A5055175015- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Genetic and Mutation Studies
- Photosynthetic Processes and Mechanisms
- Plant Ecology and Soil Science
- thermodynamics and calorimetric analyses
- Plant biochemistry and biosynthesis
- Plant Disease Resistance and Genetics
- DNA Repair Mechanisms
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Genomics and Chromatin Dynamics
- Genetic Mapping and Diversity in Plants and Animals
- Plant Virus Research Studies
- Wheat and Barley Genetics and Pathology
- Soybean genetics and cultivation
- Bioenergy crop production and management
- Legume Nitrogen Fixing Symbiosis
- Cell Image Analysis Techniques
- Plant and Biological Electrophysiology Studies
- Plant Gene Expression Analysis
- Plant Parasitism and Resistance
- Light effects on plants
Czech Academy of Sciences, Institute of Experimental Botany
2017-2024
Palacký University Olomouc
2021-2024
Australian Centre for Plant Functional Genomics
2024
Max Planck Institute for Plant Breeding Research
2012-2021
Leibniz Institute of Plant Genetics and Crop Plant Research
2004-2016
Gregor Mendel Institute of Molecular Plant Biology
2006-2014
Austrian Academy of Sciences
2008-2014
Leibniz Association
2003-2006
We have used an approximately 8.7-Mb BAC contig of Arabidopsis thaliana Chromosome 4 to trace homeologous chromosome regions in 21 species the family Brassicaceae. Homeologs this segment could be identified all tested species. Painting pachytene chromosomes Calepina, Conringia, and Sisymbrium (2n = 14, 16), traditionally placed tribe Brassiceae, showed one copy contig, while remaining taxa 14-30) revealed three, three Brassica 34, 36, 38) Erucastrum gallicum 30) had six copies corresponding...
Abstract Epigenetic factors determine responses to internal and external stimuli in eukaryotic organisms. Whether how environmental conditions feed back the epigenetic landscape is more a matter of suggestion than substantiation. Plants are suitable organisms with which address this question due their sessile lifestyle diversification regulators. We show that several repetitive elements Arabidopsis thaliana under regulation by transcriptional gene silencing at ambient temperatures upon short...
Evolution of chromosome complements can be resolved by genome sequencing, comparative genetic mapping, and painting. Previously, comparison maps gene-based phylogenies suggested that the karyotypes Arabidopsis thaliana (n = 5) related species with six or seven pairs were derived from an ancestral karyotype eight pairs. To test this hypothesis, we applied multicolor painting using contiguous bacterial artificial pools A. arranged according to lyrata Capsella rubella (both n 8) thaliana,...
Adverse conditions can trigger DNA damage as well repair responses in plants. A variety of stress factors are known to stimulate homologous recombination, the most accurate pathway, by increasing concentration necessary enzymatic components and frequency events. This effect has been reported last into subsequent generations not exposed stress. To establish a basis for genetic analysis this transgenerational memory, broad range treatments was tested quantitative effects on recombination...
The C-value paradox remains incompletely resolved after >40 yr and is exemplified by 2,350-fold variation in genome sizes of flowering plants. carnivorous Lentibulariaceae genus Genlisea, displaying a 25-fold range sizes, promising subject to study mechanisms consequences evolutionary size variation. Applying genomic, phylogenetic, cytogenetic approaches, we uncovered bidirectional evolution within the Genlisea. Genlisea nigrocaulis Steyerm. (86 Mbp) has probably shrunk retroelement...
Abstract Background Polyploidization is the multiplication of whole chromosome complement and has occurred frequently in vascular plants. Maintenance stable polyploid state over generations requires special mechanisms to control pairing distribution more than two homologous chromosomes during meiosis. Since a minimal number crossover events essential for correct segregation, we investigated whether polyploidy an influence on frequency meiotic recombination. Results Using genetically linked...
