A5017026987- Probiotics and Fermented Foods
- Botulinum Toxin and Related Neurological Disorders
- Biochemical and Structural Characterization
- Genomics and Phylogenetic Studies
- Streptococcal Infections and Treatments
- Bacteriophages and microbial interactions
- Microbial Metabolites in Food Biotechnology
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Hereditary Neurological Disorders
- Neurological disorders and treatments
- Listeria monocytogenes in Food Safety
- Enzyme Production and Characterization
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolism and Applications
- Glycosylation and Glycoproteins Research
- Essential Oils and Antimicrobial Activity
- Milk Quality and Mastitis in Dairy Cows
- Galectins and Cancer Biology
- Antimicrobial Resistance in Staphylococcus
- Antimicrobial Peptides and Activities
- Respiratory and Cough-Related Research
- Pathogenesis and Treatment of Hiccups
University of Helsinki
2016-2025
University College Cork
2008-2016
Abstract Lactobacilli are a diverse group of species that occupy nutrient-rich niches associated with humans, animals, plants and food. They used widely in biotechnology food preservation, being explored as therapeutics. Exploiting lactobacilli has been complicated by metabolic diversity, unclear identity uncertain relationships between them other commercially important lactic acid bacteria. The capacity for biotransformations catalysed is an untapped resource. Here we report the genome...
Lactobacillus rhamnosus is a lactic acid bacterium that found in large variety of ecological habitats, including artisanal and industrial dairy products, the oral cavity, intestinal tract or vagina. To gain insights into genetic complexity versatility species L. rhamnosus, we examined genomes phenotypes 100 strains isolated from diverse sources. The were mapped onto GG reference genome. These phenotypically characterized for wide range metabolic, antagonistic, signalling functional...
Intestinimonas is a newly described bacterial genus with representative strains present in the intestinal tract of human and other animals. Despite unique metabolic features including production butyrate from both sugars amino acids, there to date no data on their diversity, ecology, physiology. Using comprehensive phylogenetic approach, Intestinimomas was found include at least three species that colonize primarily mouse intestine. We focused most common cultivable genus, butyriciproducens,...
Vancomycin-resistant enterococci (VRE) have become a major nosocomial threat. Enterococcus faecium is of special concern, as it can easily acquire new antibiotic resistances and an excellent colonizer the human intestinal tract. Several clinical studies explored potential use beneficial bacteria to weed out opportunistic pathogens. Specifically, widely studied Lactobacillus rhamnosus strain GG has been applied successfully in context VRE infections. Here, we provide insight into molecular...
Bifidobacteria are natural inhabitants of the human gastrointestinal tract and well known for their health-promoting effects. Tolerance to bile stress is crucial bifidobacteria survive in colon exert beneficial actions. In this work, RNA-Seq transcriptomic analysis complemented with proteomic was used investigate cellular response Bifidobacterium longum BBMN68. The transcript levels 236 genes were significantly changed (≥ threefold, p < 0.001) 44 proteins differentially abundant (≥1.6-fold,...
The lactic acid bacterium Lactobacillus rhamnosus GG is the most studied probiotic with proven health benefits upon oral intake, including alleviation of diarrhea. mission Yoba for Life foundation to provide impoverished communities in Africa increased access under name yoba 2012, world's first generic strain. We have been able overcome strain's limitations grow food matrices like milk, by formulating a dried starter consortium Streptococcus thermophilus that enables propagation both strains...
Lactobacillus rhamnosus is a diverse Gram-positive species with strains isolated from different ecological niches. Here, we report the genome sequence analysis of 40 L. and their genomic comparison, focus on variable genome. Genomic comparison discriminated conserved genes (core genome) regions plasticity involving frequent rearrangements horizontal transfer (variome). The core encompasses 2,164 genes, out 4,711 in total (the pan-genome). accessory dominated by encoding carbohydrate...
Human infection by the gastric pathogen Helicobacter pylori is characterized a robust immune response which rarely prevents persistent H. colonization. Emerging evidence suggests that lactobacilli may reduce rates and associated inflammation. In this study, we measured ability of two model strains Lactobacillus salivarius (UCC118 UCC119) to modulate epithelial cell chemokine responses infection. Pre-treatment AGS cells with either L. strain significantly decreased interleukin-8 (IL-8)...
To overcome the deleterious effects of acid stress, Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus) elicits an adaptive response to stress. In this study, proteomics approach complemented by transcriptional analysis revealed some cellular changes in L. CAUH1 during adaptation. We observed increase glycolysis-associated proteins, promoting optimal utilization carbohydrates. Also, rerouting pyruvate metabolism fatty biosynthesis was observed, indicating a possible modification cell...
ABSTRACT The draft genomes of Lactobacillus plantarum strains isolated from Asian fermented foods, infant feces, and shrimp intestines were sequenced compared to those well-studied strains. Among 28 L. , variations in the genomic features involved ecological adaptation elucidated. genome sizes ranged approximately 3.1 3.5 Mb, which about 2,932 3,345 protein-coding sequences (CDS) predicted. food-derived isolates contained a higher number carbohydrate metabolism-associated genes than feces....
