A5012375126- Plant and Fungal Interactions Research
- Yeasts and Rust Fungi Studies
- Forest Insect Ecology and Management
- Forest ecology and management
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Horticultural and Viticultural Research
- Genetic diversity and population structure
- Bioenergy crop production and management
- Nuts composition and effects
- Ecology and Vegetation Dynamics Studies
- Fire effects on ecosystems
- Forest Ecology and Biodiversity Studies
- Genetic Mapping and Diversity in Plants and Animals
- Species Distribution and Climate Change
- Plant and animal studies
- Wheat and Barley Genetics and Pathology
- Magnetism in coordination complexes
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Metal complexes synthesis and properties
- Seedling growth and survival studies
- Plant Pathogens and Resistance
- Organometallic Complex Synthesis and Catalysis
- Plant Physiology and Cultivation Studies
Southern Research Station
2016-2025
US Forest Service
2008-2025
Southern Research Institute
2006-2025
University of Kentucky
2015-2021
United States Department of Agriculture
2010-2020
Louisiana Department of Natural Resources
2017
Oklahoma State University
2013-2017
Universidade Nova de Lisboa
2016
University of Southern Philippines Foundation
2007-2014
Oklahoma State University Oklahoma City
2013
We report the draft genome of black cottonwood tree, Populus trichocarpa . Integration shotgun sequence assembly with genetic mapping enabled chromosome-scale reconstruction genome. More than 45,000 putative protein-coding genes were identified. Analysis assembled revealed a whole-genome duplication event; about 8000 pairs duplicated from that event survived in A second, older is indistinguishably coincident divergence and Arabidopsis lineages. Nucleotide substitution, tandem gene...
Abstract Natural populations of forest trees exhibit striking phenotypic adaptations to diverse environmental gradients, thereby making them appealing subjects for the study genes underlying ecologically relevant phenotypes. Here, we use a genome-wide data set single nucleotide polymorphisms genotyped across 3059 functional patterns population structure and identify loci associated with aridity natural range loblolly pine (Pinus taeda L.). Overall structure, as inferred using principal...
Abstract Genetic association is a powerful method for dissecting complex adaptive traits due to (i) fine-scale mapping resulting from historical recombination, (ii) wide coverage of phenotypic and genotypic variation within single experiment, (iii) the simultaneous discovery loci alleles. In this article, genetic among nucleotide polymorphisms (58 SNPs) 20 wood- drought-related candidate genes an array wood property with evolutionary commercial importance, namely, earlywood latewood specific...
Background Genome evolution in the gymnosperm lineage of seed plants has given rise to many most complex and largest plant genomes, however elements involved are poorly understood. Methodology/Principal Findings Gymny is a previously undescribed retrotransposon family Pinus that related Athila Arabidopsis. dispersed throughout modern genome occupy physical space at least size Arabidopsis thaliana genome. In contrast described retroelements Pinus, was amplified or introduced after divergence...
The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting blight. pathogen, introduced from Asia, devastated American dentata) throughout its native range early in twentieth century. A highly informative genetic map of was constructed extend genomic studies Fagaceae and aid introgression blight genes into chestnut. Two mapping populations were established with three parents, 'Mahogany', 'Nanking', 'Vanuxem', totaling 337 progeny....
Summary Rapidly enhancing oleoresin production in conifer stems through genomic selection and genetic engineering may increase resistance to bark beetles terpenoid yield for liquid biofuels. We integrated association prediction analyses of flow (g 24 h −1 ) using 4854 single nucleotide polymorphisms ( SNP s) expressed genes within a pedigreed population loblolly pine P inus taeda that was clonally replicated at three sites the s outheastern U nited S tates. Additive variation 2 ≈ 0.12–0.30)...
The Japanese chestnut (Castanea crenata) carries resistance to Phytophthora cinnamomi, the destructive and widespread oomycete causing ink disease. European sativa), carrying little no disease resistance, is currently threatened by presence of pathogen in forests, orchards nurseries. Determining genetic basis P. cinnamomi for further selection molecular markers candidate genes, a prominent issue implementation marker assisted breeding programs resistance. In this study, first interspecific...
