A5036074767- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Estrogen and related hormone effects
- Glycosylation and Glycoproteins Research
- Plant biochemistry and biosynthesis
- Receptor Mechanisms and Signaling
- Microbial Natural Products and Biosynthesis
- Metabolism and Genetic Disorders
- Liver Disease and Transplantation
- Diet, Metabolism, and Disease
- Microbial Metabolism and Applications
- Peroxisome Proliferator-Activated Receptors
- RNA modifications and cancer
- Protein Tyrosine Phosphatases
- Liver Disease Diagnosis and Treatment
- Galectins and Cancer Biology
- Renal Transplantation Outcomes and Treatments
- Graphene and Nanomaterials Applications
- Diet and metabolism studies
- Epigenetics and DNA Methylation
- Lipoproteins and Cardiovascular Health
- Antifungal resistance and susceptibility
- Adenosine and Purinergic Signaling
Universidad Autónoma de Madrid
1989-2023
Instituto de Investigaciones Biomédicas Sols-Morreale
2014-2023
Consejo Superior de Investigaciones Científicas
1989-2023
Universitat de Barcelona
2023
Instituto de Investigación en Ciencias de la Alimentación
2017
Hospital General Universitario Gregorio Marañón
2016
Centro de Investigación Biomédica en Red
2015
Instituto de Salud Carlos III
2015
Presbyterian Hospital
2015
Cornell University
2015
Objective— Although it is accepted that macrophage glycolysis upregulated under hypoxic conditions, not known whether this linked to a similar increase in proinflammatory activation and specific energy demands regulate cell viability the atheromatous plaque. Approach Results— We studied interplay between metabolism, polarization, context of atherosclerosis. Cultured human murine macrophages an vivo model atherosclerosis were used evaluate mechanisms underlying metabolic inflammatory activity...
Dehydroepiandrosterone (DHEA) exhibits peak adrenal secretion in the fetus at term and around age 30 yr adult. Levels then progressively decline, which is associated with decreased levels of testosterone, dihydrotestosterone, estrogen peripheral tissues. DHEA supplementation postmenopausal women increases bone formation density, an effect mainly attributed to conversion sex hormones. In this study, we tested for direct effects on androgen (AR) (ER) receptors. bound AR a Ki 1 microM, was...
A novel anti-influenza virus compound, flutimide, was identified in extracts of a recently fungal species, Delitschia confertaspora (F. Pelaez, J.D. Polishook, M. Valldosera, and J.Guarro, Mycotaxon 50:115-122, 1994). The substituted 2,6-diketopiperazine, selectively inhibited the cap-dependent transcriptase influenza B viruses had no effect on activities other polymerases. Similar to 4-substituted 2,4-dioxobutanoic acids, series inhibitors which we described previously (J. Tomassini, H....
Inhibition of protein tyrosine phosphatase 1B (PTP1B) has been suggested as an attractive target to improve insulin sensitivity in different cell types. In the present work, we have investigated effect PTP1B deficiency on response human and murine macrophages. Using vitro vivo approaches mice silencing macrophages with specific siRNAs, demonstrated that increases effects pro-inflammatory stimuli both rodent at time decreases alternative stimulation. Moreover, absence induces a loss viability...
Macrophages are present in a large variety of locations, playing distinct functions that determined by its developmental origin and the nature activators microenvironment. Macrophage activation can be classified as pro-inflammatory (M1 polarization) or anti-inflammatory-pro-resolution-deactivation (M2), these profiles coexisting course immune response relevant functional role onset inflammation (Figure 1). Several groups have analysed metabolic aspects associated with macrophage to answer...
We have studied the effect of Vasoactive Intestinal Peptide (VIP) on glycogenolysis and gluconeogenesis (as measured by conversion [U-14C]pyruvate into glucose) in hepatocytes isolated from fed rats. The influence VIP glycogen phosphorylase alpha pyruvate kinase activities, as well cAMP levels, was also evaluated. In addition, possible antagonism insulin these VIP-mediated effects investigated. enhanced both a dose-dependent manner. At 10(-6) M VIP, processes were increased 2-fold compared...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTZaragozic Acids D and D2: Potent Inhibitors of Squalene Synthase Ras Farnesyl-Protein TransferaseClaude Dufresne, Kenneth E. Wilson, Sheo Bux Singh, Deborah L. Zink, James D. Bergstrom, Rew, Jon Polishook, Maria Meinz, Leeyuan Huang, Keith C. Silverman, Russell B. Lingham, Marina Mojena, Carmen Cascales, Fernando Pelaéz, Jackson GibbsCite this: J. Nat. Prod. 1993, 56, 11, 1923–1929Publication Date (Print):November 1, 1993Publication History...
The concentration of fructose 2,6-bisphosphate and the activity 6-phosphofructo-2-kinase are increased after infection chick-embryo fibroblasts with Rous sarcoma virus, or a temperature-sensitive mutant this virus at permissive, but not non-permissive, temperature. This is observed transformation by retroviruses carrying either v-src v-fps, v-mil and/or v-myc, oncogenes. Comparison effects those phorbol myristate acetate on suggests that both result from stimulation step which rate-limiting...
