A5002995524- thermodynamics and calorimetric analyses
- Plant Ecology and Soil Science
- Plant biochemistry and biosynthesis
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Cardiovascular Effects of Exercise
- Cardiomyopathy and Myosin Studies
- Cardiac Ischemia and Reperfusion
- Cardiac Fibrosis and Remodeling
- Adipose Tissue and Metabolism
- Receptor Mechanisms and Signaling
- Mitochondrial Function and Pathology
- Machine Learning in Bioinformatics
- Cardiovascular Function and Risk Factors
- Peptidase Inhibition and Analysis
- Sports injuries and prevention
- Cardiac Arrest and Resuscitation
- Advanced Chemical Sensor Technologies
- Nitric Oxide and Endothelin Effects
- Chromosomal and Genetic Variations
- ATP Synthase and ATPases Research
- Redox biology and oxidative stress
- Heart Failure Treatment and Management
Spanish National Centre for Cardiovascular Research
2016-2024
University of Copenhagen
2020-2022
The mobilization of transposable elements (TEs) is suppressed by host genome defense mechanisms. Recent studies showed that the cis-regulatory region Arabidopsis thaliana COPIA78/ONSEN retrotransposons contains heat-responsive (HREs), which cause their activation during heat stress. However, it remains unknown whether this a common and potentially conserved trait how has evolved. We show ONSEN, COPIA37, TERESTRA, ROMANIAT5 are major families TEs in A. lyrata thaliana. Heat-responsiveness...
Exercise training, and catecholaminergic stimulation, increase the incidence of arrhythmic events in patients affected with arrhythmogenic right ventricular cardiomyopathy correlated plakophilin-2 (PKP2) mutations. Separate data show that reduced abundance PKP2 leads to dysregulation intracellular Ca
Arrhythmogenic right ventricular cardiomyopathy (ARVC) is characterized by high propensity to life-threatening arrhythmias and progressive loss of heart muscle. More than 40% reported genetic variants linked ARVC reside in the
Mass spectrometry-based proteomics has had a formidable development in recent years, increasing the amount of data handled and complexity statistical resources needed. Here we present SanXoT, an open-source, standalone software package for analysis high-throughput, quantitative experiments. SanXoT is based on our previously developed weighted spectrum, peptide protein model been specifically designed to be modular, scalable user-configurable. allows limitless workflows that adapt most...
Post-translational modifications hugely increase the functional diversity of proteomes. Recent algorithms based on ultratolerant database searching are forging a path to unbiased analysis peptide by shotgun mass spectrometry. However, these approaches identify only one-half modified forms potentially detectable and do not map residue. Moreover, tools for quantitative currently lacking. Here, we present suite that allows comprehensive identification modifications, pinpoints residues, enables...
Abstract Post translational modifications (PTMs) are covalent of proteins that can range from small chemical to addition entire proteins. PTMs contribute regulation protein function and thereby greatly increase the functional diversity proteome. In heart, a few well-studied PTMs, such as phosphorylation glycosylation, known play essential roles for cardiac function. Yet, only fraction ~ 300 have been studied in context. Here we investigated proteome-wide map present human hearts by utilizing...
In most eukaryotic cells, the mitochondrial DNA (mtDNA) is transmitted uniparentally and present in multiple copies derived from clonal expansion of maternally inherited mtDNA. All are therefore near-identical, or homoplasmic. The presence >1 mtDNA variant same cytoplasm can arise naturally result new medical technologies aimed at preventing genetic diseases improving fertility. latter called divergent nonpathologic heteroplasmy (DNPH). We hypothesized that DNPH maladaptive usually prevented...
Abstract Reperfusion alters post-myocardial infarction (MI) healing; however, very few systematic studies report the early molecular changes following ischemia/reperfusion (I/R). Alterations in remote myocardium have also been neglected, disregarding its contribution to post-MI heart failure (HF) development. This study characterizes protein dynamics and contractile abnormalities ischemic during one week after MI. Closed-chest 40 min I/R was performed 20 pigs sacrificed at 120 min, 24 hours,...
Changes in the oxidation state of protein Cys residues are involved cell signalling and play a key role variety pathophysiological states. We had previously developed GELSILOX, an in-gel method that enables large-scale, parallel analysis dynamic alterations to redox sites abundance changes. Here we present FASILOX, further development GELSILOX approach featuring: i) significantly increased peptide recovery, ii) enhanced sensitivity for detection oxidative alterations, iii) streamlined...
While reperfusion, or restoration of coronary blood flow in acute myocardial infarction, is a requisite for salvage, it can paradoxically induce specific damage known as ischemia/reperfusion (I/R) injury. Our understanding the precise pathophysiological molecular alterations leading to I/R remains limited. In this study, we conducted comprehensive and unbiased time-course analysis post-translational modifications (PTMs) post-reperfused myocardium two different animal models (pig mouse)...
Our heart is comprised of many different cell types that all contribute to cardiac function. An important step in deciphering the molecular complexity our decipher composition various types. Here we set out delineate a comprehensive protein expression profile two most prevalent heart: cardiomyocytes and fibroblasts. To this end, isolated fibroblasts from rat hearts combined state-of-the-art flow cytometry with high-resolution mass spectrometry investigate their proteome profiles right after...
Abstract Heart failure is a multifactorial disease that affects an estimated 38 million people worldwide. Current pharmacotherapy of heart with reduced ejection fraction (HFrEF) includes combination therapy angiotensin-converting enzyme inhibitors (ACEi) and β-adrenergic receptor blockers (β-AR blockers), also used as treatment for non-cardiac conditions. Our knowledge the molecular changes accompanying ACEi β-AR limited. Here, we applied proteomics phosphoproteomics approaches to profile...
SUMMARY Post-translational modifications hugely increase the functional diversity of proteomes. Recent algorithms based on ultratolerant database searching are forging a path to unbiased analysis peptide by shotgun mass spectrometry. However, these approaches identify only half modified forms potentially detectable and do not map residue. Moreover, tools for quantitative currently lacking. Here, we present suite that allow comprehensive identification modifications, pinpoint residues, enable...