A5063780117- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Immune cells in cancer
- Neuroscience and Neural Engineering
- Viral Infections and Immunology Research
- Analytical Chemistry and Sensors
- Neuroblastoma Research and Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Nitric Oxide and Endothelin Effects
- Graphene and Nanomaterials Applications
- Semiconductor materials and devices
- Medicinal Plants and Bioactive Compounds
- Signaling Pathways in Disease
- Cardiac Arrhythmias and Treatments
- Cardiomyopathy and Myosin Studies
- Electrochemical Analysis and Applications
- Renin-Angiotensin System Studies
- Cardiac Fibrosis and Remodeling
- Analytical Methods in Pharmaceuticals
- Chemical Synthesis and Characterization
- Hydrogen's biological and therapeutic effects
- Sleep and Wakefulness Research
- Cardiac Ischemia and Reperfusion
Universidad de Valladolid
2022-2024
Instituto de Biomedicina y Genética Molecular de Valladolid
2023-2024
Consejo Superior de Investigaciones Científicas
2017-2024
Instituto de Investigaciones Biomédicas Sols-Morreale
2014-2023
Universidad Autónoma de Madrid
2014-2023
Instituto de Salud Carlos III
2019-2021
Centro de Investigación en Red en Enfermedades Cardiovasculares
2019-2021
University of Talca
2020
Resolution of inflammation is orchestrated by specialized proresolving lipid mediators (SPMs), and this would be impaired in some cardiovascular diseases. Among SPMs, resolvins (Rv) have beneficial effects pathologies, but little known about their effect on damage hypertension.Aorta, small mesenteric arteries, heart, peritoneal macrophages were taken from C57BL/6J mice, infused or not with angiotensin II (AngII; 1.44 mg/kg/day, 14 days) presence absence resolvin D2 (RvD2) (100 ng/mice, every...
Experimental evidence indicates that the control of inflammatory response after myocardial infarction is a key strategy to reduce cardiac injury. Cellular damage blood flow restoration in heart promotes sterile inflammation through release molecules activate pattern recognition receptors, among which TLR4 most prominent. Transient regulation activity has been considered one potential therapeutic interventions with greater projection towards clinic. In this regard, characterization an aptamer...
Abstract DREAM, a neuronal calcium sensor protein, has multiple cellular roles including the regulation of Ca 2+ and protein homeostasis. We recently showed that reduced DREAM expression or blockade activity by repaglinide is neuroprotective in Huntington’s disease (HD). Here we used structure-based drug design to guide identification IQM-PC330, which was more potent had longer lasting effects than inhibit vivo HD models. disclosed validated an unexplored ligand binding site, showing Tyr118...
Abstract The interaction of two types fragmented graphene particles (30–160 nm) with human macrophages is studied. Since have significant phagocytic activity, the incorporation into cells has an effect on response to functional polarization stimuli, favoring anti‐inflammatory profile. Incubation foam particles, prepared by chemical vapor deposition, and commercially available nanoplatelet does not affect cell viability when added at concentrations up 100 µg mL −1 ; exhibit differential...
Immune cells have an important role in the tumor-microenvironment. Macrophages may tune immune response toward inflammatory or tolerance pathways. Tumor-associated macrophages (TAM) a string of immunosuppressive functions and they are considered therapeutic target cancer. This study aimed to analyze effects trabectedin, antitumor agent, on tumor-microenvironment through characterization electrophysiological molecular phenotype macrophages. Experiments were performed using whole-cell...
DREAM/KChIP3/calsenilin is a neuronal calcium sensor with multiple functions, including the regulation of A-type outward potassium currents (IA). This effect mediated by interaction between DREAM and KV4 channels it has been shown that small molecules bind to modify channel function. current (IA) responsible fast repolarization neuron action potentials frequency firing. Using surface plasmon resonance assays electrophysiological recordings KV4.3/DREAM channels, we have identified IQM-266 as...
Background and Purpose K V 1.3 channels are expressed in vascular smooth muscle cells (VSMCs), where they contribute to proliferation rather than contraction participate remodelling. also macrophages, assemble with 1.5 (K 1.3/K 1.5), whose activation generates a current. In the ratio is increased by classical (M1). Whether these involved angiotensin II (AngII)‐induced remodelling, whether can modulate macrophage phenotype hypertension, remains unknown. We characterized role of damage...
Local anaesthetics block sodium and a variety of potassium channels. Although previous studies identified residue in the pore signature sequence together with three residues S6 segment as putative binding site, precise molecular basis inhibition Kv channels by local remained unknown. Crystal structures predict that some these point away from central cavity face into drug site called side pockets. Thus, question arises whether is exclusively located or also involves pockets.A systematic...
Abstract Voltage‐dependent potassium channel Kv1.3 plays a key role on T‐cell activation; however, lack of reliable antibodies has prevented its accurate detection under endogenous circumstances. To overcome this limitation, we created Jurkat line with tagged, to determine the expression, location, and changes upon activation native channels. CRISPR‐Cas9 technique was used insert Flag‐Myc peptide at C terminus KCNA3 gene. Basal or activated expression studied using western blot analysis...
Fludarabine (F-ara-A) is a purine analogue commonly used in the treatment of indolent B cell malignancies that interferes with different aspects DNA and RNA synthesis. Kv1.3 K+ channels are membrane proteins involved maintenance homeostasis resting potential cell, thus controlling signaling events, proliferation apoptosis lymphocytes. Here we show F-ara-A inhibits Kv currents human Our data indicate expressed both BL2 Dana lines, although total levels were higher than cells. However, plasma...
The Kv β1.3 subunit modifies the gating and pharmacology of 1.5 channels in a PKC-dependent manner, decreasing channel sensitivity to bupivacaine- quinidine-mediated blockade. Cardiac associate with receptor for activated C kinase 1 (RACK1), different PKC isoforms, resulting formation functional channelosome. aim present study was investigate effects inhibition on bupivacaine quinidine block + channels.HEK293 cells were transfected channels, currents recorded using whole-cell configuration...
Introduction Coronary artery disease (CAD) is the foremost single cause of mortality and disability globally. Patients with type 2 diabetes (T2DM) have a higher incidence CAD, poorer prognosis. The low-grade inflammation associated to T2DM contributes increased morbidity worst outcomes after revascularization. Inflammatory signaling in vasculature supports endothelial dysfunction, leukocyte infiltration, macrophage activation metabolic (MMe) specific phenotype, which could contribute...
KV1.5 channel function is modified by different regulatory subunits. KVβ1.3 subunits assemble with channels and induce a fast incomplete inactivation. Inhibition of PKC abolishes the KVβ1.3-induced inactivation, decreases amplitude current KV1.5–KVβ1.3 modifies their pharmacology likely due to changes in traffic PKC-dependent manner. In order analyze this hypothesis, HEK293 cells were transfected channels, currents recorded whole-cell configuration patch-clamp technique. The presence...