A5080063499- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Dialysis and Renal Disease Management
- Skin and Cellular Biology Research
- Chemical Synthesis and Analysis
- Chronic Kidney Disease and Diabetes
- Monoclonal and Polyclonal Antibodies Research
- Adipose Tissue and Metabolism
- Enzyme Structure and Function
- Asthma and respiratory diseases
- Biochemical and Molecular Research
- Eicosanoids and Hypertension Pharmacology
- Chemokine receptors and signaling
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Inflammatory mediators and NSAID effects
- Ferroelectric and Piezoelectric Materials
- Wnt/β-catenin signaling in development and cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Iron Metabolism and Disorders
- Amphibian and Reptile Biology
- Multiferroics and related materials
- Renal Diseases and Glomerulopathies
- Erythropoietin and Anemia Treatment
- Vascular Anomalies and Treatments
Colorado School of Mines
2021-2022
North Carolina State University
2010
University of Illinois Urbana-Champaign
2010
Howell Laboratories (United States)
2010
University of Georgia
2010
Louisiana State University
2007-2010
Mosaiques Diagnostics and Therapeutics (Germany)
2004-2008
Medizinische Hochschule Hannover
2002-2005
University of Cambridge
2005
National High Magnetic Field Laboratory
2005
Abstract CE‐MS is a successful proteomic platform for the definition of biomarkers in different body fluids. Besides biomarker defining experimental parameters, CE migration time and molecular weight, especially biomarker's sequence identity an indispensable cornerstone deeper insights into pathophysiological pathways diseases or made‐to‐measure therapeutic drug design. Therefore, this report presents detailed discussion peptide sequencing platforms consisting high performance separation...
Renal disease in patients with Type II diabetes is the leading cause of terminal renal failure and a major healthcare problem. Hence early identification prone to develop this complication important. Diabetic damage should be reflected by change urinary polypeptide excretion at very stage. To analyse these changes, we used an online combination CE/MS (capillary electrophoresis coupled MS), allowing fast accurate evaluation up 2000 polypeptides urine. Employing technology, have examined urine...
Angiotensin II (Ang II) type 1 receptor (AT ) antagonists such as losartan (LOS) are widely used for the treatment of hypertension and elicit antiinflammatory antiaggregatory in vitro patients, although underlying mechanism unclear. Following computer-based molecule similarity, we proposed that on cytochrome-P450 degradation, LOS metabolite EXP3179 is generated, which shows homology to indomethacin, a cyclooxygenase inhibitor with properties. Subsequently, serum-levels were determined 8...
We describe the use of capillary electrophoresis (CE) coupled with mass spectrometry (MS) to identify single polypeptides and patterns specific for prostate cancer (CaP) in human urine. Using improved sample preparation methods that enable enhanced comparability between different samples, we examined samples from 47 patients who underwent biopsy. Of this group, 21 had benign pathology 26 CaP, these were used define potential biomarkers, which allow discrimination two states. In addition,...
This study investigates proteomic analysis of urinary samples as a non-invasive method to detect acute rejection renal allografts. Capillary electrophoresis coupled mass spectrometry (CE-MS) was used analyze in 19 patients with different grades subclinical or clinical (BANFF Ia IIb), 10 tract infection and 29 without evidence infection. A distinct polypeptide pattern identified 16 out 17 cases tubolointerstitial rejection, but absent two vascular rejection. Urinary resulted that allowed...
Abstract We report on our results using capillary electrophoresis coupled to mass spectrometry (CE‐MS) examine human bodyfluids. To demonstrate the versatility of this approach, data two different bodyfluids, urine and cerebrospinal fluid, are shown. CE‐MS analysis enables identification a series polypeptides which serve as biomarkers for variety renal diseases. The utilized generate disease‐specific polypeptide patterns. Diagnosis these diseases is possible based patters. Further, due high...
Background. Uraemic toxicity results in the dysfunction of many organ systems, provoking an increase morbidity and mortality. To date, only ∼90 uraemic retention solutes have been described. examine unknown substances thoroughly, identification as compounds possible ultrafiltrate and/or plasma patients would lead to a less biased definition process compared with what is proposed today.
