A5014351220- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Light effects on plants
- Plant Gene Expression Analysis
- Plant Parasitism and Resistance
- Porphyrin Metabolism and Disorders
- Plant and animal studies
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Mycorrhizal Fungi and Plant Interactions
- Aquatic Ecosystems and Phytoplankton Dynamics
- Antioxidant Activity and Oxidative Stress
- Biocrusts and Microbial Ecology
- Biochemical Acid Research Studies
- Environmental Toxicology and Ecotoxicology
- Genetic Mapping and Diversity in Plants and Animals
- Phytoplasmas and Hemiptera pathogens
- Chromosomal and Genetic Variations
- Bioenergy crop production and management
- Metalloenzymes and iron-sulfur proteins
- Plant Genetic and Mutation Studies
- Microbial metabolism and enzyme function
KWS Saat (Germany)
2015-2025
Oak Ridge National Laboratory
2012-2023
Humboldt-Universität zu Berlin
2003-2013
Max Planck Institute of Molecular Plant Physiology
2012
QTL cloning for the discovery of genes underlying polygenic traits has historically been cumbersome in long-lived perennial plants like Populus. Linkage disequilibrium-based association mapping proposed as a tool, and recent advances high-throughput genotyping whole-genome resequencing enable marker saturation to levels sufficient with no priori candidate gene selection. Here, multiyear multienvironment evaluation cell wall phenotypes was conducted an interspecific P. trichocarpa x deltoides...
5-Aminolevulinic acid (ALA) is the universal precursor for tetrapyrrole biosynthesis and synthesized in plants three enzymatic steps: ligation of glutamate (Glu) to tRNA(Glu) by glutamyl-tRNA synthetase, reduction activated Glu Glu-1-semialdehyde reductase (GluTR), transamination ALA 1-semialdehyde aminotransferase. formation controls metabolic flow into biosynthetic pathway. GluTR proposed be key regulatory enzyme that tightly controlled at transcriptional posttranslational levels. We...
Abstract Plant lignocellulosic biomass, i.e. secondary cell walls of plants, is a vital alternative source for bioenergy. However, the acetylation xylan in impedes conversion biomass to biofuels. Previous studies have shown that REDUCED WALL ACETYLATION (RWA) proteins are directly involved but regulatory mechanism RWAs not fully understood. In this study, we demonstrate overexpression Populus trichocarpa PtRWA-C gene increases level and lignin content S/G ratio, ultimately yielding poplar...
Summary Cyanobacteria are recognized as producers of a broad variety bioactive metabolites. Among these, the peptides synthesized by non‐ribosomal peptide synthetase pathway occur in high structural variability. One class cyanobacterial peptides, cyanopeptolins or micropeptins, have been shown to be strong inhibitors vertebrate serine proteases, like trypsin. In present study we screened extracts ten strains unicellular cyanobacterium Microcystis sp. for their potential inhibit trypsin‐like...
In photosynthetic organisms chlorophyll and heme biosynthesis is tightly regulated at various levels in response to environmental adaptation plant development. The formation of 5-aminolevulinic acid (ALA) the key regulatory step provides adequate amounts common precursor molecule for Mg Fe branches tetrapyrrole biosynthesis. Pathway control prevents accumulation metabolic intermediates avoids photo-oxidative damage. angiosperms reduction protochlorophyllide (Pchlide) chlorophyllide catalyzed...
During their first year of growth, overwintering biennial plants transport Suc through the phloem from photosynthetic source tissues to storage tissues. In second year, they mobilize carbon these fuel new growth and reproduction. However, both mechanisms driving this shift link reproductive remain unclear. vegetative sugar beet (
ABSTRACT Despite a high sucrose accumulation in its taproot vacuoles, sugar beet ( Beta vulgaris subsp. ) is sensitive to freezing. Earlier, taproot‐specific of raffinose was shown have beneficial effects on the freezing tolerance plant. However, synthesis and other oligosaccharides family depends availability myo ‐inositol. Since inositol inositol‐metabolising enzymes reside different organelles, functional metabolism depend transporters. We identified five homologues putative transporters...
