A5000815036- Cardiac Imaging and Diagnostics
- Cardiac Ischemia and Reperfusion
- Advanced MRI Techniques and Applications
- Cardiac electrophysiology and arrhythmias
- Chemotherapy-induced cardiotoxicity and mitigation
- Cardiovascular Function and Risk Factors
- Cardiac Arrest and Resuscitation
- Cardiac Fibrosis and Remodeling
- Cardiac Arrhythmias and Treatments
- Cardiovascular Effects of Exercise
- Acute Myocardial Infarction Research
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Cardiac Valve Diseases and Treatments
- Metabolism, Diabetes, and Cancer
- Pulmonary Hypertension Research and Treatments
- Cancer-related cognitive impairment studies
- Cardiomyopathy and Myosin Studies
- Polymer Nanocomposite Synthesis and Irradiation
- Nitric Oxide and Endothelin Effects
- Lanthanide and Transition Metal Complexes
- Electrospun Nanofibers in Biomedical Applications
- Coronary Interventions and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Diabetes Treatment and Management
Spanish National Centre for Cardiovascular Research
2015-2025
Centro de Investigación Biomédica en Red
2017-2025
Centro de Investigación en Red en Enfermedades Cardiovasculares
2017-2025
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2024
Hospital Universitario Fundación Jiménez Díaz
2024
Boston Children's Hospital
2019
Hospital Universitari i Politècnic La Fe
2017
Philips (Spain)
2017
Complejo Hospitalario de Salamanca
2017
Icahn School of Medicine at Mount Sinai
2017
Anthracycline-induced cardiotoxicity is a major clinical problem, and early markers are needed. The purpose of this study was to identify doxorubicin-induced by serial multiparametric cardiac magnetic resonance (CMR) its pathological correlates in large animal model. Twenty pigs were included. Of these, 5 received biweekly intracoronary doxorubicin doses (0.45 mg/kg/injection) followed until sacrifice at 16 weeks. Another 3 A third group sacrificed after the dose. All groups underwent weekly...
Post-ischemia/reperfusion (I/R) myocardial edema was recently shown to follow a consistent bimodal pattern: an initial wave of appears on reperfusion and dissipates at 24 h, followed by deferred that initiates days after infarction, peaking 1 week.This study examined the pathophysiology underlying this post-I/R edematous reaction.Forty instrumented pigs were assigned different infarction protocols. Edematous reaction evaluated water content quantification, serial cardiac magnetic resonance...
Clinical protocols aimed to characterize the post-myocardial infarction (MI) heart by cardiac magnetic resonance (CMR) need be standardized take account of dynamic biological phenomena evolving early after index ischemic event. Here, we evaluated time course edema reaction in patients with ST-segment-elevation MI CMR and assessed its implications for myocardium-at-risk (MaR) quantification both a large-animal model.A total 16 anterior successfully treated primary angioplasty matched controls...
Pre-reperfusion administration of intravenous (IV) metoprolol reduces infarct size in ST-segment elevation myocardial infarction (STEMI). This study sought to determine how this cardioprotective effect is influenced by the timing therapy having either a long or short bolus-to-reperfusion interval. We performed post hoc analysis METOCARD-CNIC (effect METOprolol CARDioproteCtioN during an acute InfarCtion) trial, which randomized anterior STEMI patients IV control before mechanical...
Severe coronavirus disease-2019 (COVID-19) can progress to an acute respiratory distress syndrome (ARDS), which involves alveolar infiltration by activated neutrophils. The beta-blocker metoprolol has been shown ameliorate exacerbated inflammation in the myocardial infarction setting. purpose of this study was evaluate effects on and function patients with COVID-19–associated ARDS. A total 20 COVID-19 ARDS invasive mechanical ventilation were randomized (15 mg daily for 3 days) or control...
Anthracycline-induced cardiotoxicity (AIC) debilitates quality of life in cancer survivors. Serial characterizations are lacking the molecular processes occurring with AIC. The aim this study was to characterize AIC progression a mouse model from early (subclinical) advanced heart failure stages, an emphasis on cardiac metabolism and mitochondrial structure function. CD1 mice received 5 weekly intraperitoneal doxorubicin injections (5 mg/kg) were followed by serial echocardiography for 15...
Background: Several T2-mapping sequences have been recently proposed to quantify myocardial edema by providing T2 relaxation time values.However, no sequence has ever validated against actual water content for detection.In addition, these are either timeconsuming or require specialized software data acquisition and/or post-processing, factors impeding their routine clinical use.Our objective was obtain in vivo validation of a fast and accurate (T2 gradient-spin-echo [GraSE]) that can be...
The impact of cardioprotective strategies and ischemia duration on postischemia/reperfusion (I/R) myocardial tissue composition (edema, myocardium at risk, infarct size, salvage, intramyocardial hemorrhage, microvascular obstruction) is not well understood.To study the effect protective interventions temporal dynamics in a translational animal model I/R by use state-of-the-art imaging technology.Four 5-pig groups underwent different protocols: 40-minute (prolonged ischemia, controls),...
