A5004040733- Ion channel regulation and function
- Neuroscience of respiration and sleep
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- High Altitude and Hypoxia
- Hemoglobin structure and function
- Cardiomyopathy and Myosin Studies
- Receptor Mechanisms and Signaling
- Ion Transport and Channel Regulation
- Nitric Oxide and Endothelin Effects
- Sleep and Wakefulness Research
- Hormonal Regulation and Hypertension
- Blood Pressure and Hypertension Studies
- Neurobiology and Insect Physiology Research
- Sodium Intake and Health
- Nicotinic Acetylcholine Receptors Study
- Biochemical Analysis and Sensing Techniques
- Medicinal Plants and Bioactive Compounds
- Cardiac Ischemia and Reperfusion
- Cell Adhesion Molecules Research
- Parathyroid Disorders and Treatments
- Metabolomics and Mass Spectrometry Studies
- Herbal Medicine Research Studies
Instituto de Biomedicina y Genética Molecular de Valladolid
2015-2024
Consejo Superior de Investigaciones Científicas
2014-2024
Universidad de Valladolid
2015-2024
Instituto de Salud Carlos III
2024
Universitat de Barcelona
2005-2010
Unidades Centrales Científico-Técnicas
2009
University of Maryland, Baltimore
1994-2007
Universidad de Sevilla
1989
The ionic currents of carotid body type I cells and their possible involvement in the detection oxygen tension ( P O 2 ) arterial blood are unknown. electrical properties these were studied with whole-cell patch clamp technique, hypothesis that conductances can be altered by changes was tested. results show have voltage-dependent sodium, calcium, potassium channels. Sodium calcium unaffected a decrease from 150 to 10 millimeters mercury, whereas, same experimental protocol, reversibly...
Excitation-contraction coupling was studied in mammalian cardiac cells which the opening probability of L-type calcium (Ca2+) channels reduced. Confocal microscopy during voltage-clamp depolarization revealed distinct local transients concentration intracellular ions ([Ca2+]i). When voltage varied, latency to occurrence and relative [Ca2+]i varied as predicted if Ca2+ release from sarcoplasmic reticulum (SR) linked tightly flux through but not that Na-Ca exchanger or average [Ca2+]i. Voltage...
Gram-negative bacterial infections are accompanied by inflammation and somatic or visceral pain. These symptoms generally attributed to sensitization of nociceptors inflammatory mediators released immune cells. Nociceptor during occurs through activation the Toll-like receptor 4 (TLR4) signalling pathway lipopolysaccharide (LPS), a toxic by-product lysis. Here we show that LPS exerts fast, membrane delimited, excitatory actions via TRPA1, transient potential cation channel is critical for...
1. Cytosolic free calcium ion concentration ([Ca2+]i) and whole‐cell L‐type Ca2+ channel currents were measured during excitation‐contraction (E‐C) coupling in single voltage‐clamped rat cardiac ventricular cells. The measurements used to compute the total cellular efflux of ions through sarcoplasmic reticulum (SR) release channels (FSR,rel) influx via (FICa). 2. FSR,rel was elicited by depolarizing voltage‐clamp pulses 200 ms duration membrane potentials from ‐30 +80 mV. Over this range,...
The hypothesis that changes in environmental O2 tension (pO2) could affect the ionic conductances of dissociated type I cells carotid body was tested. Cells were subjected to whole-cell patch clamp and currents recorded a control solution with normal pO2 (pO2 = 150 mmHg) 3-5 min after exposure same lower pO2. Na Ca unaffected by lowering 10 mmHg, however, all studied (n 42) hypoxia produced reversible reduction K current. In 14 exposed mmHg peak current amplitude decreased 35 +/- 8% value....
1. Confocal microscopy and the fluorescent Ca2+ indicator fluo‐3 (K+ salt) were used to measure cytosolic free calcium ion concentration ([Ca2+]) during excitation‐contraction (E‐C) coupling in single, voltage‐clamped, rat cardiac ventricular cells. 2. Local [Ca2+]i transients measured nearly simultaneously different, separate, subcellular volumes of approximately 2.0 microns 3. During depolarization, local distinctly different from each other whole‐cell transients. These differences...
Voltage-gated K+ (KV) channels are protein complexes composed of ion-conducting integral membrane α subunits and cytoplasmic modulatory β subunits. The differential expression association seems to contribute significantly the complexity heterogeneity KV in excitable cells, their functional heterologous systems provides a tool study regulation at molecular level. Here, we have studied effects Kvβ1.2 coexpression on properties Shaker Kv4.2 channel subunits, which encode rapidly inactivating...
Hypoxic inhibition of large-conductance Ca(2+)-dependent K(+) channels (maxiK) rat carotid body type I cells is a well-established fact. However, the molecular mechanisms such and role these in process hypoxic transduction remain unclear. We have examined interaction O(2) with maxiK exploring effect hypoxia on currents recorded whole-cell inside-out configuration patch-clamp technique. Hypoxia inhibits channel activity both excised membrane patches. This strongly voltage- Ca(2+)-dependent,...
