Stephen DiFazio

ORCID: 0000-0003-4077-1590
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About
Contact & Profiles
Research Areas
  • Bioenergy crop production and management
  • Geophysics and Gravity Measurements
  • Ionosphere and magnetosphere dynamics
  • Solar and Space Plasma Dynamics
  • Genetic Mapping and Diversity in Plants and Animals
  • Genetic diversity and population structure
  • Chromosomal and Genetic Variations
  • Plant Reproductive Biology
  • Plant Molecular Biology Research
  • Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
  • Plant Gene Expression Analysis
  • Genetic and phenotypic traits in livestock
  • Yeasts and Rust Fungi Studies
  • Genetically Modified Organisms Research
  • Genomics and Phylogenetic Studies
  • Biofuel production and bioconversion
  • Plant and animal studies
  • Photosynthetic Processes and Mechanisms
  • Plant Pathogens and Fungal Diseases
  • Animal Genetics and Reproduction
  • CRISPR and Genetic Engineering
  • Plant biochemistry and biosynthesis
  • Fermentation and Sensory Analysis
  • Plant Stress Responses and Tolerance
  • Plant tissue culture and regeneration

West Virginia University
2014-2024

Morgantown High School
2017

University of Tennessee at Knoxville
2008

Northern Arizona University
2008

Oak Ridge National Laboratory
2002-2007

Umeå Plant Science Centre
2006

Umeå University
2006

Oregon State University
1996-2002

Gerald A. Tuskan Stephen DiFazio Stefan Jansson Jöerg Bohlmann Igor V. Grigoriev and 95 more Uffe Hellsten Nicholas H. Putnam Steven Ralph Stéphane Rombauts Asaf Salamov Jacqueline E. Schein Lieven Sterck Andrea Aerts R. R. Bhalerao Rishikesh P. Bhalerao Damien Blaudez Wout Boerjan Annick Brun Amy M. Brunner Victor Busov Malcolm M. Campbell John E. Carlson Michel Chalot Jarrod Chapman G.-L. Chen Dawn Cooper Pedro M. Coutinho Jérémy Couturier Sarah F. Covert Quentin Cronk Richard P. Cunningham John M. Davis Sven Degroeve Annabelle Déjardin Claude W. dePamphilis John C. Detter Bill Dirks Inna Dubchak Sébastien Duplessis Jürgen Ehlting B. E. Ellis Karla Gendler David Goodstein Michael Gribskov Jane Grimwood Andrew Groover Lee E. Gunter Björn Hamberger Berthold Heinze Ykä Helariutta Bernard Henrissat Dawn H. Nagel Robert A. Holt Wenjiang Huang Nurul Islam‐Faridi Steven J.M. Jones Matthew W. Jones-Rhoades Richard A. Jorgensen Chandrashekhar P. Joshi Jaakko Kangasjärvi Jan Karlsson Colin T. Kelleher Robert B. Kirkpatrick Matias Kirst Annegret Kohler Udaya C. Kalluri Frank W. Larimer Jim Leebens‐Mack Jean‐Charles Leplé Philip LoCascio Yonggen Lou Susan Lucas Francis Martin Barbara Montanini Carolyn A. Napoli David R. Nelson C. Dana Nelson Kaisa Nieminen Ove Nilsson V. Pereda G. F. Peter Ryan N. Philippe Gilles Pilate Alexandre Poliakov Jane Razumovskaya Paul Richardson Cécile Rinaldi Kermit Ritland Pierre Rouzé Dmitriy Ryaboy Jeremy Schmutz Jarmo Schrader Bo Segerman H. Shin Asim Siddiqui Fredrik Sterky Astrid Terry Chung‐Jui Tsai Ed Uberbacher Per Unneberg

We report the draft genome of black cottonwood tree, Populus trichocarpa . Integration shotgun sequence assembly with genetic mapping enabled chromosome-scale reconstruction genome. More than 45,000 putative protein-coding genes were identified. Analysis assembled revealed a whole-genome duplication event; about 8000 pairs duplicated from that event survived in A second, older is indistinguishably coincident divergence and Arabidopsis lineages. Nucleotide substitution, tandem gene...

10.1126/science.1128691 article EN Science 2006-09-15

The fungus Laccaria bicolor — seen in its above-ground fruiting body presence as the 'bicoloured deceiver' mushroom lives symbiotically on roots of trees. Its genome has now been sequenced, and key features characterized by transcript profiling. study throws light mechanism mycorrhizal symbiosis, union soil fungi that is vital important to plant productivity. And it will be keen interest evolutionary biologists for revelations about plant–fungus interactions shaping genomes over time....

10.1038/nature06556 article EN cc-by-nc-sa Nature 2008-03-01

An important step in the integration of ecology and genomics is progression from molecular studies relatively simple model systems to complex field systems. The recent availability sequenced genomes key plants leading a new understanding drivers community composition ecosystem processes. As genome sequences accumulate for species that form intimate associations nature, detailed view may emerge as how these cause changes among at nucleotide level. This advance could dramatically alter views...

10.1126/science.1153918 article EN Science 2008-04-24

The genus Populus consists of dioecious woody species with largely unknown genetic mechanisms for gender determination. We have discovered and genomic features in the peritelomeric region chromosome XIX that suggest this genome is process developing characteristics a sex chromosome. identified gender-associated locus consistently maps to region. Furthermore, comparison across multiple families reveals distorted segregation within intensively characterized using an F 1 interspecific cross...

