A5022384768- Biofuel production and bioconversion
- Plant Pathogens and Fungal Diseases
- Phase Equilibria and Thermodynamics
- Microbial Metabolic Engineering and Bioproduction
- Plant Pathogenic Bacteria Studies
- Mycorrhizal Fungi and Plant Interactions
- Algal biology and biofuel production
- Plant Disease Resistance and Genetics
- Historical and socio-economic studies of Spain and related regions
- Catalysis and Oxidation Reactions
- Geological and Geophysical Studies Worldwide
- Enzyme Production and Characterization
- Bioenergy crop production and management
- Subcritical and Supercritical Water Processes
- Karst Systems and Hydrogeology
- Essential Oils and Antimicrobial Activity
- Archaeological and Historical Studies
- Plant Gene Expression Analysis
- Clostridium difficile and Clostridium perfringens research
- Free Radicals and Antioxidants
- Hydrology and Watershed Management Studies
- Plant Molecular Biology Research
- Advanced Cellulose Research Studies
- Gut microbiota and health
- Enzyme-mediated dye degradation
Oak Ridge National Laboratory
2013-2024
Bioenergy Life Science (United States)
2021-2022
Universidad Pablo de Olavide
2022
Government of the United States of America
2022
University of North Texas Health Science Center
2017
Oak Ridge Associated Universities
2017
University of North Texas
2017
Universidad de Cádiz
2004-2013
University of Sancti Spíritus José Martí Pérez
2000
CAB International
1998-1999
Economic feasibility and sustainability of lignocellulosic ethanol production requires the development robust microorganisms that can efficiently degrade convert plant biomass to ethanol. The anaerobic thermophilic bacterium Clostridium thermocellum is a candidate microorganism as it capable hydrolyzing cellulose fermenting hydrolysis products other metabolites. C. achieves efficient using multiprotein extracellular enzymatic complexes, termed cellulosomes.In this study, we used quantitative...
Clostridium thermocellum is a thermophilic, obligately anaerobic, Gram-positive bacterium that candidate microorganism for converting cellulosic biomass into ethanol through consolidated bioprocessing. Ethanol intolerance an important metric in terms of process economics, and tolerance has often been described as complex likely multigenic trait which gene interactions come play. Here, we resequence the genome ethanol-tolerant mutant, show tolerant phenotype primarily due to mutated...
The model bacterium Clostridium cellulolyticum efficiently degrades crystalline cellulose and hemicellulose, using cellulosomes to degrade lignocellulosic biomass. Although it imports ferments both pentose hexose sugars produce a mixture of ethanol, acetate, lactate, H2 CO2, the proportion ethanol is low, which impedes its use in consolidated bioprocessing for biofuels production. Therefore genetic engineering will likely be required improve yield. Plasmid transformation, random mutagenesis...
The ability of Clostridium thermocellum ATCC 27405 wild-type strain to hydrolyze cellulose and ferment the degradation products directly ethanol other metabolic byproducts makes it an attractive candidate for consolidated bioprocessing cellulosic biomass biofuels. In this study, whole-genome microarrays were used investigate expression C. mRNA during growth on crystalline in controlled replicate batch fermentations.A time-series analysis gene revealed changes transcript levels ~40% genes...
The recalcitrance of natural<italic>Populus</italic>variants was elucidated.
Abstract The extraction of carotenoids and chlorophylls using carbon dioxide modified with ethanol as a cosolvent is an alternative to solvent because it provides high‐speed process. In the study described here, carotenoid chlorophyll supercritical CO 2 + was explored freeze‐dried powders three microalgae ( Nannochloropsis gaditana , Synechococcus sp. Dunaliella salina ) raw materials. operation conditions were follows: pressures 200, 300, 400 500 bar, temperatures 40, 50 60°C. Analysis...
Long-lived perennial plants, with distinctive habits of inter-annual growth, defense, and physiology, are great economic ecological importance. However, some biological mechanisms resulting from genome duplication functional divergence genes in these systems remain poorly studied. Here, we discovered an association between a poplar (Populus trichocarpa) 5-enolpyruvylshikimate 3-phosphate synthase gene (PtrEPSP) lignin biosynthesis. Functional characterization PtrEPSP revealed that this...
The thermophilic anaerobe Clostridium thermocellum is a candidate consolidated bioprocessing (CBP) biocatalyst for cellulosic ethanol production. aim of this study was to investigate C. genes required ferment biomass substrates and conduct robust comparison DNA microarray RNA sequencing (RNA-seq) analytical platforms.C. ATCC 27405 fermentations were conducted with 5 g/L solid substrate loading either pretreated switchgrass or Populus. Quantitative saccharification inductively coupled plasma...
Higher ratios of syringyl-to-guaiacyl (S/G) lignin components Populus were shown to improve sugar release by enzymatic hydrolysis using commercial blends. Cellulolytic microbes are often robust biomass hydrolyzers and may offer cost advantages; however, it is unknown whether their activity can also be significantly influenced the ratio different monolignol types in biomass. Hydrolysis fermentation autoclaved, but otherwise not pretreated trichocarpa Clostridium thermocellum ATCC 27405 was...
Dynamic extraction of carotenoids from a marine strain Synechococcus sp. (Cyanophyceae) with supercritical CO2 (SC-CO2) was investigated regard to operation pressure and temperature effects on efficiency. Extraction yield (milligrams pigment per gram dry weight) for SC-CO2 compared the dimethylformamide (DMF). Carotenoids extracted were β-carotene (Ct), zeaxanthin (Z), β-cryptoxanthin (Cr), equinenone; chlorophyll poorly extracted, whereas myxoxanthophyll, another major carotenoid, not under...
A greater understanding of biosynthesis, signaling and regulatory pathways involved in determining stem growth secondary cell wall chemistry is important for enabling pathway engineering genetic optimization biomass properties. The present study describes a new functional role
Biomass analysis is a slow and tedious process not solely due to the long generation time for most plant species. Screening large numbers of variants various geno-, pheno-, chemotypes, whether naturally occurring or engineered in lab, has multiple challenges. Plant cell walls are complex, heterogeneous networks that difficult deconstruct analyze. Macroheterogeneity from tissue types, age, environmental factors makes representative sampling challenge natural variability generates significant...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTViscosity of triglycerides + alcohols from 278 to 313 KMiguel Rodriguez, Manuel Galan, Maria J. Munoz, and Ricardo MartinCite this: Chem. Eng. Data 1994, 39, 1, 102–105Publication Date (Print):January 1994Publication History Published online1 May 2002Published inissue 1 January 1994https://pubs.acs.org/doi/10.1021/je00013a027https://doi.org/10.1021/je00013a027research-articleACS PublicationsRequest reuse permissionsArticle...