Over the last few centuries, advancements in plant breeding have revolutionized agriculture, driving significant increases global food production. Polyploidy, increase chromosome copies, can positively affect performance and is assumed to played a critical role domestication of crop plants. Polyploidy thought be primarily caused by sperm that, due meiotic aberrations, deliver unreduced sets. We recently identified an alternative pathway polyploidization demonstrating that polyspermy,...
Abstract Low Chlorophyll Accumulation A (LCAA) antisense plants were obtained from a screen for genes whose partial down-regulation results in strong chlorophyll deficiency tobacco (Nicotiana tabacum). The LCAA mutants are affected plastid-localized protein of unknown function, which is conserved cyanobacteria and all photosynthetic eukaryotes. They suffer drastically reduced light-harvesting complex (LHC) contents, while the accumulation other complexes per leaf area less affected. As...
Receptor of activated C kinase1 (RACK1) is a versatile scaffold protein that binds to numerous proteins regulate diverse cellular pathways in mammals. In Arabidopsis (Arabidopsis thaliana), RACK1 has been shown plant hormone signaling, stress responses, and multiple processes growth development. However, little known about the molecular mechanism underlying these regulations. Here, we show an atypical serine (Ser)/threonine (Thr) kinase, WITH NO LYSINE8 (WNK8), phosphorylates RACK1. WNK8...
Sugar beet ( Beta vulgaris subsp. ) is the exclusive source of sugar in form sucrose temperate climate zones. grown there as an annual crop from spring to autumn because damaging effect freezing temperatures taproot tissue. A collection hybrid and non-hybrid cultivars was tested for winter survival rates tolerance. Three genotypes with either low or high were selected detailed study their response frost. These differed severity frost injury a defined inner region upper part taproot,...
The formation of 5-aminolevulinic acid (ALA) in tetrapyrrole biosynthesis is widely controlled by environmental and metabolic feedback cues that determine the influx into entire path. Because its central role as rate-limiting step, we hypothesised a potential ALA tetrapyrrole-mediated retrograde signalling exploited direct impact on nuclear gene expression (NGE) using two different approaches. Firstly, Arabidopsis gun1, hy1 (gun2), hy2 (gun3), gun4 mutants showing uncoupled NGE from...
Background Strigolactones are a new class of plant hormones that play key role in regulating shoot branching. Studies branching mutants Arabidopsis, pea, rice and petunia have identified several genes involved strigolactone biosynthesis or signaling pathway. In the model MORE AXILLARY GROWTH1 (MAX1), MAX2, MAX3 MAX4 four founding members pathway genes. However, little is known about woody perennial plants. Methodology/Principal Finding Here we report identification MAX homologues Populus...
Strigolactones (SLs) are a new class of plant hormones. In addition to acting as key inhibitor shoot branching, SLs stimulate seed germination root parasitic plants and promote hyphal branching colonization symbiotic arbuscular mycorrhizal fungi. They also regulate many other aspects growth development. At the transcription level, SL-regulated genes have been reported. However, nothing is known about proteome regulated by this A quantitative proteomics approach using an isobaric chemical...
DNA methylation is thought to influence the expression of genes, especially in response changing environmental conditions and developmental changes. Sugar beet (Beta vulgaris ssp. vulgaris), other biennial or perennial plants are inevitably exposed fluctuating temperatures throughout their lifecycle might even require such stimulus acquire floral competence. Therefore, as beets, need fine-tune epigenetic makeup ensure phenotypic plasticity towards while at same time steering essential...
Genetic engineering of plants that results in successful establishment new biochemical or regulatory pathways requires stable introduction one more genes into the plant genome. It might also be necessary to down-regulate turn off expression endogenous order reduce activity competing pathways. An established way knockdown gene is expressing a hairpin-RNAi construct, eventually leading degradation specifically targeted mRNA. Knockdown multiple do not share homologous sequences still...
This study focused on evaluating the effectiveness of seed treatments and different sugar beet varieties in controlling flea beetles (Chaetocnema tibialis) weevils (Asproparthenis punctiventris) Croatia. The field trials were conducted Vukovar-Sirmia County targeted developmental stages from BBCH 12 to 31. Although sowing was done within optimal period, no clear pattern between germination seeds susceptibility identified as results showed responses at development among three variants....