A5017532824- Cardiac Arrest and Resuscitation
- Pneumonia and Respiratory Infections
- Extracellular vesicles in disease
- Nosocomial Infections in ICU
- Cardiac Ischemia and Reperfusion
- Traumatic Brain Injury and Neurovascular Disturbances
- Respiratory Support and Mechanisms
- Heart Rate Variability and Autonomic Control
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Acute Ischemic Stroke Management
- Electrospun Nanofibers in Biomedical Applications
- Airway Management and Intubation Techniques
- Thermal Regulation in Medicine
- Tissue Engineering and Regenerative Medicine
- Non-Invasive Vital Sign Monitoring
- Cerebrovascular and Carotid Artery Diseases
- Cardiovascular Disease and Adiposity
- S100 Proteins and Annexins
- Cardiovascular Issues in Pregnancy
- Sepsis Diagnosis and Treatment
- Antioxidant Activity and Oxidative Stress
- Cardiac Structural Anomalies and Repair
- Cardiac Fibrosis and Remodeling
- Intensive Care Unit Cognitive Disorders
Universitat de Barcelona
2017-2025
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2017-2025
Erasmus MC
2021-2025
Hospital Clínic de Barcelona
2016-2025
Erasmus University Rotterdam
2022-2023
Erasmus MC Cancer Institute
2023
Fundacao Joaquim Nabuco
2019
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2019
Spanish National Centre for Cardiovascular Research
2016
Background Autologous adipose tissue–derived mesenchymal stem cells ( ATMSC s) therapy is a promising strategy to improve post–myocardial infarction outcomes. In porcine model of acute myocardial infarction, we studied the long‐term effects and mechanisms involved in allogeneic s administration on performance. Methods Results Thirty‐eight pigs underwent 50 minutes coronary occlusion; study was completed 33 pigs. After reperfusion, or culture medium (vehicle) were intracoronarily...
Abstract Background Streptococcus pneumoniae , a primary cause of community-acquired pneumonia (CAP), is typically treated with β-lactams and macrolides or quinolones. Corticosteroids are now recommended as adjunctive therapy in severe CAP to improve outcomes. I n this prospective randomized animal study, we evaluated the bactericidal efficacy various antibiotic regimens combined corticosteroids using porcine pneumococcal model. Results In 30 White-Landrace female pigs, was induced by...
Circulating extracellular vesicles (EVs) have a great impact on human health as biomarkers and messengers in intercellular signalling. We aimed to determine how the miRNA profile of circulating EVs during an acute coronary event interferes with vasculogenic potential endothelial cells (EC). were purified from plasma patients phase non-ST segment elevation myocardial infarction (NSTEMI, n = 33) healthy donors (n 19) used control group. Human ECs treated EV suspension (5 × 107 particles/cm2)...
Background Endovascular catheters and devices used for thrombectomy in patients who had a stroke can damage the vessel lumen leading to microthrombi. During recanalisation, microthrombi could migrate distally occlude cerebral microvasculature, potentially limiting benefit of recanalisation therapy. Objectives To describe vascular injury occurring after endovascular therapy (EVT), with stent retrievers (SR) direct aspiration (DA), open up avenues further improvement EVT technique. Methods SR...
Pseudomonas aeruginosa pneumonia is commonly treated with systemic antibiotics to ensure adequate treatment of multidrug resistant (MDR) bacteria. However, intravenous (IV) often achieve suboptimal pulmonary concentrations. We therefore aimed evaluate the effect inhaled amikacin (AMK) plus IV meropenem (MEM) on bactericidal efficacy in a swine model monolateral MDR P. pneumonia.We ventilated 18 pigs for up 102 h. At 24 h after bacterial challenge, animals were randomized receive 72 either...
<h3>BACKGROUND:</h3> Mechanical insufflation-exsufflation (MI-E) has been proposed as a potential strategy to generate high expiratory flows and simulate cough in the critically ill. However, efficacy safety of MI-E during invasive mechanical ventilation are still be fully elucidated. This study intubated mechanically ventilated pigs aimed evaluate effects 8 combinations pressures on mucus displacement, respiratory flows, well mechanics hemodynamics. <h3>METHODS:</h3> Six healthy...
Futile recanalization hampers prognoses of ischemic stroke after successful mechanical thrombectomy, hypothetically through post-recanalization perfusion deficits, onset-to-groin delays and sex effects. Clinically, acute multiparametric imaging studies remain challenging. We assessed possible relationships between these factors disease outcome experimental cerebral ischemia-reperfusion, using translational MRI, behavioral testing multi-model inference analyses. Male female rats (N = 60) were...
