Department of Agriculture
2021-2024
North Carolina State University
2006-2012
W. M. Keck Foundation
2009
RMIT University
2000
A major challenge of biology is understanding the relationship between molecular genetic variation and in quantitative traits, including fitness. This determines our ability to predict phenotypes from genotypes understand how evolutionary forces shape within species. Previous efforts dissect genotype–phenotype map were based on incomplete genotypic information. Here, we describe Drosophila melanogaster Genetic Reference Panel (DGRP), a community resource for analysis population genomics...
Wheat (Triticum spp.) is one of the founder crops that likely drove Neolithic transition to sedentary agrarian societies in Fertile Crescent more than 10,000 years ago. Identifying genetic modifications underlying wheat's domestication requires knowledge about genome its allo-tetraploid progenitor, wild emmer (T. turgidum ssp. dicoccoides). We report a 10.1-gigabase assembly 14 chromosomes tetraploid wheat, as well analyses gene content, architecture, and diversity. With this fully assembled...
Epistasis—nonlinear genetic interactions between polymorphic loci—is the basis of canalization and speciation, epistatic can be used to infer networks affecting quantitative traits. However, role that epistasis plays in architecture traits is controversial. Here, we compared three Drosophila life history sequenced inbred lines melanogaster Genetic Reference Panel (DGRP) a large outbred, advanced intercross population derived from 40 DGRP (Flyland). We assessed allele frequency changes pools...
Bread wheat is an allopolyploid species with a large, highly repetitive genome. To investigate the impact of selection on variants distributed among homoeologous genomes and to build foundation for understanding genotype-phenotype relationships, we performed population-scale re-sequencing diverse panel lines.A sample 62 lines was re-sequenced using whole exome capture genotyping-by-sequencing approaches. We describe allele frequency, functional significance, chromosomal distribution 1.57...
Wheat blast is a serious disease caused by the fungus Magnaporthe oryzae ( Triticum pathotype) (MoT). The objective of this study was to determine effect 2NS translocation from Aegilops ventricosa (Zhuk.) Chennav on wheat head and leaf resistance. Disease phenotyping experiments were conducted in growth chamber, greenhouse, field environments. Among 418 cultivars aestivum L.), those with had 50.4 72.3% less than without when inoculated an older MoT isolate under chamber conditions. When...
Summary Recombination affects the fate of alleles in populations by imposing constraints on reshuffling genetic information. Understanding basis these is critical for manipulating recombination process to improve resolution mapping, and reducing negative effects linkage drag deleterious load breeding. Using sequence‐based genotyping a wheat nested association mapping ( NAM ) population 2,100 recombinant inbred lines created crossing 29 diverse lines, we mapped QTL affecting distribution...
Abstract Allopolyploidy greatly expands the range of possible regulatory interactions among functionally redundant homoeologous genes. However, connection between emerging complexity and expression phenotypic diversity in polyploid crops remains elusive. Here, we use diverse wheat accessions to map quantitative trait loci (eQTL) evaluate their effects on population-scale variation homoeolog dosage. The relative contribution cis - trans -eQTL is strongly affected by both selection demographic...
Reactive oxygen species (ROS) are a common byproduct of mitochondrial energy metabolism, and can also be induced by exogenous sources, including UV light, radiation, environmental toxins. ROS generation is essential for maintaining homeostasis triggering cellular signaling pathways host defense mechanisms. However, an imbalance induces oxidative stress death associated with human disease, age-related locomotor impairment. To identify genes affecting sensitivity resistance to ROS-induced...
Abstract Background Obesity and phenotypic traits associated with this condition exhibit significant heritability in natural populations of most organisms. While a number genes genetic pathways have been implicated to play role obesity traits, the architecture that underlies variation these is largely unknown. Here, we used 40 wild-derived inbred lines Drosophila melanogaster quantify body weight, content three major metabolites (glycogen, triacylglycerol, glycerol) obesity, metabolic rate...
Abstract Background Locomotion is an integral component of most animal behaviors, and many human health problems are associated with locomotor deficits. Locomotor behavior a complex trait, population variation attributable to interacting loci small effects that sensitive environmental conditions. However, the genetic basis this largely uncharacterized. Results We quantified Drosophila melanogaster in large inbred lines derived from single natural population, replicated selection different...
Awns are stiff, hair-like structures which grow from the lemmas of wheat (Triticum aestivum) and other grasses that contribute to photosynthesis play a role in seed dispersal. Variation awn length domesticated is controlled primarily by three major genes, most commonly dominant suppressor Tipped1 (B1). This study identifies transcription repressor responsible for inhibition at B1 locus. Association mapping was combined with analysis biparental populations delimit distal region 5AL...