The effects of L-796,449 (3-chloro-4-(3-(3-phenyl-7-propylbenzofuran-6-yloxy)propylthio)phenylacetic acid; referred to henceforth as compound G), a thiazolidinedione-unrelated peroxisome proliferator activated-receptor-γ (PPAR-γ) agonist, on early signaling in lipopolysaccharide-activated RAW 264.7 macrophages were analyzed and compared with those elicited by 15-deoxy-Δ<sup>12,14</sup>-prostaglandin J<sub>2</sub> the thiazolidinedione rosiglitazone. Compound G inhibited activation nuclear...
The addition of chlorpropamide to hepatocytes isolated from fed rats raised the cellular concentration fructose-2,6-bisphosphate (F-2,6-P2), a regulatory metabolite that plays relevant role in control hepatic glucose metabolism. effect was dose dependent; statistically significant increase already seen at 0.2 mM sulfonylurea. accumulation F-2,6-P2 caused by (1 mM) parallel stimulation L-lactate production (36.6 +/- 4.8 versus 26.1 2.6 mumol lactate/g cells X 20 min; N = 5, P less than 0.05)...
Glutamine caused a dose-dependent decrease in fructose 2,6-bisphosphate concentration both HeLa cells and chick-embryo fibroblasts. The effect was complete within 15 min cells, but required more than 9 h the Half-maximal effects were obtained with 0.1-0.3 mM-glutamine. In fibroblasts, not glutamine induced time-dependent activity of phosphofructokinase-2, which correlated 2,6-bisphosphate. decreased glycolytic flux by about 25% only difference response between two types might correspond to...
In recent years, the central role of cell bioenergetics in regulating immune function and fate has been recognized, giving rise to interest immunometabolism, an area research focused on interaction between metabolic regulation function. Thus, early changes associated with polarization macrophages into pro-inflammatory or pro-resolving cells under different stimuli have characterized. Tumor-associated are among most abundant tumor microenvironment; however, it exists unmet need study effect...
The effect of pre-existent hepatic NO synthesis on liver injury induced by lipopolysaccharide was studied in animals carrying a nitric oxide synthase-2 (NOS-2) transgene under the control phosphoenolpyruvate carboxykinase (PEPCK) promoter. These expressed NOS-2 cells fasting conditions. Lipopolysaccharide-induced D-galactosamine-conditioned mice, which enhanced notably endotoxin liver, impaired expressing NOS-2. This protection against inflammatory damage dependent and caused an inhibition...
<sup>18</sup>F-FDG accumulates in glycolytically active tissues and is known to concentrate that are rich activated macrophages. In this study, we tested the hypotheses human granulocyte-macrophage colony-stimulating factor (GM-CSF), a clinically used cytokine, increases macrophage glycolysis deoxyglucose uptake vitro acutely enhances within inflamed such as atherosclerotic plaques vivo. <b>Methods:</b> experiments were conducted on macrophages whereby inflammatory activation of radiolabeled...
Atherosclerosis is a cardiovascular disease caused mainly by dyslipidemia and characterized the formation of an atheroma plaque chronic inflammation. Proprotein convertase subtilisin/kexin type 9 (PCSK9) protease that induces degradation LDL receptor (LDLR), which contributes to increased levels cholesterol progress atherosclerosis. Given macrophages are relevant components lipidic inflammatory environment atherosclerosis, we studied effects PCSK9 treatment on human macrophages. Our data...
The influence of tumour promoters and growth factors on glycolysis fructose‐2,6‐bisphosphate concentration was studied in isolated mouse spleen lymphocytes purified B‐cells. intracellular fructose 2,6‐bisphosphate the rate lactate release were increased 2–3‐fold exposed to active phorbol esters, mitogenic lectins, interleukin 4 or lipopolysaccharide. maximal effect observed after 1 h exposure. In these cells hexose 6‐phosphates 2‐fold 6‐phosphofructo‐2‐kinase activity remained unchanged...
Melanomas are heterogeneous and aggressive tumors, one of the worse in prognosis. Melanoma subtypes follow distinct pathways until terminal oncogenic transformation. Here, we have evaluated a series molecules that exhibit potent cytotoxic effects over murine human melanoma cell lines B16F10 MalMe-3M, respectively, both ex vivo animals carrying these cells. Ex mechanistic studies on molecular targets involved growth, migration viability were cultured cells treated with drugs which exhibited...
Tumor-associated macrophages (TAMs) play a crucial role in suppressing the immunosurveillance function of immune system that prevents tumor growth. Indeed, can also be targeted by different chemotherapeutic agents improving action over checkpoints to fight cancer. Here we describe effect trabectedin and lurbinectedin on human macrophage cell viability function.Blood monocytes from healthy donors were differentiated into exposed stimuli promoting functional polarization differentiation...
Abstract The interaction of two types fragmented graphene particles (30–160 nm) with human macrophages is studied. Since have significant phagocytic activity, the incorporation into cells has an effect on response to functional polarization stimuli, favoring anti‐inflammatory profile. Incubation foam particles, prepared by chemical vapor deposition, and commercially available nanoplatelet does not affect cell viability when added at concentrations up 100 µg mL −1 ; exhibit differential...
Immune cells have an important role in the tumor-microenvironment. Macrophages may tune immune response toward inflammatory or tolerance pathways. Tumor-associated macrophages (TAM) a string of immunosuppressive functions and they are considered therapeutic target cancer. This study aimed to analyze effects trabectedin, antitumor agent, on tumor-microenvironment through characterization electrophysiological molecular phenotype macrophages. Experiments were performed using whole-cell...