A5010555621- Immune cells in cancer
- Adenosine and Purinergic Signaling
- Peroxisome Proliferator-Activated Receptors
- Galectins and Cancer Biology
- Genomics, phytochemicals, and oxidative stress
- Cancer, Hypoxia, and Metabolism
- Immune Response and Inflammation
- Eicosanoids and Hypertension Pharmacology
- Adipose Tissue and Metabolism
- Protein Tyrosine Phosphatases
- Gastroesophageal reflux and treatments
- Genetics and Neurodevelopmental Disorders
- Neuroendocrine Tumor Research Advances
- Epigenetics and DNA Methylation
- Asthma and respiratory diseases
- Natural product bioactivities and synthesis
- interferon and immune responses
- NF-κB Signaling Pathways
- Immune Cell Function and Interaction
- Neuropeptides and Animal Physiology
- S100 Proteins and Annexins
- Inflammatory mediators and NSAID effects
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
Universidad Autónoma de Madrid
2011-2018
Instituto de Investigaciones Biomédicas Sols-Morreale
2011-2018
Centro de Investigación Biomédica en Red
2015
Instituto de Salud Carlos III
2015
Cornell University
2015
Presbyterian Hospital
2015
Universidad de Las Palmas de Gran Canaria
2014
Massachusetts General Hospital
2014
Consejo Superior de Investigaciones Científicas
2011-2013
Objective— Although it is accepted that macrophage glycolysis upregulated under hypoxic conditions, not known whether this linked to a similar increase in proinflammatory activation and specific energy demands regulate cell viability the atheromatous plaque. Approach Results— We studied interplay between metabolism, polarization, context of atherosclerosis. Cultured human murine macrophages an vivo model atherosclerosis were used evaluate mechanisms underlying metabolic inflammatory activity...
Inhibition of protein tyrosine phosphatase 1B (PTP1B) has been suggested as an attractive target to improve insulin sensitivity in different cell types. In the present work, we have investigated effect PTP1B deficiency on response human and murine macrophages. Using vitro vivo approaches mice silencing macrophages with specific siRNAs, demonstrated that increases effects pro-inflammatory stimuli both rodent at time decreases alternative stimulation. Moreover, absence induces a loss viability...
The activation of immune cells in response to a pathogen involves succession signaling events leading gene and protein expression, which requires metabolic changes match the energy demands. profile associated with MAPK cascade (ERK1/2, p38, JNK) macrophages was studied, effect its inhibition on specific pattern LPS stimulation characterized. A [1,2-[(13)C](2)]glucose tracer-based metabolomic approach used examine flux distribution these after MEK/ERK inhibition. Bioinformatic tools were...
Potassium channels modulate macrophage physiology. Blockade of voltage-dependent potassium (Kv) by specific antagonists decreases cytokine production and inhibits proliferation. In the presence aspirin, acetylated cyclooxygenase-2 loses activity required to synthesize PGs but maintains oxygenase produce 15R-HETE from arachidonate. This intermediate product is transformed via 5-LOX into epimeric lipoxins, termed 15-epi-lipoxins (15-epi-lipoxin A4 [e-LXA4]). Kv have been proposed as...
Extracellular nucleotides have been recognized as important modulators of inflammation via their action on specific pyrimidine receptors (P2). This regulation coexists with the temporal framework proinflammatory and proresolution mediators released by cells involved in inflammatory response, including macrophages. Under conditions, expression cyclooxygenase-2 leads to release large amounts PGs, such PGE2, that exert effects through EP other intracellular targets. The effect these PGs P2...
Protein tyrosine phosphatase 1B (PTP1B) is widely expressed in mammalian tissues, particular immune cells, and plays a pleiotropic role dephosphorylating many substrates. Moreover, PTP1B expression enhanced response to pro-inflammatory stimuli different cell stressors. Taking advantage of the use mice deficient we have investigated effect γ-radiation these animals found lethality decreased respiratory exchange ratio vs. corresponding wild type animals. Using bone-marrow derived macrophages...
The nucleotide uridine trisphosphate (UTP) released to the extracellular milieu acts as a signaling molecule via activation of specific pyrimidine receptors (P2Y). P2Y are G protein-coupled expressed in many cell types. These mediate several responses and they involved intracellular calcium mobilization. We investigated role prostanoid PGE 2 mouse embryonic fibroblasts (MEFs), since these cells different ontogenic physiopathological processes, among them is tissue repair following...
Abstract Immune activation rapidly and substantially enhances metabolic outputs. Macrophage is followed by rapid changes in nutrient flux, which also seems to be necessary for immune activation, indicating that signals produced cells might directly regulate their metabolism. Macrophages respond a variety of extracellular stimuli developing patterns gene expression encompass linked the programs are not fully understood. can activated two opposite end-points: M1-polarization accomplished...