Mapping of the microbial molecules underlying microbiota-host interactions is key to understand how microbiota preserve mucosal homeostasis. A pivotal family such bacterial are pili. Pili proteinaceous cell wall appendages with a well-documented role in adhesion, whilst their immune interaction host less established. Gram-positive pili often posttranslationally modified by sortase-specific cleavage reactions and formation intramolecular peptide bonds. Here we report glycosylation as new...
ABSTRACT Insects play a significant role in the transmission and spread of bacterial pathogens that cause various diseases humans animals. The relationship among insects, is complex depends on specificity pathogens. Some clostridial species produce botulinum neurotoxin (BoNT), which responsible for paralytic botulism. However, ecology these their non‐toxinogenic phylogenetic counterparts remains unclear. This study specifically explored silico evidence interconnection between BoNT‐producing...
ABSTRACT Many phages employ a large heteropolymeric organelle located at the tip of tail, termed baseplate, for host recognition. Contrast electron microscopy (EM) lactococcal phage Tuc2009 baseplate and its host-binding subunits, so-called tripods, allowed us to obtain low-resolution structural image this organelle. Structural comparisons between related TP901-1 that demonstrated they are highly similar, except presence an additional protein in (BppA ), which is attached top tripod...
Several probiotic-marketed formulations available for the consumers contain live lactic acid bacteria and/or bifidobacteria. The multispecies product commercialized as VSL#3 has been used treating various gastro-intestinal disorders. However, like many other products, bacterial strains present in have only characterized to a limited extent and their efficacy well predicted mode of action remain unclear, preventing further applications or comparative studies. In this work, genomes all eight...
Clostridium botulinum poses a serious threat to food safety and public health by producing potent neurotoxin during its vegetative growth causing life-threatening neuroparalysis, botulism. While high temperature can be utilized eliminate C. spores the neurotoxin, non-thermal elimination of newly germinated cells before onset toxin production could provide an alternative or additional factor controlling risk botulism in some applications. Here we introduce putative phage lysin that...
Listeria monocytogenes is a foodborne pathogen and resilient environmental saprophyte. Dairy farms are reservoir of L. monocytogenes, strains can persist on for years. Here, we sequenced the genomes 250 isolates to investigate persistence mobile genetic elements (MGEs)
Lactobacillus rhamnosus GG is a lactic acid bacterium widely marketed by the food industry. Its genomic analysis led to identification of gene cluster encoding mucus-binding SpaCBA pili, which located in island enriched insertion sequence (IS) elements. In present study, we analyzed genome-wide resequencing integrity L. four distinct evolutionary experiments conducted for approximately 1,000 generations under conditions no stress or salt, bile, and repetitive-shearing stress. Under both...
Clostridium botulinum produces the neurotoxin that causes botulism, a rare but potentially lethal paralysis. Endospores play an important role in survival, transmission, and pathogenesis of C. botulinum. strains are very diverse, both genetically ecologically. Group I terrestrial, mesophilic, produce highly heat-resistant spores, while II can be terrestrial (type B) or aquatic E) generally psychrotrophic spores moderate heat resistance. III either aquatic, mesophilic slightly thermophilic,...
Summary The members of the L actobacillus genus are widely used in food and feed industry show a remarkable ecological adaptability. Several strains have been marketed as probiotics they possess health‐promoting properties for host. In present study, we two complementary next‐generation sequencing technologies to deduce genome sequences casei LcA LcY , which were isolated from products A ctimel Y akult, commercialized probiotics. draft genomes have, respectively, an estimated size 3067 3082...
Abstract Background The development of the Nisin Inducible Controlled Expression (NICE) system in food-grade bacterium Lactococcus lactis subsp. cremoris represents a cornerstone use Gram-positive bacterial expression systems for biotechnological purposes. However, proteins that are subjected to such over-expression L. may suffer from improper folding, inclusion body formation and/or protein degradation, thereby significantly reducing yield soluble target protein. Although drawbacks not...
Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and the human body where they commensals naturally present normal microbiota composition of gut, vagina skin. However, some cases, spp. have been implicated bacteremia. The aim study was to examine genomic immunological properties 16 clinical blood isolates L. compare them well-studied probiotic strain GG. Blood cultures from bacteremic patients were collected at Helsinki University Hospital laboratory 2005-2011 isolated...
Abstract Background Intestinal botulism is primarily reported in small babies as a condition known infant botulism. The results from the ingestion of environmental or foodborne spores botulinum neurotoxin (BoNT) producing Clostridia , usually Clostridium and subsequent spore germination into active neurotoxinogenic cultures gut. It generally considered that are susceptible to C. colonization because their immature gut microbiota. Yet, it poorly understood which host factors contribute...