A5013845406- Mycorrhizal Fungi and Plant Interactions
- Heavy metals in environment
- Geochemistry and Elemental Analysis
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Microbial Community Ecology and Physiology
- Bioenergy crop production and management
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Aluminum toxicity and tolerance in plants and animals
- Chromium effects and bioremediation
- Forest Ecology and Biodiversity Studies
- Trace Elements in Health
- Biocrusts and Microbial Ecology
- Lichen and fungal ecology
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Plant Micronutrient Interactions and Effects
- Geology and Paleoclimatology Research
- Algal biology and biofuel production
- Botany and Plant Ecology Studies
- African Botany and Ecology Studies
- Plant Parasitism and Resistance
- Extraction and Separation Processes
Université de Lorraine
2016-2025
Laboratoire Interdisciplinaire des Environnements Continentaux
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Interactions Arbres-Microorganismes
2005-2013
University of Cologne
2011
Institut National de la Recherche Agronomique
2000-2009
Oak Ridge National Laboratory
2006
University of Tennessee at Knoxville
2006
West Virginia University
2006
Umeå Plant Science Centre
2006
We report the draft genome of black cottonwood tree, Populus trichocarpa . Integration shotgun sequence assembly with genetic mapping enabled chromosome-scale reconstruction genome. More than 45,000 putative protein-coding genes were identified. Analysis assembled revealed a whole-genome duplication event; about 8000 pairs duplicated from that event survived in A second, older is indistinguishably coincident divergence and Arabidopsis lineages. Nucleotide substitution, tandem gene...
The fungus Laccaria bicolor — seen in its above-ground fruiting body presence as the 'bicoloured deceiver' mushroom lives symbiotically on roots of trees. Its genome has now been sequenced, and key features characterized by transcript profiling. study throws light mechanism mycorrhizal symbiosis, union soil fungi that is vital important to plant productivity. And it will be keen interest evolutionary biologists for revelations about plant–fungus interactions shaping genomes over time....
Abstract Background The Cation Diffusion Facilitator (CDF) family is a ubiquitous of heavy metal transporters. Much interest in this has focused on implications for human health and bioremediation. In work broad phylogenetic study been undertaken which, considered the context functional characteristics some fully characterised CDF transporters, aimed at identifying molecular determinants substrate selectivity suggesting specificity newly identified Results Representative members from all...
Organization of proteins into complexes is crucial for many cellular functions. However, most proteomic approaches primarily detect protein interactions soluble but are less suitable membrane-associated complexes. Here we describe a mating-based split ubiquitin system (mbSUS) systematic identification between membrane as well and proteins. mbSUS allows in vivo cloning PCR products vector set, detection via mating, regulated expression baits, improved selection interacting Cloning simplified...
Manganese toxicity is a major problem for plant growth in acidic soils, but cellular mechanisms that facilitate such conditions have not been clearly delineated. Established counter metal plants involve chelation and cytoplasmic export of the across plasma or vacuolar membranes out cell sequestered into large organelle, respectively. We report here expression Arabidopsis poplar MTP11 cation diffusion facilitators manganese-hypersensitive yeast mutant restores manganese tolerance to wild-type...
Cadmium uptake and subcellular compartmentation in the ectomycorrhizal fungus Paxillus involutus were investigated using radiotracer flux analyses. Concentration-dependent Cd2+-uptake kinetics characterized by a smooth, non-saturating curve that could be dissected into linear saturable components. The linear-uptake kinetic component was interpreted as representing binding of Cd to apoplastic components, whereas remaining result carrier-mediated transport across plasma membrane....
Cation diffusion facilitator (CDF) proteins are a recently discovered family of cation efflux transporters that might play an essential role in metal homeostasis and tolerance. Here, we describe the identification, characterization, localization PtdMTP1, member CDF from hybrid poplar Populus trichocarpa × deltoides. PtdMTP1 is expressed constitutively ubiquitously, although at low levels. Heterologous expression yeast showed was able to complement hypersensitivity mutant strains Zn but not...
• Ammonium and nitrate are the prevalent nitrogen sources for growth development of higher plants. Here, we report on characterization ammonium transporter (AMT) family in perennial species Populus trichocarpa. In silico analysis expression AMT genes from poplar was performed. addition, AMT1;2 AMT1;6 function studied detail by heterologous yeast. The P. trichocarpa genome contains 14 putative AMTs, which is more than twice number AMTs Arabidopsis. roots, high-affinity strongly increased upon...
Gene networks involved in inorganic phosphate (Pi) acquisition and homeostasis woody perennial species able to form mycorrhizal symbioses are poorly known. Here, we describe the features of 12 genes coding for Pi transporters Pht1 family poplar (Populus trichocarpa). Individual play distinct roles acquiring translocating different tissues nonmycorrhizal during growth conditions developmental stages. starvation triggered up-regulation most members family, especially PtPT9 PtPT11. PtPT12...
Numerous studies address the distribution and diversity of dark septate endophytes (DSEs) in literature, but little is known about their ecological role effect on host plants, especially metal-polluted soils. Seven DSE strains belonging to Cadophora, Leptodontidium, Phialophora Phialocephala were isolated from roots poplar trees sites. All developed a wide range carbohydrates, including cell-wall-related compounds. The evenly colonized birch, eucalyptus ryegrass re-synthesis experiments....
• In an effort to understand processes that are related heavy metal sequestration, we characterized six metallothionein genes (PtdMTs) in the hybrid cottonwood, Populus trichocarpa × deltoides. cDNA microarrays and reverse transcriptase-polymerase chain reaction were used examine PtdMT expression poplar tissues. They displayed differential gene patterns, which may be associated with diverse roles functions PtdMTs have coping particular developmental (e.g. root development leaf senescence)...
Abstract Rare earth elements (REEs) are now considered emerging pollutants in the environment. Phytolacca americana , an REE hyperaccumulating plant, has been proposed for remediation of REE-contaminated soils. However, there is no REE-related information other species. Here, we examined five species ( P. acinosa clavigera bogotensis and icosandra ) their response to REEs. accumulation fractionation traits both occurred on same order magnitude among Heavy REEs were preferentially transferred...
High-throughput sequencing has become a prominent tool to assess plant-associated microbial diversity. Still, some technical challenges remain in characterising these communities, notably due plant and fungal DNA co-amplification. Fungal-specific primers, Peptide Nucleic Acid (PNA) clamps, or adjusting PCR conditions are approaches limit contamination. However, systematic comparison of factors their interactions, which could contamination the study mycobiota, is still lacking. Here, three...
• Metallothioneins are small polypeptides involved in metal tolerance of many eukaryotes. Here we characterized the Pimt1 gene, coding for a metallothionein from ectomycorrhizal fungus Paxillus involutus. Expression P. involutus under stress conditions was measured by northern blot and RT-PCR analyses. The full-length cDNA used to perform functional complementation yeast mutant strains agrotransformation Hebeloma cylindrosporum. Heterologous expression showed that PiMT1 able complement...
Heavy metals are pollutants which affect all organisms. Since a small number of eukaryotes have been investigated with respect to metal resistance, we hypothesize that many genes control this phenomenon remain be identified. This was tested by screening soil eukaryotic metatranscriptomes encompass RNA from organisms belonging the main phyla. Soil-extracted polyadenylated mRNAs were converted into cDNAs and 35 them selected for their ability rescue (Cd or Zn) sensitive phenotype yeast...