The mobilization of transposable elements (TEs) is suppressed by host genome defense mechanisms. Recent studies showed that the cis-regulatory region Arabidopsis thaliana COPIA78/ONSEN retrotransposons contains heat-responsive (HREs), which cause their activation during heat stress. However, it remains unknown whether this a common and potentially conserved trait how has evolved. We show ONSEN, COPIA37, TERESTRA, ROMANIAT5 are major families TEs in A. lyrata thaliana. Heat-responsiveness...
Abstract Cereal grains are an important source of food and feed. To provide comprehensive spatiotemporal information about biological processes in developing seeds cultivated barley (Hordeum vulgare L. subsp. vulgare), we performed a transcriptomic study the embryo, endosperm, seed maternal tissues collected from 4–32 days after pollination. Weighted gene co-expression network motif enrichment analyses identified specific groups genes transcription factors (TFs) potentially regulating tissue...
DNA replication in cycling eukaryotic cells necessitates the reestablishment of chromatin after nucleosome redistribution from parental to two daughter strands. Chromatin assembly factor 1 (CAF-1), a heterotrimeric complex consisting three subunits (p150/p60/p48), is one replication-coupled factors involved reconstitution S-phase chromatin. CAF-1 required vitro for onto newly replicated human and Arabidopsis thaliana, defects yeast (Saccharomyces cerevisiae) affect damage repair processes,...
Covalent modification by methylation of cytosine residues represents an important epigenetic hallmark. While sequence analysis after bisulphite conversion allows correlative analyses with single-base resolution, functional interference DNA is less precise, due to the complexity enzymes and their targets. A cytidine analogue, 5-azacytidine, frequently used as inhibitor methyltransferases, but its rapid degradation in aqueous solution problematic for culture periods longer than a few hours....
Gene model annotations are important community resources that ensure comparability and reproducibility of analyses typically the first step for functional annotation genomic regions. Without up-to-date genome annotations, sequences cannot be used to maximum advantage. It is therefore essential regularly update gene by integrating latest information guarantee reference can remain a common basis various types analyses. Here, we report an improvement Arabidopsis lyrata using extensive RNA-seq...
The complex nature of crop genomes has long prohibited the efficient isolation agronomically relevant genes. However, recent advances in next-generation sequencing technologies provide new ways to accelerate fine-mapping and gene crops. We used RNA allelic six-rowed spike3 (vrs3) mutants with altered spikelet development for identification functional analysis barley (Hordeum vulgare). Variant calling two vrs3 revealed that VRS3 encodes a putative histone Lys demethylase conserved zinc finger...
Abstract The endosperm is a seed tissue unique to flowering plants. Due its central role in nourishing and protecting the embryo, development subject parental conflicts adaptive processes, which led evolution of parent-of-origin–dependent gene regulation. higher-order chromatin organization regulating genome was long ignored due technical hindrance. We developed combination approaches analyze nuclear structure Arabidopsis thaliana endosperm. Endosperm nuclei showed less condensed than other...
Abstract Epigenetic changes of gene expression can potentially be reversed by developmental programs, genetic manipulation, or pharmacological interference. However, a case transcriptional silencing, originally observed in tetraploid Arabidopsis thaliana plants, created an epiallele resistant to many mutations inhibitor treatments that activate other suppressed genes. This raised the question about molecular basis this extreme stability. A combination forward and reverse genetics drug...
Abstract Ground levels of solar UV-B radiation induce DNA damage. Sessile phototrophic organisms such as vascular plants are recurrently exposed to sunlight and require photoreception, flavonols shielding, direct reversal pyrimidine dimers nucleotide excision repair for resistance against radiation. However, the frequency UV-B-induced mutations is unknown in plants. Here we quantify amount types offspring Arabidopsis thaliana wild-type UV-B-hypersensitive mutants simulated natural over their...
DNA damage repair is an essential cellular mechanism that maintains genome stability. Here, we show the nonmethylable cytidine analog zebularine induces a response in Arabidopsis thaliana, independent of changes methylation. In contrast to genotoxic agents induce cell cycle stage-independent manner, specifically during strand synthesis replication. The signaling this mediated by additive activity ATAXIA TELANGIECTASIA MUTATED AND RAD3-RELATED and kinases, which cause postreplicative arrest...