A5077572585- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Bioenergy crop production and management
- Fungal and yeast genetics research
- Polysaccharides and Plant Cell Walls
- Fermentation and Sensory Analysis
- Microbial metabolism and enzyme function
- Food composition and properties
- Lignin and Wood Chemistry
- Plant tissue culture and regeneration
- Gene expression and cancer classification
- Plant Reproductive Biology
- Genetics, Aging, and Longevity in Model Organisms
- RNA regulation and disease
- Biochemical and biochemical processes
- Catalysis for Biomass Conversion
- Biomedical Text Mining and Ontologies
- Enzyme Catalysis and Immobilization
- Plant Gene Expression Analysis
- Microbial Metabolites in Food Biotechnology
- Topic Modeling
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
Oak Ridge National Laboratory
2014-2018
Oak Ridge Associated Universities
2017
University of Georgia
2016
University of Tennessee at Knoxville
2012
The recalcitrance of natural<italic>Populus</italic>variants was elucidated.
Long-lived perennial plants, with distinctive habits of inter-annual growth, defense, and physiology, are great economic ecological importance. However, some biological mechanisms resulting from genome duplication functional divergence genes in these systems remain poorly studied. Here, we discovered an association between a poplar (Populus trichocarpa) 5-enolpyruvylshikimate 3-phosphate synthase gene (PtrEPSP) lignin biosynthesis. Functional characterization PtrEPSP revealed that this...
Switchgrass is an abundant and dedicated bioenergy feedstock, however its inherent recalcitrance one of the economic hurdles for producing biofuels. The downregulation caffeic acid O-methyl transferase (COMT) gene in lignin pathway switchgrass reduced content S/G ratio, transgenic lines showed improved fermentation yield with Saccharomyces cerevisiae wild-type Clostridium thermocellum (ATCC 27405) comparison to switchgrass.Here we examine conversion COMT engineered evolved C. (M1570) strain....
Agave species can grow well in semi-arid marginal agricultural lands around the world. Selected are used largely for alcoholic beverage production Mexico. There expanding research efforts to use plentiful residues (bagasse) ethanol as manufacturing process only uses juice from central core of mature plants. Here, we investigate potential over a dozen species, including three cold regions United States, produce biofuels using whole plant. Ethanol was readily produced by Saccharomyces...
Lignocellulosic biomass continues to be investigated as a viable source for bioethanol production. However, the pretreatment process generates inhibitory compounds that impair growth and fermentation performance of microorganisms such Saccharomyces cerevisiae. Pinewood specifically has been shown challenging in obtaining industrially relevant ethanol titers. An industrial S. cerevisiae strain was subjected directed evolution adaptation pretreated pine resultant strains, GHP1 GHP4, exhibited...
Summary Transgenic Panicum virgatum L. silencing ( KD ) or overexpressing OE specific genes a small RNA GAUT 4 ‐ , mi 156‐ MYB 4‐ COMT and FPGS was grown in the field aerial tissue analysed for biofuel production traits. Clones representing independent transgenic lines were established senesced sampled after year 1 2 growth cycles. Biomass wall sugars, recalcitrance to enzymatic digestibility using separate hydrolysis fermentation. No correlation found between plant carbohydrate content...
Plant organ shape and size are established during growth by a predictable, controlled sequence of cell proliferation, differentiation, elongation. To understand the regulation coordination these processes, we studied temporal behavior epidermal cortex cells in Arabidopsis pedicels used computational modeling to analyze tissues. Pedicels offer multiple advantages for such study, as their is determinate, mostly one dimensional, epidermis differentiation uniform along proximodistal axis. Three...
Abstract Because cellulosic ethanol production remains cost‐prohibitive„ advances in consolidated bioprocessing (CBP) have been directed towards lifting this restriction. CBP reduces the need for added enzymes and can potentially slash costs through process integration. Clostridium thermocellum , a microorganism, organizes its multi‐enzyme complex ‐ stark contrast to fungal enzymes. Nonetheless, recalcitrance may limit extent of biomass deconstruction. Herein, six Populus were treated with...