10.1101/gr.7076308 article EN cc-by-nc Genome Research 2008-02-06

To assess the genetic control of biomass distribution in trees, phenotypic variation dry mass to stems, branches, leaves, coarse roots, and fine roots was examined two hybrid poplar (Populus trichocarpa Torr. & A. Gray (T) × Populus deltoides Bartr. ex Marsh. (D)) families grown under field conditions. Family 331 an inbred F 2 (TD TD) pedigree, whereas family 13 outbred backcross BC 1 D) pedigree. Fractional total whole-tree shoots during their establishment year averaged (±SD) 0.62 ±...

10.1139/x05-101 article EN Canadian Journal of Forest Research 2005-08-01

Comparative analysis of multiple angiosperm genomes has implicated gene duplication in the expansion and diversification many families. However, empirical data theory suggest that whole-genome small-scale events differ with respect to types genes preserved as duplicate pairs. We compared duplicates resulting from a recent whole genome set tandemly duplicated model forest tree Populus trichocarpa . used combination microarray expression analyses diverse tissues functional annotation assess...

10.1101/gr.125146.111 article EN Genome Research 2011-10-05

Summary Establishing links between phenotypes and molecular variants is of central importance to accelerate genetic improvement economically important plant species. Our work represents the first genome‐wide association study inherently complex currently poorly understood architecture industrially relevant wood traits. Here, we employed an Illumina Infinium 34 K single nucleotide polymorphism ( SNP ) genotyping array that generated 29 233 high‐quality s in c . 3500 broad‐based candidate...

10.1111/nph.12422 article EN New Phytologist 2013-07-26

QTL cloning for the discovery of genes underlying polygenic traits has historically been cumbersome in long-lived perennial plants like Populus. Linkage disequilibrium-based association mapping proposed as a tool, and recent advances high-throughput genotyping whole-genome resequencing enable marker saturation to levels sufficient with no priori candidate gene selection. Here, multiyear multienvironment evaluation cell wall phenotypes was conducted an interspecific P. trichocarpa x deltoides...

10.1186/s12864-015-1215-z article EN cc-by BMC Genomics 2015-01-23

Sex chromosomes have arisen independently in a wide variety of species, yet they share common characteristics, including the presence suppressed recombination surrounding sex determination loci. Mammalian contain multiple palindromic repeats across non-recombining region that show sequence conservation through gene conversion and genes are crucial for sexual reproduction. In plants, it is not clear if play role maintaining absence homologous recombination.Here we present first evidence large...

10.1186/s13059-020-1952-4 article EN cc-by Genome biology 2020-02-14

Summary Genome‐wide association studies (GWAS) have great promise for identifying the loci that contribute to adaptive variation, but complex genetic architecture of many quantitative traits presents a substantial challenge. We measured 14 morphological and physiological identified single nucleotide polymorphism (SNP)‐phenotype associations in Populus trichocarpa population distributed from California, USA British Columbia, Canada. used whole‐genome resequencing data 882 trees with more than...

10.1111/nph.15777 article EN publisher-specific-oa New Phytologist 2019-03-07

Summary Phylogenetic analysis is complicated by interspecific gene flow and the presence of shared ancestral polymorphisms, particularly those maintained balancing selection. In this study, we aimed to examine prevalence these factors during diversification Populus , a model tree genus in Northern Hemisphere. We constructed phylogenetic trees 29 taxa using 80 individuals based on re‐sequenced genomes. Our species analyses recovered four main clades consensus nuclear phylogenies, but conflict...

10.1111/nph.16215 article EN publisher-specific-oa New Phytologist 2019-09-24

Abstract Populus euphratica is well adapted to extreme desert environments and an important model species for elucidating the mechanisms of abiotic stress resistance in trees. The current assembly P. genome highly fragmented with many gaps errors, thereby impeding downstream applications. Here, we report improved chromosome‐level reference (v2.0) using single‐molecule sequencing chromosome conformation capture (Hi‐C) technologies. Relative previous genome, our represents a nearly 60‐fold...

10.1111/1755-0998.13142 article EN Molecular Ecology Resources 2020-02-08

Microsatellites or simple sequence repeats (SSRs) are highly polymorphic, codominant markers that have great value for the construction of genetic maps, comparative mapping, population surveys, and paternity analyses. Here, we report development testing a set SSR derived from shotgun sequencing Populus trichocarpa Torr. & A. Gray, nonenriched genomic DNA library, bacterial artificial chromosomes. Approximately 23% 1536 clones 48% 768 chromosome subclones contained an SSR. Of sequences...

10.1139/x03-283 article EN Canadian Journal of Forest Research 2004-01-01

Abstract: Trees, due to their long life‐span, have characteristics that distinguish them from annual, herbaceous plants. It is likely many of these properties are based on a tree‐specific genetic foundation. The U.S. Department Energy initiated genome‐sequencing project for Populus, model perennial plant. Through international collaboration and input the sequencing effort, annotated whole genome sequence Populus trichocarpa will be released public in early 2004. This genomic resource will,...

10.1055/s-2003-44715 article EN Plant Biology 2004-01-01

Abstract Genetic mapping of quantitative traits requires genotypic data for large numbers markers in many individuals. For such studies, the use single nucleotide polymorphism ( SNP ) genotyping arrays still offers most cost‐effective solution. Herein we report on design and performance a array Populus trichocarpa (black cottonwood). This was designed with s pre‐ascertained 34 wild accessions covering species latitudinal range. We adopted candidate gene approach to that resulted selection...

10.1111/1755-0998.12056 article EN Molecular Ecology Resources 2013-01-11
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