A5018391034- Advanced Proteomics Techniques and Applications
- Renal Diseases and Glomerulopathies
- Complement system in diseases
- Chronic Kidney Disease and Diabetes
- GDF15 and Related Biomarkers
- Advanced Glycation End Products research
- Blood groups and transfusion
- Renal Transplantation Outcomes and Treatments
- Extracellular vesicles in disease
- Cardiac Fibrosis and Remodeling
- Protease and Inhibitor Mechanisms
- Retinal Diseases and Treatments
- Protein Hydrolysis and Bioactive Peptides
- Peptidase Inhibition and Analysis
- Diabetes and associated disorders
- Transplantation: Methods and Outcomes
- COVID-19 Clinical Research Studies
- Renin-Angiotensin System Studies
- S100 Proteins and Annexins
- Cancer Research and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Single-cell and spatial transcriptomics
- Health, Environment, Cognitive Aging
- Cancer Mechanisms and Therapy
- Biomarkers in Disease Mechanisms
Mosaiques Diagnostics and Therapeutics (Germany)
2019-2023
Universitätsklinikum Aachen
2021
RWTH Aachen University
2019-2021
Gentofte Hospital
2021
Yueyang Hospital
2014
Shanghai University of Traditional Chinese Medicine
2014
Heidelberg University
2010
Abstract Aims Heart failure (HF) is a major public health concern worldwide. The diversity of HF makes it challenging to decipher the underlying complex pathological processes using single biomarkers. We examined association between urinary peptides and with reduced (HFrEF), mid‐range (HFmrEF) preserved (HFpEF) ejection fraction, defined based on European Society Cardiology guidelines, links these peptide biomarkers molecular pathophysiology. Methods results Analysable data from 5608...
Identification of significant changes in urinary peptides may enable improved understanding molecular disease mechanisms. We aimed towards identifying associated with critical course COVID-19 to yield hypotheses on pathophysiological mechanisms development. In this multicentre prospective study urine samples PCR-confirmed patients were collected different centres across Europe. The peptidome 53 at WHO stages 6-8 and 66 1-3 was analysed using capillary electrophoresis coupled mass...
ABSTRACT Background and hypothesis Specific urinary peptides hold information on disease pathophysiology, which, in combination with artificial intelligence, could enable non-invasive assessment of chronic kidney (CKD) aetiology. Existing approaches are generally specific for the diagnosis single aetiologies. We present development models able to simultaneously distinguish spatially visualize multiple CKD Methods The peptide data 1850 healthy control (HC) [diabetic (DKD), immunoglobulin A...
The peptidomes of spent hemodialysate, urine, and plasma are investigated, to shed light on peptide handling in the kidney.
Abstract Aims Cardiac allograft vasculopathy (CAV) is the major long‐term complication after heart transplantation, leading to mortality and re‐transplantation. As available non‐invasive biomarkers are scarce for CAV screening, we aimed identify a proteomic signature CAV. Methods results We measured urinary proteome by capillary electrophoresis coupled with mass spectrometry in 217 transplantation recipients (mean age: 55.0 ± 14.4 years; women: 23.5%), including 76 (35.0%) patients diagnosed...
Nephropathy related to obesity lacks a pathophysiological understanding and definite diagnostic pathways by biomarkers.In this study we investigated the association between urinary peptides body mass index (BMI) renal function in proteome data sets from 4015 individuals.A total of 365 were identified be significantly associated with BMI. The majority these collagen fragments. In addition, most also demonstrated significant concordant estimated glomerular filtration rate (eGFR) cohort,...
Defective complement activation has been associated with various types of kidney disease. This led to the hypothesis that specific urine fragments may be disease etiologies, and progression reflected by changes in these fragments. We investigated occurrence urine, their association function etiology 16,027 subjects, using mass spectrometry based peptidomics data from Human Urinary Proteome/Peptidome Database. Twenty-three different urinary peptides originating proteins C3, C4 factor B (CFB)...
Abstract The polymeric immunoglobulin receptor (pIgR) transports immunoglobulins from the basolateral to apical surface of epithelial cells. PIgR was recently shown be associated with kidney dysfunction. immune defense is initiated at cells where N-terminal domain pIgR, termed secretory component (SC), proteolytically cleaved and released either unbound (free SC) or bound immunoglobulins. aim our study evaluate association pIgR peptides cardio-renal syndrome in a large cohort obtain...
Given the association of diabetic retinopathy (DR) and kidney disease, we investigated urinary peptidome to presence deterioration DR in a post hoc analysis trials investigating effect candesartan on progression type 1 2 diabetes, respectively.Baseline peptidomic was performed random selection 783 792 subjects two randomized controlled trials, DIRECT-Protect 2, respectively. End points were two-step (RET2) three-step (RET3) change Early Treatment Diabetic Retinopathy Study protocol (ETDRS)...
