A5038540226- Biofuel production and bioconversion
- Polysaccharides and Plant Cell Walls
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Bioenergy crop production and management
- Plant Gene Expression Analysis
- Microbial Metabolic Engineering and Bioproduction
- Light effects on plants
- Plant Molecular Biology Research
- Lignin and Wood Chemistry
- Food composition and properties
- Plant nutrient uptake and metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Soybean genetics and cultivation
- Enzyme Production and Characterization
- Plant tissue culture and regeneration
- Mycotoxins in Agriculture and Food
- Essential Oils and Antimicrobial Activity
- Plant Surface Properties and Treatments
- Phytochemistry and Bioactivity Studies
- Anaerobic Digestion and Biogas Production
- CRISPR and Genetic Engineering
- Seed and Plant Biochemistry
- Groundwater flow and contamination studies
- Enzyme-mediated dye degradation
University of Georgia
2015-2024
Jawaharlal Nehru University
2004-2023
Oak Ridge National Laboratory
2013-2019
Bioenergy Life Science (United States)
2019
Swedish University of Agricultural Sciences
2014
International Centre for Genetic Engineering and Biotechnology
2012
University of Illinois Urbana-Champaign
2012
Indian Institute of Water Management
2006
Utkal University
2000-2002
The inherent recalcitrance of woody bioenergy feedstocks is a major challenge for their use as source second-generation biofuel. Secondary cell walls that constitute the majority hardwood biomass are rich in cellulose, xylan, and lignin. interactions among these polymers prevent facile accessibility deconstruction by enzymes chemicals. Plant can with minimal pretreatment be degraded into sugars required to produce renewable biofuels cost-effective manner. GAUT12/IRX8 putative...
Abstract Chlorophyll b is synthesized by the oxidation of a methyl group on B ring tetrapyrrole molecule to formyl chlorophyllide oxygenase (CAO). The full-length CAO from Arabidopsis (Arabidopsis thaliana) was overexpressed in tobacco (Nicotiana tabacum) that grows well at light intensities much higher than those tolerated Arabidopsis. This resulted an increased synthesis glutamate semialdehyde, 5-aminolevulinic acid, magnesium-porphyrins, and chlorophylls. Overexpression chlorophyll...
Summary Genome‐wide association studies (GWAS) have great promise for identifying the loci that contribute to adaptive variation, but complex genetic architecture of many quantitative traits presents a substantial challenge. We measured 14 morphological and physiological identified single nucleotide polymorphism (SNP)‐phenotype associations in Populus trichocarpa population distributed from California, USA British Columbia, Canada. used whole‐genome resequencing data 882 trees with more than...
The recalcitrance of cellulosic biomass is widely recognized as a key barrier to cost-effective biological processing fuels and chemicals, but the relative impacts physical, chemical genetic interventions improve singly in combination have yet be evaluated systematically. Solubilization plant cell walls can enhanced by non-biological augmentation including physical cotreatment thermochemical pretreatment, choice biocatalyst, feedstock, engineering plants, choosing feedstocks that are less...
The three major components of plant biomass, cellulose, hemicellulose and lignin, are highly recalcitrant deconstruction involves thermal chemical pretreatment. Microbial conversion is a possible solution, but few anaerobic microbes utilize both cellulose none known to solubilize lignin. Herein, we show that the majority (85%) insoluble switchgrass biomass had not been previously chemically treated was degraded at 78 °C by bacterium Caldicellulosiruptor bescii. Remarkably,...
Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they important sources of renewable biomass for producing energy green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers highly cross-linked not easily accessible to enzymes We show that aspen pectate lyase PL1-27, which degrades homogalacturonan is expressed at the onset secondary wall formation, can increase solubility matrix polysaccharides....
A major obstacle, and perhaps the most important economic barrier to effective use of plant biomass for production fuels, chemicals, bioproducts, is our current lack knowledge how efficiently effectively deconstruct wall polymers their subsequent as feedstocks. Plants represent desired source renewable energy hydrocarbons because they fix CO2, making carbon neutral. Their structure, however, a deconstruction, this often referred recalcitrance. Members bacterial genus Caldicellulosiruptor...
The development of fast-growing hardwood trees as a source lignocellulosic biomass for biofuel and biomaterial production requires thorough understanding the plant cell wall structure function that underlie inherent recalcitrance properties woody biomass. Downregulation GAUT12.1 in Populus deltoides was recently reported to result improved saccharification, growth, yield. To further understand here we report effects P. trichocarpa overexpression deltoides.Increasing transcript expression by...