Several skin diseases and atopic disorders including Netherton syndrome dermatitis have been associated with mutations deviations of expression SPINK5, the gene encoding human 15-domain serine proteinase inhibitor LEKTI. The biochemical mechanisms underlying this phenomenon not yet fully clarified.To identify target proteinases LEKTI important for processes desquamation inflammation which will enable development specific drugs.The inhibitory activities domains 6 15 were tested on a number...
Here we describe a mass spectrometry (MS) approach for biomarker discovery and structural characterization, based on both top-down bottom-up analyses. Capillary electrophoresis (CE) coupled to electrospray ionization (ESI) time-of-flight (TOF) MS serves separate mass-measure the thousands of polypeptides contained in human urine. Statistical analysis differences between healthy control samples patients with focal-segmental glomerulosclerosis, membranous glomerulonephritis, minimal change...
To determine the efficacy of 21-day therapy with azithromycin and doxycycline in treatment experimental infection Chlamydophila psittaci cockatiels (Nymphicus hollandicus), 30 birds randomly assigned to 3 groups 1 control group were inoculated C by combined intranasal ocular routes. Morbidity, mortality, results polymerase chain reaction testing confirmed that was successful. Birds (n = 8) received at 40 mg/kg PO q48h for 21 days; 2 8), 35 q24h 45 and, 4 (controls; n 6), no treatment. Six...
Evidence indicates that oxidative stress is present in dialysis patients, and associated with vitamin C deficiency. Limited data are available regarding the effects of supplementation on inflammation markers these patients. Moreover, there no plasma polypeptide fingerprints by proteome analysis before after supplementation. Therefore, we analyzed samples from a prospective, randomized, open-labeled trial to assess oral (250 mg three times per week), define pattern hemodialysis Our results...
Abstract CCR3 is responsible for tissue infiltration of eosinophils, basophils, mast cells, and Th2 particularly in allergic diseases. In this context, has emerged as a target the treatment asthma. It well known that N-terminal domain chemokines crucial receptor binding and, particular, its activation. Based on background, we investigated number N-terminally truncated or modified peptides derived from chemokine CCL14/hemofiltrate CC chemokine-1 their ability to modulate activity CCR3. Among...
Both key enzymes for the glyoxylate cycle, isocitrate lyase (EC 4.1.3.1) and malate synthase 4.1.3.2), were purified characterized from thermoacidophilic crenarchaeon Sulfolobus acidocaldarius . Whereas former enzyme was copurified with aconitase, latter could be enriched to apparent homogeneity. Amino acid sequencing of three internal peptides revealed presence highly conserved residues. With respect cofactor requirement quarternary structure crenarchaeal might represent a novel type this...
Green iguanas (Iguana iguana) have been documented to become aggressive during their breeding season and the menstrual cycles of human female caregivers. Because large size, these reptiles potential inflict severe bite wounds. Identifying a method control aggression in animals is needed minimize likelihood injury handlers. The purpose this study was evaluate effect mammalian leuprolide acetate on testosterone levels captive, intact male green iguanas. Eighteen adult were used for study....
A male mallard duck (Anas platyrhynchos) presented for examination acute respiratory distress and lethargy. The had experienced recurrent episodes of since being attacked by a raccoon the previous year, resulting in neck lacerations. Diagnostic tests, including complete blood count, plasma biochemical analysis, radiography, tracheoscopy, revealed collapsed trachea. Surgical correction tracheal segment resulted resection 9% total length subsequent anastomosis. Tracheoscopy performed 2 3...
The first archaeal aconitase was isolated from the cytosol of thermoacidophilic Sulfolobus acidocaldarius. Interestingly, enzyme copurified with an isocitrate lyase. This enzyme, directly converting isocitrate, reaction product reaction, also unknown in crenarchaeota, thus far. Both proteins could only be separated by SDS gel electrophoresis yielding apparent molecular masses 96 kDa for and 46 Despite its high oxygen sensitivity, enriched 27-fold to a specific activity approximately 55...