Abstract The cardiorenal syndrome (CRS) is defined as the confluence of heart-kidney dysfunction. This study investigates molecular differences at level urinary peptidome between CRS patients and controls their association to disease pathophysiology. (n = 353) was matched for age sex with 356) a 1:1 ratio. Changes in versus were identified after applying Mann–Whitney test, followed by correction multiple testing. Proteasix tool applied investigate predicted proteases involved CRS-associated...
Defective complement activation has been associated with various types of kidney disease. This led to the hypothesis that specific urine fragments may be disease etiologies, and progression reflected by changes in these fragments. We investigated occurrence urine, their association function, proteinuria etiology. Mass spectrometry based peptidomics data from Human Urinary Proteome/Peptidome Database were extracted distribution peptides different etiologies controls was investigated. All...
Given the pathophysiological continuum of chronic kidney disease (CKD), different molecular determinants affecting progression may be associated with distinct phases; thus, identification these players are crucial for guiding therapeutic decisions, ideally in a non-invasive, repeatable setting. Analyzing urinary peptidome has been proven an efficient method biomarker determination CKD, among other diseases. In this work, after applying several selection criteria, urine samples from 317 early...
Abstract Background The impact of artificial intelligence combined with advanced techniques is ever-increasing in the biomedical field appearing promising, among others, chronic kidney disease (CKD) diagnosis. However, existing models are often single-aetiology specific. Proposed here a pipeline for development single able to distinguish and spatially visualize multiple CKD aetiologies. Methods Acquired were from Human Urinary Proteome Database urinary peptide data 1850 healthy control (HC)...
Objective To investigate the safety and efficiency of three different double-channel chemotherapy regimens in treatment advanced colorectal cancer.Methods 60 cases cancer with definite pathological diagnosis were randomly divided into groups 20 per group:the CPT-11+DDP group,the raltitrexed+DDP group fluorouracil+DDP group.Every 21 days was one course treatment,all patients received at least 2 cycles treatment.The recent effect,PFS,mOS,toxicity quality life observed respectively.Results 40...
Introduction: Thrombomodulin (TM) is predominantly a vascular endothelial cell plasma membrane glycoprotein that, via distinct structural domains, interacts with multiple ligands, thereby modulating coagulation, fibrinolysis, complement activation, inflammation and proliferation. We have previously shown that TM protects against diabetic nephropathy (DN) through activation of protein C(PC), which inhibits glomerular apoptosis. Recent studies showed mediates cytoprotective effects independent...
Abstract BACKGROUND AND AIMS Currently >20 000 native peptides in urine are known that highly dynamic and able to display the status of different organs, especially kidney. The characterization urinary peptide profiles (UPP) enables depiction kidney disease severity, progression, fibrosis, informs about etiology. Advanced machine learning algorithms enable combining changes very complex UPP associated with specific etiologies reducing dataspace only few dimensions. Here, we show...
Abstract Background and Aims The cardiorenal syndrome (CRS) reflects the complex interplay between kidney heart diseases, but its molecular basis remains poorly understood. Multiple studies have demonstrated association of urinary biomarkers with both diseases. However, their relevance involvement in CRS not been investigated yet. To address this gap, a study was designed aim to compare specific for failure (HF) chronic disease (CKD) peptides representing CRS, ultimate target connect these...
Abstract Background and Aims The polymeric immunoglobulin receptor (pIgR) which transports immunoglobulins from the basolateral to apical surface of epithelial cells was recently shown be associated with kidney dysfunction. immune defense is initiated at where N-terminal domain pIgR, termed secretory component (SC), proteolytically cleaved released either unbound (free SC) or bound immunoglobulins. aim our study show association pIgR peptides cardio-renal syndrome in a large cohort get...
Abstract Background and Aims Changes in complement factors have been associated with chronic kidney disease, most prominently the context of C3 glomerulopathy. Based on these reports, hypothesis was generated that specific urine fragments are disease etiologies, progression may be reflected by changes proteins or peptides. In this study, we aimed at investigating occurrence urine, their association function general etiology particular. Methods Urine samples from patients different etiologies...
Abstract Background and Aims The physiological related body fluids, urine, plasma hemodialysis (HD) fluid spent dialysate (a mixture of technical HD patient´s ultrafiltrate) are rich biological sources information especially in the context kidney diseases. Of particular relevance proteins peptides, as they reflect essentially every process. Urine is produced a result glomerular filtration subsequent tubular reabsorption kidney. In analogy, ultrafiltration by an artificial (dialysis...
Abstract Background and Aims Thymosin β4 (Tβ4) is an abundant peptide in urine plasma, involved multiple processes including cell morphology, wound healing, inflammation. A major, yet not well defined role of Tβ4 was described the context kidney cardiovascular disease. Knock out resulted, among others, increased albuminuria a nephrotoxic nephritis model. In animal models it has been shown that can modify renal disease, fibrosis However, most data are derived from relevance human less clear....