Knockdown (KD) expression of
Plant growth and grain filling are the key agronomical traits for weight yield of rice. The continuous improvement in rice is required a future sustainable global economy food security. heterotrimeric G protein complex containing canonical α subunit (RGA1) couples extracellular signals perceived by receptors to modulate cell function including plant development weight. We hypothesized that, besides RGA1, three atypical, extra-large GTP-binding (XLG) subunits also regulate panicle...
Abstract Background Secondary cell wall holds considerable potential as it has gained immense momentum to replace the lignocellulosic feedstock into fuels. Lignin one of components secondary tightly polysaccharides thereby enhancing recalcitrance and complexity in biomass. Laccases (LAC) peroxidases (PRX) are major phenyl-oxidases playing key functions during polymerization monolignols lignin. Yet, laccase gene families remained largely unknown. Hence, objective this conducted study is...
One of the major barriers to development lignocellulosic feedstocks is recalcitrance plant cell walls deconstruction and saccharification. Recalcitrance can be reduced by targeting genes involved in wall biosynthesis, but this have unintended consequences that compromise agronomic performance trees under field conditions. Here we report results a trial fourteen distinct transgenic Populus deltoides lines had previously demonstrated without yield penalties greenhouse Survival productivity...
Switchgrass (Panicum virgatum L.) is a leading lignocellulosic bioenergy feedstock. Cellulose major component of the plant cell walls and primary substrate for saccharification. Accessibility cellulose to enzymatic breakdown into fermentable sugars limited by presence lignin in wall. In this study, putatively novel switchgrass secondary wall synthase PvCesA4 PvCesA6 genes were identified their functional role synthesis composition was examined overexpression knockdown individual switchgrass....
Summary Transgenic Panicum virgatum L. silencing ( KD ) or overexpressing OE specific genes a small RNA GAUT 4 ‐ , mi 156‐ MYB 4‐ COMT and FPGS was grown in the field aerial tissue analysed for biofuel production traits. Clones representing independent transgenic lines were established senesced sampled after year 1 2 growth cycles. Biomass wall sugars, recalcitrance to enzymatic digestibility using separate hydrolysis fermentation. No correlation found between plant carbohydrate content...
The major cell wall pectic glycan homogalacturonan (HG) is crucial for plant growth, development, and reproduction. HG synthesis occurs in the Golgi catalyzed by members of galacturonosyltransferase (GAUT) family with GAUT1 being archetypal best studied member. In Arabidopsis suspension culture cells tobacco leaves, localization has been shown to require protein-protein interactions its homolog GAUT7. Here we show that pollen tubes GAUT5 GAUT6, homologs GAUT7, also target apparatus. Pollen...
The effective use of plant biomass for biofuel and bioproduct production requires a comprehensive glycosyl residue composition analysis to understand the different cell wall polysaccharides present in sources. Here we compared four methods side-by-side their ability measure neutral acidic sugar walls from herbaceous, grass, woody model plants bioenergy feedstocks. Arabidopsis, Populus, rice, switchgrass leaf walls, as well Populus wood, rice stems, tillers, were analyzed by (1) gas...
The mission of the BioEnergy Science Center (BESC) was to enable efficient lignocellulosic-based biofuel production. One BESC goal decrease poplar and switchgrass biomass recalcitrance conversion while not affecting plant growth. A transformation pipeline (TP), express transgenes or transgene fragments (constructs) in these feedstocks with understanding decreasing recalcitrance, considered essential for this goal. Centralized data storage access by members later public also essential.A...
Tree nuts have been associated with several foodborne outbreaks and recalls in the U.S. While thermal decontamination methods can inactivate pathogens, they come challenges of varying severity on product quality. Thus, this study evaluated effects nonthermal intense pulsed light (IPL) Salmonella Typhimurium inactivation quality attributes pecan halves. The level microbial reduction increased an increase treatment time from 10 to 40 s decreased distance (8.28–13.36 cm) source. Both treatments...
Lignin–carbohydrate complexes (LCCs) form through interactions of lignin with plant cell wall polysaccharides and are thought to be a significant source biomass recalcitrance. In this work, we investigated LCCs formed between pectin homogalacturonan (HG). The structural changes in HG deficient transgenic switchgrass (GAUT4-knockdown, GAUT4-KD) after hot water pretreatment were compared wild-type plants using small-angle neutron scattering (SANS), which showed that there ∼2.2-fold more...