A5100624984- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Prenatal Screening and Diagnostics
- Chromosomal and Genetic Variations
- Genomic variations and chromosomal abnormalities
- Bioenergy crop production and management
- Plant Reproductive Biology
- Antibiotics Pharmacokinetics and Efficacy
- Synthesis and biological activity
- Cancer-related molecular mechanisms research
- Postharvest Quality and Shelf Life Management
- Plant Virus Research Studies
- Pharmacological Effects of Natural Compounds
- Traditional Chinese Medicine Studies
- Traditional Chinese Medicine Analysis
- Plant Disease Resistance and Genetics
- Natural Compounds in Disease Treatment
- Reproductive System and Pregnancy
- Genomics and Rare Diseases
- MicroRNA in disease regulation
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- CRISPR and Genetic Engineering
- Synthesis and Characterization of Heterocyclic Compounds
- Ectopic Pregnancy Diagnosis and Management
- Molecular Sensors and Ion Detection
University of Georgia
2021-2025
Nanjing Maternity and Child Health Care Hospital
2019-2024
Nanjing Medical University
2015-2024
Women's Hospital
2024
Shijiazhuang University
2011-2024
University of Manchester
2024
Ningxia University
2024
Anhui Academy of Agricultural Sciences
2024
Rice Research Institute
2024
Guangxi University
2023
Jianquan Liu and colleagues report the draft genome sequence of domestic yak, Bos grunniens. Their comparative analyses with low-altitude cattle provide insights into high-altitude adaptation in yak. Domestic yaks (Bos grunniens) meat other necessities for Tibetans living at high altitude on Qinghai-Tibetan Plateau adjacent regions. Comparison between yak closely related taurus) is informative studying animal to altitude. Here, we present a female generated using Illumina-based technology...
Sex chromosomes have arisen independently in a wide variety of species, yet they share common characteristics, including the presence suppressed recombination surrounding sex determination loci. Mammalian contain multiple palindromic repeats across non-recombining region that show sequence conservation through gene conversion and genes are crucial for sexual reproduction. In plants, it is not clear if play role maintaining absence homologous recombination.Here we present first evidence large...
Abstract Mitochondria, acting as the energy metabolism factory, participate in many key biological processes, including maintenance of sperm viability. Mitochondria-related microRNA (miRNA), encoded by nuclear genome or mitochondrial genome, may play an important regulatory role control function. To investigate potential mitochondria-related miRNAs asthenozoospermia, we adopted a strategy consisting initial screening TaqMan Low Density Array (TLDA) and further validation with quantitative...
Abstract Dioecy, the presence of separate sexes on distinct individuals, has evolved repeatedly in multiple plant lineages. However, specific mechanisms by which sex systems evolve and their commonalities among species remain poorly understood. With both XY ZW systems, family Salicaceae provides a system to uncover evolutionary forces driving chromosome turnovers. In this study, we performed genome-wide association study characterize determination two Populus species, P. euphratica alba. Our...
Abstract Mitochondria-related microRNAs (miRNAs) have recently emerged as key regulators of cell metabolism and can modulate mitochondrial fusion division. In order to investigate the roles mitochondria-related miRNAs played in obesity, we conducted comprehensive molecular analysis vitro vivo . Based on high-fat-diet (HFD) induced obese mice, found that hepatic function was markedly altered. Subsequently, evaluated expression levels selected miR-141-3p up-regulated strikingly HFD mice. To...
A series of quinoxaline derivatives were designed, synthesized and evaluated as antimicrobial agents against plant pathogenic bacteria fungi. Some these compounds exhibited significant antibacterial antifungal activities
A highly selective and divergent synthesis which enabled access to various complex compounds is attractive in organic medicinal chemistry. Herein, we developed an effective method for of substituted tetrahydroquinolines via Lewis base catalyzed switchable annulations Morita-Baylis-Hillman carbonates with activated olefins. The reaction displayed [4 + 2] or [3 catalyst substrate control, providing a diverse range architectures contained cyclopentenes three contiguous stereocenters bearing...
Abstract Background Salicaceae species have diverse sex determination systems and frequent chromosome turnovers. However, compared with poplars, the diversity of in willows is poorly understood, little known about evolutionary forces driving their turnover. Here, we characterized two Salix species, S. chaenomeloides arbutifolia , which an XY system on 7 15, respectively. Results Based assemblies regions, found that mechanism may underlying similarities both involving intact and/or partial...
