A5043468281- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Genetics, Bioinformatics, and Biomedical Research
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Cassava research and cyanide
- Plant Reproductive Biology
- Scientific Computing and Data Management
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- Bioinformatics and Genomic Networks
- Plant nutrient uptake and metabolism
- Cancer Genomics and Diagnostics
- Gene expression and cancer classification
- Transgenic Plants and Applications
- Microbial Metabolic Engineering and Bioproduction
- Banana Cultivation and Research
- Cancer-related molecular mechanisms research
- Biomedical and Engineering Education
- Animal Genetics and Reproduction
- Plant Micronutrient Interactions and Effects
- Seed Germination and Physiology
- MicroRNA in disease regulation
Institute of Biomedical Technologies
2011-2025
International Institute of Tropical Agriculture
2015-2025
National Research Council
2021-2024
International Institute of Tropical Agriculture
2015-2023
Istituto per le Tecnologie Didattiche
2007-2022
Medical Technologies (Czechia)
2010-2020
Instituto de Biología Molecular y Celular de Plantas
2010
Istituto Nazionale di Fisica Nucleare, Sezione di Bari
2010
University of California, Berkeley
1998-2002
ETH Zurich
1993-1998
Earth's life may have originated as self-replicating RNA, and it has been argued that RNA viruses viroid-like elements are remnants of such pre-cellular world. defined by linear genomes encoding an RNA-dependent polymerase (RdRp), whereas consist small, single-stranded, circular that, in some cases, encode paired self-cleaving ribozymes. Here we show the number candidate occurring geographically ecologically diverse niches is much higher than previously thought. We report amongst these...
Structural biology, homology modelling and rational drug design require accurate three-dimensional macromolecular coordinates. However, the coordinates in Protein Data Bank (PDB) have not all been obtained using latest experimental computational methods. In this study a method is presented for automated re-refinement of existing structure models PDB. A large-scale benchmark with 16 807 PDB entries showed that they can be improved terms fit to deposited X-ray data as well geometric quality....
How viroids, tiny non-protein-coding RNAs (~250-400 nt), incite disease is unclear. One hypothesis that viroid-derived small (vd-sRNAs; 21-24 nt) resulting from the host defensive response, via RNA silencing, may target for cleavage cell mRNAs and trigger a signal cascade, eventually leading to symptoms. Peach latent mosaic viroid (PLMVd), chloroplast-replicating viroid, particularly appropriate tackle this question because it induces an albinism (peach calico, PC) strictly associated with...
ABSTRACT The detection of viroid-derived small RNAs (vd-sRNAs) similar to the interfering (siRNAs, 21 24 nucleotides [nt]) in plants infected by nuclear-replicating members family Pospiviroidae (type species, Potato spindle tuber viroid [PSTVd]) indicates that they are inducers and targets RNA-silencing machinery their hosts. RNA-dependent RNA polymerase 6 (RDR6) catalyzes an amplification circuit producing double-stranded precursors secondary siRNAs. Recently, role RDR6 restricting systemic...
Viroids are circular, highly structured, non-protein-coding RNAs that, usurping cellular enzymes and escaping host defense mechanisms, able to replicate move through infected plants. Similarly viruses, viroid infections associated with the accumulation of viroid-derived 21-24 nt small (vd-sRNAs) typical features interfering characteristic RNA silencing, a sequence-specific mechanism involved in against invading nucleic acids regulation gene expression most eukaryotic organisms.To gain...
The identification of viroid-derived small RNAs (vd-sRNAs) 21 to 24 nucleotides (nt) in plants infected by viroids (infectious non-protein-coding just 250 400 nt) supports their targeting Dicer-like enzymes, the first host RNA-silencing barrier. However, whether viroids, like RNA viruses, are also targeted RNA-induced silencing complex (RISC) remains controversial. At RISC core is one Argonaute (AGO) protein that, guided endogenous or viral sRNAs, targets complementary RNAs. To examine AGO...