Objectives: Latest trials failed to confirm merits of nebulized amikacin for critically ill patients with nosocomial pneumonia. We studied various and IV antibiotic regimens in a porcine model severe Pseudomonas aeruginosa pneumonia, resistant amikacin, fosfomycin, susceptible meropenem. Design: Prospective randomized animal study. Setting: Animal Research, University Barcelona, Spain. Subjects: Thirty female pigs. Interventions: The animals were receive saline solution (CONTROL); every 6...
Abstract Patients with mono-lateral pneumonia and severe respiratory failure can be positioned in lateral decubitus, the healthy lung dependent, to improve ventilation-perfusion coupling. Oxygenation response this manoeuvre is heterogeneous derecruitment of dependent has not been elucidated. Nine pigs (32.2 ± 1.2 kg) were sedated mechanically ventilated. Mono-lateral right-sided was induced intrabronchial challenge Pseudomonas aeruginosa . After 24 h, lungs recruited animals randomly on...
Background Hypothermia has been associated with therapeutic benefits including reduced mortality and better neurologic outcomes in survivors of cardiac arrest. However, undesirable side effects have reported patients undergoing coronary interventions. Using a large animal model temperature management, we aimed to describe how interferes the vasculature. Methods Results Coronary hemodynamics endothelial function were studied 12 pigs at various core temperatures. Left circumflex artery was...
Endothelial cell dysfunction is the principal cause of several cardiovascular diseases that are increasing in prevalence, healthcare costs, and mortality. Developing a standardized, representative vitro model endothelial fundamental to greater understanding pathophysiology, aiding development novel pharmacological therapies. We subjected human umbilical vein cells (HUVECs) different periods nutrient deprivation or doses H2O2 represent starvation elevated oxidative stress, respectively,...
Large animal models of acute myocardial infarction (AMI) play a crucial role in translating novel therapeutic approaches to patients as denoted by their use the right-before-human testing platform. At present, porcine model AMI is used most frequently it mimics human condition and its anatomopathological features accurately. We want describe to, share with, translational research community our experience how different anaesthetic protocols (sevoflurane, midazolam,...
Abstract Background X‐ray digital subtraction angiography (DSA) is the imaging modality for peri‐procedural guidance and treatment evaluation in (neuro‐) vascular interventions. Perfusion image construction from DSA, as a means of quantitatively depicting cerebral hemodynamics, has been shown feasible. However, quantitative property perfusion DSA not well studied. Purpose To comparatively study independence deconvolution‐based with respect to varying injection protocols, its sensitivity...
Introduction The lower rates of cardiovascular disease in Southern Europe could be partially explained by the low prevalence lipid-rich atheroma plaques. Consumption certain foods affects progression and severity atherosclerosis. We investigated whether isocaloric inclusion walnuts within an atherogenic diet prevents phenotypes predicting unstable plaque a mouse model accelerated Methods Apolipoprotein E-deficient male mice (10-week-old) were randomized to receive control (9.6% energy as...
<b>Introduction:</b><i>Streptococcus pneumoniae</i> is the leading cause of community-acquired pneumonia (CAP). β-lactams combined with corticosteroids and macrolides may reduce treatment failure rate mortality CAP. <b>Objective:</b> We aimed to compare efficacy different antibiotic combinations adjunct therapy corticosteroids, in a swine pneumococcal model. <b>Methods:</b> Thirty pigs (32±3kg) were mechanically ventilated for 76h. At diagnosis after intrabronchial inoculation <i>S. 19A,...
Current guidelines recommend vancomycin and linezolid as first-line agents against methicillin-resistant Staphylococcus aureus (MRSA) nosocomial pneumonia. Telavancin is a potential new therapeutic alternative, specifically in monomicrobial MRSA This study compared the efficacies of telavancin versus porcine model severe In 18 mechanically ventilated pigs (32.11 ± 1.18 kg), 75 ml 106 CFU/ml was administered into each pulmonary lobe. After onset pneumonia, were randomized three groups:...
The rising frequency of multidrug-resistant and extensively drug-resistant (MDR/XDR) pathogens is making more frequent the inappropriate empirical antimicrobial therapy (IEAT) in nosocomial pneumonia, which associated with increased mortality. We aim to determine short-term benefits appropriate treatment (AEAT) ceftolozane/tazobactam (C/T) compared IEAT piperacillin/tazobactam (TZP) MDR Pseudomonas aeruginosa pneumonia. Twenty-one pigs pneumonia caused by an XDR P. strain (susceptible C/T...
<b>Introduction:</b> Mucus retention is frequent in critically-ill patients on mechanical ventilation (MV). The in-exsufflator (MI-E) machine, namely Cough Assist, aims at improving mucus clearance. <b>Objectivs:</b> To evaluate the effects of different combinations MI-E pressure levels hemodynamic parameters <b>Methods:</b> We included 5 pigs MV. Upon use MI-E, animals randomly received 8 inspiratory-expiratory pressures (cmH2O). Each treatment was applied 4 times during brief...