Abstract Background Our understanding of how the complexity wheat genome influences distribution chromatin states along homoeologous chromosomes is limited. Using a differential nuclease sensitivity assay, we investigate coding and repetitive regions allopolyploid genome. Results Although open found to be significantly enriched around genes, majority MNase-sensitive are located within transposable elements (TEs). Chromatin smaller D more accessible than that larger A B genomes. different TEs...
Locomotion is an integral component of most animal behaviors and many human diseases disorders are associated with locomotor deficits, but little known about the genetic basis natural variation in behavior. a complex trait, attributable to joint segregation multiple interacting quantitative trait loci (QTL), effects that sensitive environment. We assessed behavior (locomotor reactivity) population 98 recombinant inbred lines Drosophila melanogaster mapped four QTL affecting reactivity by...
Abstract Puccinia graminis f.sp. tritici (Pgt) causes stem rust disease in wheat that can result severe yield losses. The factors driving the evolution of its virulence and adaptation remain poorly characterized. We utilize long-read sequencing to develop a haplotype-resolved genome assembly U.S. isolate Pgt. Using Pgt haplotypes as reference, we characterize structural variants (SVs) single nucleotide polymorphisms diverse panel isolates. SVs impact repertoire predicted effectors, secreted...
Abstract Barley yellow dwarf (BYD) is one of the most serious viral diseases in cereal crops worldwide. Identification quantitative trait loci (QTLs) underlining wheat resistance to barley virus (BYDV) essential for breeding BYDV‐tolerant cultivars. In this study, a recombinant inbred line (RIL) population was developed from cross between Jagger (PI 593688) and mutant (JagMut1095). A linkage map 3106 cM consisting 21 chromosomes using 1003 unique single nucleotide polymorphisms (SNPs) RIL...
Abstract Viral pathogens adversely affect wheat ( Triticum aestivum L.) development and are responsible for significant yield losses. Barley yellow dwarf virus (BYDV) is one of the most serious worldwide threats to cereal crops. Soil‐borne mosaic (SBWMV) has been present in Great Plains damage over a century. Identification additional sources genetic resistance paramount combat potential from these viruses. We constructed panel 269 winter cultivars breeding lines assess naturally occurring...
Identification of risk alleles for human behavioral disorders through genomewide association studies (GWAS) has been hampered by a daunting multiple testing problem. This problem can be circumvented some phenotypes combining in model organisms with subsequent candidate gene analyses populations. Here, we characterized genetic networks that underlie the response to ethanol exposure Drosophila melanogaster measuring knockdown time 40 wild-derived inbred lines. We associated phenotypic...
Genome-wide single nucleotide polymorphism (SNP) variation allows for the capture of haplotype structure in populations and prediction unobserved genotypes based on inferred regions identity-by-descent (IBD). Here we have used a first-generation wheat map created by targeted re-sequencing low-copy genomic reference panel 62 lines to impute marker diverse winter cultivars from U.S. Great Plains. The IBD segments between population were identified SNP genotyped using 90K iSelect array...
Whole-genome shotgun resequencing of wheat is expensive because its large, repetitive genome. Moreover, sequence data can fail to map uniquely the reference genome, making it difficult unambiguously assign variation. Resequencing using target capture enables sequencing large numbers individuals at high coverage reliably identify variants associated with important agronomic traits. Previous studies have implemented complementary DNA/exon or gene-based probe sets in which promoter and intron...
Stripe rust is a devastating disease in wheat that causes substantial yield loss around the world. The most effective strategy for mitigating to develop resistant cultivars. wild relatives of are good sources resistance fungal pathogens. Here, we used genome-wide association study (GWAS) identify loci associated with stripe (causal agent: Puccinia striiformis f. sp. tritici) emmer (Triticum dicoccoides) at seedling stage, greenhouse, and adult plant field. We found two major contributing our...
Abstract Introgression from wild relatives is a valuable source of novel allelic diversity for breeding. We investigated the genomic patterns introgression Aegilops tauschii, diploid ancestor wheat D genome, into winter (Triticum aestivum) cultivars. The population 351 BC1F3:5 lines was selected based on phenology crosses between six hexaploid and 21 wheat-Ae. tauschii octoploids. SNP markers developed this diverse panel 116 Ae. accessions by complexity-reduced genome sequencing were used to...
Glycoside hydrolases (GHs, also called glycosidases) catalyze the hydrolysis of glycosidic bonds in polysaccharides. Numerous GH genes have been identified from various organisms and are classified into 188 families, abbreviated GH1 to GH188. Enzymes GH32 family hydrolyze fructans, which present approximately 15% flowering plants widespread across microorganisms. rarely found animals, as fructans not a typical carbohydrate source utilized animals. Here, we report discovery 242 84 animal...