A5044970489- Probiotics and Fermented Foods
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Genomics and Phylogenetic Studies
- Meat and Animal Product Quality
- Cystic Fibrosis Research Advances
- Viral gastroenteritis research and epidemiology
- Milk Quality and Mastitis in Dairy Cows
- Genetically Modified Organisms Research
- Microscopic Colitis
- Food composition and properties
- Microbial Metabolites in Food Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Animal Genetics and Reproduction
- Insect Resistance and Genetics
- Antimicrobial Peptides and Activities
- Inflammatory Bowel Disease
- Helicobacter pylori-related gastroenterology studies
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Animal Nutrition and Physiology
- Gastrointestinal motility and disorders
- Listeria monocytogenes in Food Safety
- Polyamine Metabolism and Applications
- CRISPR and Genetic Engineering
Genesys (United States)
2025
Teagasc - The Irish Agriculture and Food Development Authority
2015-2024
Unisys (United States)
2024
Yellowstone Christian College
2024
University College Cork
2014-2023
APC Microbiome Institute
2011-2023
University of South Carolina
2016
National University of Ireland
2009-2015
Alimentary Health (Ireland)
2010-2015
Federico II University Hospital
2015
The last decade has seen numerous outbreaks of Clostridium difficile -associated disease (CDAD), which presented significant challenges for healthcare facilities worldwide. We have identified and purified thuricin CD, a two-component antimicrobial that shows activity against C. in the nanomolar range. Thuricin CD is produced by Bacillus thuringiensis DPC 6431, bacterial strain isolated from human fecal sample, it consists two distinct peptides, Trn-α Trn-β, act synergistically to kill wide...
Abstract Lactobacilli are a diverse group of species that occupy nutrient-rich niches associated with humans, animals, plants and food. They used widely in biotechnology food preservation, being explored as therapeutics. Exploiting lactobacilli has been complicated by metabolic diversity, unclear identity uncertain relationships between them other commercially important lactic acid bacteria. The capacity for biotransformations catalysed is an untapped resource. Here we report the genome...
Lactococcus lactis DPC3147, a strain isolated from an Irish kefir grain, produces bacteriocin with broad spectrum of inhibition. The produced is heat stable, particularly at low pH, and inhibits nisin-producing (Nip+) lactococci. On the basis observation that nisin structural gene (nisA) does not hybridize to DPC3147 genomic DNA, was considered novel designated lacticin 3147. genetic determinants which encode 3147 are contained on 63-kb plasmid, conjugally mobilized commercial cheese...
Vancomycin, metronidazole, and the bacteriocin lacticin 3147 are active against a wide range of bacterial species, including Clostridium difficile . We demonstrate that, in human distal colon model, addition each three antimicrobials resulted significant decrease numbers C. However, their therapeutic use gastrointestinal tract may be compromised by broad spectrum activity, which would expected to significantly impact on other members gut microbiota. used high-throughput pyrosequencing...
Background High-throughput sequencing has enabled detailed insights into complex microbial environments, including the human gut microbiota. The accuracy of data however, is reliant upon appropriate storage samples prior to DNA extraction. aim this study was conduct first MiSeq investigation effects faecal on microbiota, compared fresh samples. Culture-based analysis also completed. Methods Seven were collected from healthy adults. Samples separated (DNA extracted immediately), snap frozen...
species are widely used as probiotics and starter cultures for a variety of foods, supported by long history safe usage. Although more than 35 meet the European Food Safety Authority (EFSA) criteria qualified presumption safety status,
In all, 4379 isolates from 35 products, including 24 artisanal cheeses, were surveyed with a view to identifying strains that could be used as starters in commercial dairy fermentations. Of the isolates, 38% classified Lactococcus , 17% Enterococcus 14% Streptococcus thermophilus 12% mesophilic Lactobacillus 10% Leuconostoc and 9% thermophilic . Acid production by varied considerably. 1582 of 482 tested, only 8 2% respectively produced sufficient acid lower pH milk <5·3 6 h at 30°C....
Clostridium difficile is an important nosocomial pathogen associated particularly with diarrheal disease in elderly individuals hospitals and long-term care facilities. We examined the carriage rate of by culture as a function fecal microbiota composition subjects recruited from community, including outpatient, short-term respite, hospital stay subjects. The ranged 1.6% (n = 123) for to 9.5% 43) outpatient settings, increasing 21% 151) patients short- or hospital. dominant 072 ribotype was...
OBJECTIVES: Comorbidity with Clostridiumdifficilemay cause diagnostic delay in newly presenting inflammatory bowel disease (IBD) patients, trigger relapse established disease, confound therapies, and serve as an indicator of underlying defect innate immunity. Retrospective analyses have suggested community acquisition; to address this we conducted a prospective analysis ofC. difficilecarriage IBD patients using molecular methods specifically outpatient setting. METHODS: Recruited...
Clostridium difficile-associated diarrhoea (CDAD) is the most common hospital-acquired diarrhoea, and a major type of gastroenteritis infection in nursing homes facilities for elderly. In this study antimicrobial activity two-component lantibiotic, lacticin 3,147, against range genetically distinct C. difficile isolates was studied. The bacteriocin exhibited an MIC(50) 3.6 microg ml(-1) 10 strains isolated from healthy subjects, inflammatory bowel disease patients culture collection strains....
Abstract The volatiles responsible for the typical aroma of cheese are produced mainly by lipolytic and proteolytic pathways metabolism lactose, lactate, citrate. volatile profile is determined gas chromatography (GC), which includes extraction, separation, detection volatiles. A wide range extraction techniques available, technological improvements have been developed in GC separation that enhance our understanding role individual key to flavor. To date, surface‐ripened cheese, main...
The demand for novel antimicrobial therapies due to the threat posed by resistance has resulted in a growing interest protective role of our skin bacteria and importance competition among on skin. A survey cultivable human was undertaken identify capacity microbiota produce bacteriocins with activity against pathogens. Twenty-one produced isolated from seven sites body each subject exhibited inhibition spectra ranging broad narrow range, inhibiting many Gram-positive bacteria, including...
Cystic Fibrosis (CF) is an autosomal recessive disease that affects the function of a number organs, principally lungs, but also gastrointestinal tract. The manifestations cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction in tract, as well frequent antibiotic exposure, undoubtedly disrupts gut microbiota. To analyse effects CF and its management on microbiome, we compared microbiota 43 individuals with during period stability, to 69 non-CF controls using...
Abstract Lactobacilli constitute a large genus of Gram-positive lactic acid bacteria which have widespread roles ranging from gut commensals to starters in fermented foods. A combination silico and laboratory-based screening allowed us determine the overall bacteriocin producing potential representative strains each species genus. The genomes 175 lactobacilli 38 associated were screened for presence antimicrobial genes combined with activity against range indicators. There also appears be...
This study describes the characterization of nisin J, first example a natural variant, produced by human skin isolate staphylococcal origin. Nisin J displays inhibitory activity against wide range bacterial targets, including MRSA. work demonstrates potential commensals as source for novel antimicrobials that could form part solution to antibiotic resistance across broad pathogens.
Production of smear-ripened cheese critically depends on the surface growth multispecies microbial consortia comprising bacteria and yeasts. These microorganisms often originate from cheese-making facility and, over many years, have developed into rather stable, dairy-specific associations. While commercial smear starters are frequently used, it is unclear to what degree these able establish successfully within resident consortia. Thus, fate a German Limburger subjected "old-young" smearing...