A5077987218- Mechanical Circulatory Support Devices
- Chronic Kidney Disease and Diabetes
- Heart Failure Treatment and Management
- Blood Pressure and Hypertension Studies
- Cardiac Structural Anomalies and Repair
- Transplantation: Methods and Outcomes
- Hormonal Regulation and Hypertension
- Cardiovascular Function and Risk Factors
- Diabetes Treatment and Management
- Advanced Proteomics Techniques and Applications
- Renal Diseases and Glomerulopathies
- Renin-Angiotensin System Studies
- Dialysis and Renal Disease Management
- Cardiac pacing and defibrillation studies
- Advanced Glycation End Products research
- Cardiac Arrest and Resuscitation
- Mass Spectrometry Techniques and Applications
- Sarcoidosis and Beryllium Toxicity Research
- Cardiomyopathy and Myosin Studies
- Viral Infections and Immunology Research
- Renal Transplantation Outcomes and Treatments
- Electrolyte and hormonal disorders
- Cardiovascular Effects of Exercise
- Hemodynamic Monitoring and Therapy
- Potassium and Related Disorders
Rigshospitalet
2016-2025
Copenhagen University Hospital
2015-2025
University of Copenhagen
2013-2025
Art Research Centre of the Slovak Academy of Sciences
2024
Danish National Research Foundation
2018
Steno Diabetes Centers
2003-2016
Odense University Hospital
2013
Zealand University Hospital
2013
Frederiksberg Hospital
2013
Gentofte Hospital
2013
Because of its availability, ease collection, and correlation with physiology pathology, urine is an attractive source for clinical proteomics/peptidomics. However, the lack comparable data sets from large cohorts has greatly hindered development proteomics. Here, we report establishment a reproducible, high resolution method peptidome analysis naturally occurring human urinary peptides proteins, ranging 800 to 17,000 Da, using samples 3,600 individuals analyzed by capillary electrophoresis...
Conflicting evidence of a decline in incidence microvascular complications type 1 diabetes during the last decades has been reported. To assess recent trends cumulative diabetic microangiopathy diabetes, we analyzed data from long-term prospective observational studies lasting >/=20 years.A total 600 Caucasian patients with onset between 1965 and 1984 were followed until death or year 2000. Patients divided into four groups based on onset: group A, 1965-1969 (n = 113); B, 1970-1974 130); C,...
The aim of this manuscript is to initiate a constructive discussion about the definition clinical proteomics, study requirements, pitfalls and (potential) use. Furthermore, we hope stimulate proposals for optimal use future opportunities seek unification approaches in proteomic studies. We have outlined our collective views basic principles that should be considered studies, including sample selection, choice technology appropriate quality control, need collaborative interdisciplinary...
OBJECTIVE—The objective of this study was to evaluate the safety and short-term effect adding spironolactone conventional antihypertensive treatment including diuretics maximally recommended doses an ACE inhibitor or angiotensin II receptor blocker (ARB) on albuminuria blood pressure in type 2 diabetic patients with nephropathy. RESEARCH DESIGN AND METHODS—Twenty-one nephropathy were enrolled a randomized, double-masked, cross-over study. Patients treated random order 25 mg once daily...
Urinary biomarkers for diabetes, diabetic nephropathy, and nondiabetic proteinuric renal diseases were sought. For 305 individuals, defined validated in blinded data sets using high-resolution capillary electrophoresis coupled with electrospray-ionization mass spectrometry. A panel of 40 distinguished patients diabetes from healthy individuals 89% sensitivity 91% specificity. Among 102 urinary differed significantly between normoalbuminuria a model that included 65 these correctly identified...
A limitation of proteomic methods with respect to their clinical applicability is the lack possibilities directly deduce amount a protein or peptide from particular mass spectrometry (MS) spectrum. For quantification chronic kidney disease (CKD)-specific urinary polypeptides in capillary electrophoresis coupled (CE-MS), we compared signal intensity calibration based on either creatinine stable isotope labeled synthetic marker analogues (absolute quantification) those ion counting using...