Loblolly pine (Pinus taeda L.) is one of the most widely planted and commercially important forest tree species in USA worldwide, an object intense genomic research. However, whole genome resequencing loblolly hampered by its large size complexity a lack good reference. As valid more feasible alternative, entire exome sequencing was hence employed to identify gene-associated single nucleotide polymorphisms (SNPs) genotype sampled trees. The exons were captured ADEPT2 association mapping...
A three-generation American chestnut × Chinese pedigree was used to construct a genetic linkage map for and investigate the control of resistance Endothia parasitica (chestnut blight fungus). DNA genotypes 241 polymorphic markers (eight isozymes, 17 restriction fragment length polymorphisms [RFLPs], 216 random amplified DNAs [RAPDs]) were assayed on an F 2 family consisting 102 individuals. Of these markers, 196 segregating as expected and, subsequently, primary mapping. Two 12 RFLPs, 170...
Previous loblolly pine (Pinus taeda L.) genetic linkage maps have been based on a variety of DNA polymorphisms, such as AFLPs, RAPDs, RFLPs, and ESTPs, but only few SSRs (simple sequence repeats), also known simple tandem repeats or microsatellites, mapped in P. taeda. The objective this study was to integrate large set SSR markers from sources published cDNA into composite map constructed two reference mapping pedigrees. A dense that incorporates loci will benefit complete genome...
Abstract Some evidence suggests that longleaf pine might be more tolerant of high winds than either slash (Pinus elliotii Englem.) or loblolly taeda L.). We studied wind damage to these three species in a common garden experiment southeast Mississippi following Hurricane Katrina, very large, Category 3 hurricane directly affected the stand August 2005. The experiment, factorial arrangement silvicultural treatments established 1960, included 120 plots 100 trees each, covering about 22 ha....
Rust fungi are a group of fungal pathogens that cause some the world's most destructive diseases trees and crops. A shared characteristic among rust is obligate biotrophy, inability to complete lifecycle without host. This dependence on host species likely affects patterns gene expansion, contraction, innovation within pathogen genomes. The establishment disease by biotrophic reliant upon effector proteins encoded in genome secreted from into host's cell apoplast or cells. study uses...
Restoration of foundation species, such as the American chestnut (Castanea dentata) that was devastated by an introduced fungus, can restore ecosystem function. Understanding both current distribution well biogeographic patterns is important for restoration planning. We used United States Department Agriculture Forest Service Inventory and Analysis data to quantify density C. dentata. then review literature concerning in Currently, 431 ± 30.2 million stems remain. The vast majority (360 22...
A karyotype and idiogram were prepared for slash pine (Pinus elliottii Engelm. var. elliottii, 2n = 2x 24) using 26 mitotic metaphase cells from root-tips of seedlings; each had 11 pairs long metacentric chromosomes one shorter pair submetacentric chromosomes. Fluorescent in situ hybridization showed seven with intercalary sites genes 18S-5.8S-25S rRNA(18S-25S rDNA) a paracentromeric site. Probes 5S rDNA localized major site on another chromosomes, whereas two minor occured other without...
Eight megagametophyte DNA samples from a single longleaf pine (Pinus palustris Mill.) tree were used to screen 576 oligonucleotide primers for random amplified polymorphic (RAPD) fragments. Primers amplifying repeatable fragments further characterized within sample of 72 megagametophytes the same tree. Fragments segregating in 1:1, present-to-absent, ratio classified as Mendelian markers and mapped using multipoint linkage analysis. The analysis revealed 16 groups at least three markers,...
Loblolly pine (LP; Pinus taeda L.) is the most economically important tree in U.S. and a cornerstone species southeastern forests. However, genomics research on LP other conifers has lagged behind studies flowering plants due, part, to large size of conifer genomes. As means accelerate genome research, we constructed BAC library for genotype 7-56. The consists 1,824,768 individually-archived clones making it largest single date, mean insert 96 kb, affords 7.6X coverage 21.7 Gb genome. To...
A consensus genetic map for Pinus taeda (loblolly pine) and elliottii (slash was constructed by merging three previously published P. maps with a from pseudo-backcross between taeda. The positioned 3856 markers via genotyping of 1251 individuals four pedigrees. It is the densest linkage conifer to date. Average marker spacing 0.6 cM total length 2305 cM. Functional predictions mapped genes were improved aligning expressed sequence tags used discovery full-length transcripts. Alignments...