A5054272115- Plant Taxonomy and Phylogenetics
- Biological Activity of Diterpenoids and Biflavonoids
- Plant Molecular Biology Research
- Plant-derived Lignans Synthesis and Bioactivity
- Traditional and Medicinal Uses of Annonaceae
- Botany and Plant Ecology Studies
- Synthetic Organic Chemistry Methods
- Phytochemistry and Biological Activities
- Sesquiterpenes and Asteraceae Studies
- Bioactive Compounds and Antitumor Agents
- Chromosomal and Genetic Variations
- Natural product bioactivities and synthesis
- Phytochemistry and Bioactive Compounds
- Genetic diversity and population structure
- Genetic and phenotypic traits in livestock
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Animal Nutrition and Physiology
- Plant Virus Research Studies
- Evolution and Genetic Dynamics
- Marine Sponges and Natural Products
- Essential Oils and Antimicrobial Activity
- Soybean genetics and cultivation
- Genetic Mapping and Diversity in Plants and Animals
- Synthesis and biological activity
Universidad de Salamanca
1999-2024
Instituto de Investigación Biomédica de Salamanca
2021-2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2019-2022
Leibniz Institute of Plant Genetics and Crop Plant Research
2009-2019
Coburg University of Applied Sciences
2015
Government of Extremadura
2008-2013
Leibniz Institute for Neurobiology
2013
Centro de Investigación del Cáncer
2004
Consejo Superior de Investigaciones Científicas
2004
Universidad de Granada
2002-2004
Abstract We have compared the transcriptomic profiles of microdissected live ovules at four developmental stages between a diploid sexual and apomictic Boechera. sequenced >2 million SuperSAGE tags identified (1) heterochronic (n = 595) that demonstrated significantly different patterns expression across all stages, (2) stage-specific 577) were found in single stage differentially expressed ovules, (3) sex-specific 237) apomixis-specific 1106) but only one reproductive mode. Most...
Abstract For many years, parasitic B chromosomes have been considered genetically inert elements. Here we show the presence of ten protein-coding genes in chromosome grasshopper Eyprepocnemis plorans . Four these ( CIP2A, GTPB6, KIF20A , and MTG1 ) were complete whereas six remaining CKAP2, CAP-G, HYI, MYCB2, SLIT TOP2A truncated. Five MYCB2 significantly up-regulated B-carrying individuals, as expected if they actively transcribed from chromosome. This conclusion is supported by three...
Apomixis, a natural form of asexual seed production in plants, has evolved independently various taxa, and represents an important potential technology for agriculture. The switch to apomixis is based on de-regulation developmental pathways originally leading sexual formation. Hybridization polyploidy, both typical characteristics plants animals, are mechanisms that could trigger de-regulation. Here we show up-regulation alleles apomeiotic ovules mediated by genomic duplication, heterochrony...
Apomixis (asexual seed production) is characterized by meiotically unreduced egg cell production (apomeiosis) followed its parthenogenetic development into offspring that are genetic clones of the mother plant. Fertilization (i.e. pseudogamy) central important for a functional endosperm with balanced 2:1 maternal:paternal genome ratio. Here, we present APOLLO (for apomixis-linked locus) gene, an Aspartate Glutamate histidine exonuclease whose transcripts down-regulated in sexual ovules...
Significance Ecological-niche differentiation in diploid sexual–polyploid asexual complexes has been observed within and among many taxa, yet the relative contributions of reproductive system ploidy are not fully understood. Here, we assess niche characteristics sexual diploid, apomictic (asexual) triploid Boechera (Brassicaceae) lineages. We find strong evidence for widespread hybridization and, to a lesser degree, variation as factors that together overcome adaptive disadvantages (i.e.,...
During their first year of growth, overwintering biennial plants transport Suc through the phloem from photosynthetic source tissues to storage tissues. In second year, they mobilize carbon these fuel new growth and reproduction. However, both mechanisms driving this shift link reproductive remain unclear. vegetative sugar beet (
Cassava storage roots are among the most important root crops worldwide, and represent one of consumed staple foods in sub-Saharan Africa. The vegetatively propagated tropical shrub can form many starchy tuberous from its stem. These formed through activation secondary growth processes. However, underlying genetic regulation development is largely unknown. Here we report distinct structural transcriptional changes occurring during early phases development. A pronounced increase auxin-related...