As the focus for CRISPR/Cas-edited plants moves from proof-of-concept to real-world applications, precise gene manipulation will increasingly require concurrent multiplex editing polygenic traits. A common approach across multiple sites is design one guide RNA (gRNA) per target; however, this complicates construct assembly and increases possibility of off-target mutations. In study, we utilized gRNA target MYB186, a known positive trichome regulator, as well its paralogs MYB138 MYB38 at...
Twenty 1,4-pentadiene-3-one derivatives containing quinazolinone (W1–W20) were designed and synthesized. The bioactivity test results showed that some compounds had antifungal activities in vitro. W12 excellent against Sclerotinia sclerotiorum (S. sclerotiorum) Phomopsis sp., with EC50 values of 0.70 3.84 μg/mL, which are higher than those the control drug azoxystrobin at 8.15 17.25 μg/mL. In vivo activity tests carried out on oilseed rape kiwifruit. protective effect infected S. (91.7...
Abstract Background Facioscapulohumeral muscular dystrophy (FSHD) is a high-prevalence autosomal dominant neuromuscular disease characterized by significant clinical and genetic heterogeneity. Genetic diagnosis of FSHD remains challenge because it cannot be detected standard sequencing methods requires complex workflow. Methods We developed comprehensive detection method based on Oxford Nanopore Technologies (ONT) whole-genome sequencing. Using case–control design, we applied this procedure...
Abstract In Populus and many other tree species, photoassimilate sucrose diffuses down a concentration gradient via symplastically connected mesophyll cells to minor vein phloem for long‐distance transport. There is no evidence apoplastic phloem‐loading in . However, plasma membrane transporters (SUT1 SUT3) orthologous those associated with loading are expressed vascular tissues of poplar. While SUT3 functions import into developing xylem, the role SUT1 remains unclear. Here, we...
Congenital heart disease (CHD) is the most common birth defect. The prenatal diagnosis of fetal CHD completely dependent on ultrasound testing, but only ~40% can be detected. purpose this study to find good biomarkers in amniotic fluid (AF) detect second trimester, so as better manage group people and reduce harm fetus. Metabolites analysis were performed two separate sets. discovery set consisted 18 maternal AF samples 35 control samples, validation 53 114 samples. Untargeted metabolite...
Abstract Introduction Chromosomal aberrations are the most important etiological factors for birth defects. Optical genome mapping is a novel cytogenetic tool detecting broad range of chromosomal in single assay, but relevant clinical feasibility studies optical prenatal diagnosis limited. Material and methods We retrospectively performed analysis amniotic fluid samples from 34 fetuses with various indications detected through standard‐of‐care technologies, including karyotyping,...
Populus species play a foundational role in diverse ecosystems and are important renewable feedstocks for bioenergy bioproducts. Hybrid aspen tremula × P. alba INRA 717-1B4 is widely used transformation model tree functional genomics biotechnology research. As an outcrossing interspecific hybrid, its genome riddled with sequence polymorphisms which present challenge sequence-sensitive analyses. Here we report telomere-to-telomere this hybrid two chromosome-scale, haplotype-resolved...
Abstract Rice blast is a disastrous fungal disease affecting rice ( Oryza sativa L.) and undermines world food security. Different ecological conditions genetic backgrounds altered the effectiveness of resistance genes. Anhui (China) high‐incidence area has long been affected by blast. Despite chemical or other control methods blast, analyzing effects specific genes within region, identifying favorable (combinations), incorporating them into process new variety selection breeding programs...
The ages and sizes of a sex-determination region (SDR) are difficult to determine in non-model species. Due the lack recombination enrichment repetitive elements SDRs, quality assembly with short sequencing reads is universally low. Unique features present SDRs help provide clues about how established they evolve absence recombination. Several Populus species have been reported male heterogametic configuration sex (XX/XY system) mapped on chromosome 19, but exact location SDR has...
Background Infertility affects approximately 15% of couples worldwide with male infertility being responsible for 50% cases. Although accumulating evidence demonstrates the critical role X chromosome in spermatogenesis during last few decades, expression patterns and potential impact chromosome, together linked genes, on are less well understood. Methods We performed exome sequencing followed by a two-stage independent population validation 1333 non-obstructive azoospermia cases 1141 healthy...
Optical genome mapping (OGM) has been known as an all-in-one technology for chromosomal aberration detection. However, there are also aberrations beyond the detection range of OGM. This study aimed to report missed by OGM and analyze contributing factors. was performed taking both GRCh37 GRCh38 reference genomes. The results were analyzed in blinded fashion compared standard assays. Quality control (QC) metrics, sample types, genome, effective coverage classes locations then analyzed. In...