Objective— Phenotypic modulation of vascular smooth muscle cells has been associated with a decreased expression all voltage-dependent potassium channel (Kv)1 encoding genes but Kcna3 (which encodes Kv1.3 channels). In fact, upregulation currents seems to be important modulate proliferation mice femoral in culture. This study was designed explore if these changes Kv1 pattern constituted landmark phenotypic across beds and investigate the mechanisms involved proproliferative function...
Ionic currents of enzymatically dispersed type I and II cells the carotid body have been studied using whole cell variant patch-clamp technique. Type only a tiny, slowly activating outward potassium current. By contrast, in every chemoreceptor we found (a) sodium, (b) calcium, (c) currents. The sodium current has fast activation time course an threshold at approximately -40 mV. At all voltages inactivation follows single exponential course. constant is 0.67 ms 0 Half steady state occurs...
As there are wide interspecies variations in the molecular nature of O(2)-sensitive Kv channels arterial chemoreceptors, we have characterized expression these and their hypoxic sensitivity mouse carotid body (CB). CB chemoreceptor cells were obtained from a transgenic expressing green fluorescent protein (GFP) under control tyrosine hydroxylase (TH) promoter. Immunocytochemical identification TH cell cultures reveals good match with GFP-positive cells. Furthermore, show an increase...
1. The electrical properties of chemoreceptor cells from neonatal rat and adult rabbit carotid bodies (CBs) are strikingly different. These differences have been suggested to be developmental and/or species related. To distinguish between the two possibilities, whole‐cell configuration patch‐clamp technique was used characterize ionic currents present in isolated CBs. Since hypoxia‐induced inhibition O2‐sensitive K+ is considered a crucial step O2 chemoreception, effect hypoxia on cell also...
Rabbit carotid body (CB) chemoreceptor cells possess a fast‐inactivating K + current that is specifically inhibited by hypoxia. We have studied the expression of Kvα subunits, which might be responsible for this current. RT‐PCR experiments identified Kv1.4, Kv3.4, Kv4.1 and Kv4.3 mRNAs in rabbit CB. There was no Kv3.3 or Kv4.2 transcripts. Immunocytochemistry with antibodies to tyrosine hydroxylase (anti‐TH) specific Kv subunits revealed Kv3.4 cells, while Kv1.4 only found nerve fibres. mRNA...
K + currents recorded from adult rabbit chemoreceptor cells are reversibly inhibited on lowering the pO 2 in bathing solution. Bath application of a hypoxic TTX‐containing solution revealed that inhibition current by low , proceeds faster than TTX Na currents, apparent t ½ being 3.68 and 7.14 s, respectively. Addition carbon monoxide to gas mixture used equilibrate reversed approx. 70%.
1. Cat carotid bodies were incubated with the precursor [3H]tyrosine to label catecholamine deposits and then mounted in a superfusion chamber which allowed simultaneous collection of released [3H]dopamine (DA) recording action potentials from sinus nerve. 2. Low pH (7.2‐6.6) for periods 10 min produced parallel increase release [3H]DA chemoreceptor discharge. 3. Carotid nerve denervation body 12‐15 days prior experiments did not modify elicited by low pH. 4. Superfusion Ca(2+)‐free,...
Vascular smooth muscle cells (VSMCs) contribute significantly to occlusive vascular diseases by virtue of their ability switch a noncontractile, migratory, and proliferating phenotype. Although the participation ion channels in this phenotypic modulation (PM) has been described previously, changes expression are poorly defined because large molecular diversity. We obtained global portrait channel contractile versus mouse femoral artery VSMCs, explored functional contribution PM most relevant...
Canonical transient receptor potential (TRPC)3 and TRPC6 channels of vascular smooth muscle cells (VSMCs) mediate stretch- or agonist-induced cationic fluxes, contributing to membrane tone. Native TRPC3/C6 can form homo- heterotetrameric complexes, which hinder individual TRPC channel properties. The possibility that the differences in their association pattern may change contribution tone hypertension is unexplored. Functional characterization heterologously expressed showed...
1. Adult rabbit carotid body chemoreceptor cells, enzymatically dispersed and short‐term cultured, exhibit an inactivating outward K+ current that is reversibly inhibited by low PO2. In the present work we have characterized biophysical pharmacological properties of this using whole‐cell voltage clamp recording technique. 2. Inactivating was recorded after blockage Ca2+ currents with extracellular Co2+, Cd2+, or complete washing out channels. 3. The threshold activation about ‐40 mV. Current...
Vascular smooth muscle cells (VSMCs) perform diverse functions that can be classified into contractile and synthetic (or proliferating). All of these fulfilled by the same cell because its capacity phenotypic modulation in response to environmental changes. The resting membrane potential is a key determinant for both proliferating functions. Here, we have explored expression voltage-dependent K+ (Kv) channels (freshly dissociated) (cultured) VSMCs obtained from human uterine arteries...
Essential hypertension involves a gradual and sustained increase in total peripheral resistance, reflecting an increased vascular tone. This change associates with depolarization of myocytes, relies on the expression profile voltage-dependent ion channels (mainly Ca(2+) K(+) channels) that promotes arterial contraction. However, changes and/or modulation (Kv are poorly defined, due to their large molecular diversity bed-specific expression. Here we endeavor characterize functional Kv smooth...