Background Human malignant melanoma is a highly aggressive, heterogeneous and drug-resistant cancer. Due to high number of clones, harboring various mutations that affect key pathways, there an exceptional level phenotypic variation intratumor heterogeneity (ITH) in melanoma. This poses significant challenge personalized cancer medicine. Hitherto, it remains unclear what extent the affects immune microenvironment. Herein, we explore interaction between tumor host response cohort utilizing...
Abstract 28 cyclolignans, most of them derived from podophyllotoxin, have been evaluated for their antineoplastic and antiviral activities. They were subjected to screening against P‐388 murine leukemia, A‐549 human lung carcinoma, HT‐29 colon while assays performed on herpes simplex virus type I infecting fibroblasts monkey kidney (HSV/CV‐1) vesicular stomatitis hamster (VSV/BHK). A number substances active in both groups at concentrations below 1μM; deoxypodophyllotoxin ( 1 ) being the...
Podophyllotoxin and some of its derivatives are cyclolignans currently used for removing warts in the clinical treatment malign neoplasms. As such, they have been an objective scientific community decades, search more potent selective anticancer agents. Our interest chemoinduction drug selectivity led us to design preparation new podophyllotoxin by reaction podophyllic aldehyde with aliphatic, aromatic, heteroaromatic amines. Several resulting imines displayed a significant against human...
In apomictic Boechera spp., meiotic diplospory leads to the circumvention of meiosis and suppression recombination produce unreduced male female gametes (i.e. apomeiosis). Here, we have established an early flower developmental staging system performed microarray-based comparative gene expression analyses pollen mother cell stage in seven diploid sexual genotypes identify candidate factors for formation. We identified a transcript unique spp. called UPGRADE2 (BspUPG2), which is highly...
St. John's wort (Hypericum perforatum) is becoming an important model plant system for investigations into ecology, reproductive biology and pharmacology. This study investigates biogeographic variation population genetic structure reproduction in its ancestral (European) introduced (North America) ranges. Over 2000 individuals from 43 localities were analysed ploidy, microsatellite (19 loci) (flow cytometric seed screen). Most tetraploid (93 %), while lower frequencies of hexaploid (6...
Apomixis or asexual seed formation represents a potentially important agronomic trait whose introduction into crop plants could be an effective way to fix and perpetuate desirable genotype through successive generations. However, the gene regulatory pathways underlying apomixis remain unknown. In particular, potential function of microRNAs, which are known play crucial roles in many aspects plant growth development, remains determined with regards switch from sexual apomictic...
The most valuable lean cuts from Iberian pigs are the hams, forelegs, and loins, which yield high quality cured meat products. This study aimed to assess correlation between body composition measurements taken in vivo by ultrasonography those on carcass, were then used develop predictive models estimate weight of these cuts. Before slaughter, 241 weighed (slaughter BW) ultrasonically scanned. Ultrasound images collected at 3 locations: 10th intercostal space, caudal last rib image loin...
DNA methylation is thought to influence the expression of genes, especially in response changing environmental conditions and developmental changes. Sugar beet (Beta vulgaris ssp. vulgaris), other biennial or perennial plants are inevitably exposed fluctuating temperatures throughout their lifecycle might even require such stimulus acquire floral competence. Therefore, as beets, need fine-tune epigenetic makeup ensure phenotypic plasticity towards while at same time steering essential...
Parasites and hosts are involved in a continuous coevolutionary process leading to genetic changes both counterparts. To understand this process, it is necessary track host responses, one of which could be an increase sex recombination, such as proposed by the Red Queen hypothesis. In theoretical framework, inducible recombination hypothesis states that B-chromosomes (genome parasites prosper natural populations many living beings) elicit chiasma frequency favoured selection because...
Apomixis, a natural form of asexual seed production in plants, is considered to have great biotechnological potential for agriculture. It has been hypothesised that de-regulation the sexual developmental pathway could trigger apomictic reproduction. The genus Boechera represents an interesting model system understanding apomixis, having both and genotypes at diploid level. Quantitative qRT-PCR most extensively used method validating genome-wide gene expression analyses, but order obtain...
In asexual (apomictic) plants, the absence of meiosis and sex is expected to lead mutation accumulation. To compare accumulation in transcribed genomic regions sexual apomictic we performed a double-validated analysis copy number variation (CNV) on 10 biological replicates each diploid Boechera, using high-density (>700 K) custom microarray. The Boechera genome demonstrated higher levels depleted CNV, compared with enriched irrespective reproductive mode. Genome-wide patterns CNV revealed...