Abstract Aims Anthracycline-induced cardiotoxicity (AIC) is a serious adverse effect among cancer patients. A central mechanism of AIC irreversible mitochondrial damage. Despite major efforts, there are currently no effective therapies able to prevent AIC. Methods and results Forty Large-White pigs were included. In Study 1, 20 randomized 1:1 remote ischaemic preconditioning (RIPC, 3 cycles 5 min leg ischaemia followed by reperfusion) or pretreatment. RIPC was performed immediately before...
Abstract Aims The aim of this study was to changes in coronary microcirculation status during and after several cycles anthracycline treatment. Methods results Large-white male pigs (n=40) were included different experimental protocols (ExPr.) according cumulative exposure [0.45 mg/kg intracoronary (IC) doxorubicin per injection] follow-up: control (no doxorubicin); single injection sacrifice either at 48 h (ExPr. 1) or 2 weeks 2); 3 injections apart (low dose) 3) 12 4) third injection; five...
Hutchinson-Gilford progeria syndrome (HGPS) is an extremely rare genetic disorder for which no cure exists. The disease characterized by premature aging and inevitable death in adolescence due to cardiovascular complications. Most HGPS patients carry a heterozygous de novo LMNA c.1824C > T mutation, provokes the expression of dominant-negative mutant protein called progerin. Therapies proven effective HGPS-like mouse models have yielded only modest benefit clinical trials. To overcome gap...
Background Autologous adipose tissue–derived mesenchymal stem cells ( ATMSC s) therapy is a promising strategy to improve post–myocardial infarction outcomes. In porcine model of acute myocardial infarction, we studied the long‐term effects and mechanisms involved in allogeneic s administration on performance. Methods Results Thirty‐eight pigs underwent 50 minutes coronary occlusion; study was completed 33 pigs. After reperfusion, or culture medium (vehicle) were intracoronarily...
Background Transplantation of adventitial pericytes ( APC s) promotes cardiac repair in murine models myocardial infarction. The aim present study was to confirm the benefit therapy a large animal model. Methods and Results We performed blind, randomized, placebo‐controlled trial swine model reperfused A first used human s hAPC from patients undergoing coronary artery bypass graft surgery. second allogeneic sAPC s). Primary end points were (1) ejection fraction as assessed by magnetic...
Cardiac optical mapping is the gold standard for measuring complex electrophysiology in ex vivo heart preparations. However, new methods have been elusive. We aimed at developing and validating an experimental method performing cardiac pig models.First, we characterized excitation-ratiometric properties during pacing ventricular fibrillation (VF) of two near-infrared voltage-sensitive dyes (di-4-ANBDQBS/di-4-ANEQ(F)PTEA) optimized imaging blood-perfused tissue (n = 7). Then, optical-fibre...
Abstract Early metoprolol administration protects against myocardial ischemia–reperfusion injury, but its effect on infarct size progression (ischemic injury) is unknown. Eight groups of pigs (total n = 122) underwent coronary artery occlusion varying duration (20, 25, 30, 35, 40, 45, 50, or 60 min) followed by reperfusion. In each group, were randomized to i.v. (0.75 mg/kg) vehicle (saline) 20 min after ischemia onset. The primary outcome measure was (IS) day7 cardiac magnetic resonance...
Myocardial perfusion cardiovascular magnetic resonance (pCMR) using first-pass contrast-enhanced imaging could play an important role in the detection of epicardial and microvascular coronary artery disease. Recently, emergence quantitative pCMR has provided a more reliable observer-independent analysis compared to visual interpretation dynamic images. This review aims cover basics pCMR, from acquisition protocols, its use preclinical clinical studies, image reconstruction motion handling,...
Abstract Reperfusion alters post-myocardial infarction (MI) healing; however, very few systematic studies report the early molecular changes following ischemia/reperfusion (I/R). Alterations in remote myocardium have also been neglected, disregarding its contribution to post-MI heart failure (HF) development. This study characterizes protein dynamics and contractile abnormalities ischemic during one week after MI. Closed-chest 40 min I/R was performed 20 pigs sacrificed at 120 min, 24 hours,...
Abstract Right ventricular (RV) failure remains the strongest determinant of survival in pulmonary hypertension (PH). We aimed to identify relevant mechanisms, beyond pressure overload, associated with maladaptive RV hypertrophy PH. To separate effect overload from other potential we developed pigs two experimental models PH (M1, by vein banding and M2, aorto-pulmonary shunting) compared them a model pure (M3, artery banding) sham-operated group. Animals were assessed at 1 8 months right...
Left atrial (LA) remodeling after an acute myocardial infarction (MI) is poorly characterized regarding its determinants or effect on ischemic mitral regurgitation (MR) development. The purpose of this study was: 1) to compare LA structural in experimental MI swine models recapitulating the effects left ventricular (LV) dysfunction, MR, and (LAI); 2) analyze how influences MR Three were generated: proximal circumflex (LCx) coronary artery occlusion involving branch (LAI group); LCx not (LCx...