Abstract Plasmodesmata provide symplastic continuity linking individual plant cells. However, specialized cells may be isolated, either by the absence of plasmodesmata or down regulation cytoplasmic flux through these channels, resulting in formation domains. Maintenance domains essential for co- ordination growth and development. While center meristem divide slowly remain undifferentiated, on periphery more frequently respond to signals determining organ fate. Such were visualized within...
Northern-blot hybridization and low-scale sequencing have revealed that plants infected by viroids, non-protein-coding RNA replicons, accumulate 21-24 nt viroid-derived small RNAs (vd-sRNAs) similar to the interfering RNAs, hallmarks of silencing. These results strongly support viroids are elicitors targets silencing machinery their hosts. Low-scale sequencing, however, retrieves partial datasets may lead biased interpretations. To overcome this restraint we examined deep (Solexa-Illumina)...
The p53 gene family consists of the three genes p53, p63 and p73, which have polyhedral non-overlapping functions in pivotal cellular processes such as DNA synthesis repair, growth arrest, apoptosis, genome stability, angiogenesis, development differentiation. These encode sequence-specific nuclear transcription factors that recognise same responsive element (RE) their target genes. Their inactivation or aberrant expression may determine tumour progression developmental disease. discovery...
Cassava storage roots are among the most important root crops worldwide, and represent one of consumed staple foods in sub-Saharan Africa. The vegetatively propagated tropical shrub can form many starchy tuberous from its stem. These formed through activation secondary growth processes. However, underlying genetic regulation development is largely unknown. Here we report distinct structural transcriptional changes occurring during early phases development. A pronounced increase auxin-related...
Classical experiments in plant physiology showed that leaves are the source of signals control development flowers from shoot meristems. Additional physiological and genetic have indicated some molecules (e.g., gibberellins, cytokinins, sucrose) promote flowering mustards including Arabidopsis. These small hydrophilic likely to move apex symplastically via phloem and/or cell-to-cell movement through plasmodesmata. To analyze potential changes symplastic trafficking during induction...
Cassava is an important staple crop in sub-Saharan Africa, due to its high productivity even on nutrient poor soils. The metabolic characteristics underlying this are poorly understood including the mode of photosynthesis, reasons for rate extent source/sink limitation, impact environment, and variation between cultivars. Six commercial African cassava cultivars were grown a greenhouse Erlangen, Germany, field Ibadan, Nigeria. Source leaves, sink stems storage roots harvested during root...
ABSTRACT Rhizobia, bacterial symbionts of leguminous plants, produce lipo-chitin oligosaccharide (LCO) signal molecules that can induce nodule organogenesis in the cortex legume roots a host-specific way. The multi-unsaturated fatty acyl and O-acetyl moieties LCOs Rhizobium leguminosarum biovar viciae were shown to be essential for obtaining root induction Vicia sativa plants. We have used ballistic microtargeting as novel approach deliver derivatives nodulation inside V. sativa. This method...
Despite the importance of storage root (SR) organs for cassava and other crops yield, their developmental origin is poorly understood. Here we use multiple approaches to shed light on initial stages development demonstrating that SR fibrous roots (FR) follow different rhizogenic processes. Transcriptome analysis carried out collected before, during after bulking highlighted early specific activation a number functions essential swelling identified root-specific genes able effectively...
The African BioGenome Project (AfricaBP) is a Pan-African initiative aimed at improving food systems and biodiversity conservation through genomics while ensuring equitable data sharing benefits. Open Institute the knowledge exchange platform of AfricaBP which aims to bridge local gaps in bioinformatics enable infrastructural developments. In 2024, advanced this mission by organising 31 workshops that attracted more than 3500 registered attendees trained 380 researchers genomics,...
A method has been established that allows the targeted delivery of DNA‐carrying gold particles to vegetative shoot apical meristems cereal species. Meristems 8‐ 10‐day‐old seedlings wheat ( Triticum aestivum ), rice Oryza sativa ) and sorghum Sorghum bicolor were manually exposed by removal coleoptile first three four leaves. Using ballistic microtargeting, equal‐sized different diameters ranging from 1.0 2.0 µm propelled pulses compressed nitrogen 9 13 MPa. When accelerated MPa, 1.4 or...