Effie Tsakalidou

ORCID: 0000-0001-8048-9421
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About
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Research Areas
  • Probiotics and Fermented Foods
  • Gut microbiota and health
  • Microbial Metabolites in Food Biotechnology
  • Protein Hydrolysis and Bioactive Peptides
  • Genomics and Phylogenetic Studies
  • Meat and Animal Product Quality
  • Milk Quality and Mastitis in Dairy Cows
  • Food composition and properties
  • Oral microbiology and periodontitis research
  • Streptococcal Infections and Treatments
  • Microbial Metabolic Engineering and Bioproduction
  • Fermentation and Sensory Analysis
  • Essential Oils and Antimicrobial Activity
  • Identification and Quantification in Food
  • Bacteriophages and microbial interactions
  • Enzyme Catalysis and Immobilization
  • Proteins in Food Systems
  • Animal Nutrition and Physiology
  • Listeria monocytogenes in Food Safety
  • Biofuel production and bioconversion
  • Bacterial Genetics and Biotechnology
  • Microbial Metabolism and Applications
  • Microbial Inactivation Methods
  • Bacterial Identification and Susceptibility Testing
  • Edible Oils Quality and Analysis

Agricultural University of Athens
2016-2025

National Centre of Scientific Research "Demokritos"
2016

Vrije Universiteit Brussel
2004

ABSTRACT We studied the potential inhibitory effect of Lactobacillus casei strain Shirota (from fermented milk product Yakult [Yakult Ltd., Tokyo, Japan]) on Helicobacter pylori by using (i) in vitro inhibition assays with H. SS1 (Sydney 1) and nine clinical isolates (ii) vivo mouse model infection over a period 9 months. In activity against all was observed presence viable L. cells but not cell-free culture supernatant, although there profound urease activity. experiments were performed...

10.1128/aem.70.1.518-526.2004 article EN Applied and Environmental Microbiology 2004-01-01

Lactic acid bacteria (LAB) were isolated from Greek traditional wheat sourdoughs manufactured without the addition of baker's yeast. Application sodium dodecyl sulfate-polyacrylamide gel electrophoresis total cell protein, randomly amplified polymorphic DNA-PCR, DNA-DNA hybridization, and 16S ribosomal DNA sequence analysis, in combination with physiological traits such as fructose fermentation mannitol production, allowed us to classify into species Lactobacillus sanfranciscensis, brevis,...

10.1128/aem.68.12.6059-6069.2002 article EN Applied and Environmental Microbiology 2002-11-26

ABSTRACT The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish ( Sparus aurata ) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis whole-cell proteins performed to identify a total 106 Pseudomonas strains isolated from S. temperatures (at 0, 10, 20°C) packaging (air modified atmosphere 40% CO 2 -30% N O ). lundensis was the predominant species, followed by fluorescens , while fragi...

10.1128/aem.68.1.65-72.2002 article EN Applied and Environmental Microbiology 2002-01-01

Kefir is a high nutritional fermented dairy beverage associated with wide range of health benefits. It constitutes unique symbiotic association, comprising mainly lactic acid bacteria, yeasts, and occasionally acetic which strongly influenced by the geographical origin grains, type milk used, manufacture technology applied. Until recently, kefir microbiota has been almost exclusively studied culture-dependent techniques. However, high-throughput sequencing, alongside omics approaches,...

10.3389/fmicb.2021.621069 article EN cc-by Frontiers in Microbiology 2021-01-28

Taxonomic studies were performed on some Streptococcus-like organisms isolated from naturally fermented Greek Kasseri cheese. By SDS-PAGE analysis of whole-cell proteins the group was found to be quite different Streptococcus thermophilus. Comparative 16S and 23S rRNA sequence analyses showed that isolates represent a new species within genus Streptococcus, where they are most closely related bovis cluster. On basis these phylogenetic results phenotypic differences, species, macedonicus, is...

10.1099/00207713-48-2-519 article EN International Journal of Systematic Bacteriology 1998-04-01

Streptococcus macedonicus ACA-DC 198, a strain isolated from Greek Kasseri cheese, produces food-grade lantibiotic named macedocin. Macedocin has molecular mass of 2,794.76 +/- 0.42 Da, as determined by electrospray spectrometry. Partial N-terminal sequence analysis revealed 22 amino acid residues that correspond with the lantibiotics SA-FF22 and SA-M49, both which were pathogen pyogenes. inhibits broad spectrum lactic bacteria, well several food spoilage pathogenic including Clostridium...

10.1128/aem.68.12.5891-5903.2002 article EN Applied and Environmental Microbiology 2002-11-26
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