OBJECTIVE—We evaluated the renoprotective effects as reflected by short-term changes in albuminuria of dual blockade renin-angiotensin system (RAS) adding an angiotensin II receptor blocker (ARB) to treatment with maximal recommended doses ACE inhibitor (ACEI) patients type 2 diabetes and nephropathy. RESEARCH DESIGN AND METHODS—A total 20 (17 men 3 women) along hypertension nephropathy were enrolled this double-blind, randomized, two-period, crossover trial 8 weeks ARB candesartan 16 mg...
OBJECTIVE—Many patients with diabetic nephropathy (DN) have levels of albuminuria >1 g/day and blood pressure >135/85 mmHg, despite antihypertensive combination therapy, including recommended doses ACE inhibitors, e.g., lisinopril/enalapril at 20 mg daily. We tested the concept that such might benefit from dual blockade renin-angiotensin system (RAS). RESEARCH DESIGN AND METHODS—We performed a randomized double-blind crossover study 2 months treatment candesartan cilexetil 8...
Abstract Owing to its availability, ease of collection, and correlation with pathophysiology diseases, urine is an attractive source for clinical proteomics. However, many proteomic studies have had only limited impact, due factors such as modest numbers subjects, absence disease controls, small defined biomarkers, diversity analytical platforms. Therefore, it difficult merge biomarkers from different into a broadly applicable human urinary proteome database. Ideally, the methodology...
Background Urine proteome analysis is rapidly emerging as a tool for diagnosis and prognosis in disease states. For of diabetic nephropathy (DN), urinary was successfully applied pilot study. The validity the previously established proteomic biomarkers with respect to diagnostic prognostic potential assessed on separate set patients recruited at three different European centers. In this case-control study 148 Caucasian diabetes mellitus type 2 duration ≥5 years, cases DN were defined...
SUMMARY Aims Elevated urinary albumin excretion is associated with macrovascular atherosclerotic complications in Type 1 diabetes mellitus. Adhesion molecules mediate leucocyte adhesion to the endothelium early process. The present study tests hypothesis that microalbuminuria and diabetic nephropathy are elevated plasma concentrations of soluble vascular molecule (sVCAM)‐1, intercellular (sICAM)‐1, E‐selectin (sE‐selectin) aiming illustrate factors potential pathogenetic relevance for excess...
To evaluate long-term survival, development of renal end points, and decline in glomerular filtration rate (GFR) patients with type 2 diabetes diabetic nephropathy (DN) after renin-angiotensin system (RAS) inhibition multifactorial treatment cardiovascular risk factors have become standard care.All DN (n = 543) at the Steno Diabetes Center were followed during 2000-2010. GFR was measured yearly 51Cr-EDTA plasma clearance. Annual determined least three measurements over a minimum 3 years...
Abstract Aims Heart failure (HF) is a major public health concern worldwide. The diversity of HF makes it challenging to decipher the underlying complex pathological processes using single biomarkers. We examined association between urinary peptides and with reduced (HFrEF), mid‐range (HFmrEF) preserved (HFpEF) ejection fraction, defined based on European Society Cardiology guidelines, links these peptide biomarkers molecular pathophysiology. Methods results Analysable data from 5608...
Arrhythmogenic right ventricular cardiomyopathy (ARVC) is characterized by high propensity to life-threatening arrhythmias and progressive loss of heart muscle. More than 40% reported genetic variants linked ARVC reside in the
Recently, a dramatic decline in the cumulative incidence of diabetic nephropathy (<10% after 25 years diabetes) has been reported insulin-dependent diabetes mellitus (IDDM) patients diagnosed before age 15 between 1961 and 1980. In clinic-based study, we assessed recent trends nephropathy. All 356 whom IDDM was 41 1965 1979, identified 1984, were followed until 1991 or death. Caucasians resided Copenhagen. The incidences (life-table method) (urinary albumin excretion ≥300 mg/24 h two...
OBJECTIVE—The purpose of this study was to assess agreement between glomerular filtration rate (GFR) and the decline in GFR estimated with Modification Diet Renal Disease (MDRD) Study Group equation or Cockcroft-Gault formula measured by plasma clearance 51Cr-EDTA. RESEARCH DESIGN AND METHODS—We followed a cohort 156 microalbuminuric type 2 diabetic patients for 8 years four measurements another 227 overt nephropathy 6.5 (range 3–17) seven (3–22) GFR. RESULTS—For microalbuminuria, mean ± SD...