A5006364089- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Natural product bioactivities and synthesis
- Mitochondrial Function and Pathology
- Microbial Metabolic Engineering and Bioproduction
- Metabolism, Diabetes, and Cancer
- Epigenetics and DNA Methylation
- Acute Myeloid Leukemia Research
- Bacterial Genetics and Biotechnology
- Phytochemistry and Biological Activities
- Immune cells in cancer
- Pancreatic function and diabetes
- Bioactive Compounds and Antitumor Agents
- Diet and metabolism studies
- Lung Cancer Treatments and Mutations
- Legume Nitrogen Fixing Symbiosis
- Glaucoma and retinal disorders
- Cancer-related Molecular Pathways
- Ocular Surface and Contact Lens
- Medicinal Plants and Neuroprotection
- Biochemical and Molecular Research
- Bioinformatics and Genomic Networks
- Pharmacological Effects of Natural Compounds
- Pesticide and Herbicide Environmental Studies
- Plant biochemistry and biosynthesis
Universitat de Barcelona
2016-2025
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2018-2025
Instituto de Salud Carlos III
2018-2025
Institut de Biomedicina de la Universitat de Barcelona
2013-2024
Institute for Research in Biomedicine
2011-2024
Consejo Superior de Investigaciones Científicas
2008-2023
Instituto de Investigaciones Biomédicas Sols-Morreale
2023
Hospital Sant Joan de Déu Barcelona
2008-2022
Clínica Diagonal
2022
University of Banja Luka
2018-2020
The occurrence and the further spread of high-level glycopeptide-resistant, vanA-positive Enterococcus faecium strains outside hospitals have been investigated. We could isolate such bacteria directly from thawing liquids commercially produced frozen poultry (chickens, turkeys; no data on previous feeding with avoparcin were available). In 5 13 samples raw minced meat pigs originating different butcher's shops, glycopeptide-resistant E. (VanA type) be detected after overnight broth...
Metabolomics has become a crucial phenotyping technique in range of research fields including medicine, the life sciences, biotechnology and environmental sciences. This necessitates transfer experimental information between groups, as well potentially to publishers funders. After initial efforts metabolomics standards initiative, minimum reporting were proposed which included concepts for databases. Built by community, infrastructure are still needed allow storage, exchange, comparison...
In solid tumors, cancer stem cells (CSCs) can arise independently of epithelial-mesenchymal transition (EMT). spite recent efforts, the metabolic reprogramming associated with CSC phenotypes uncoupled from EMT is poorly understood. Here, by using metabolomic and fluxomic approaches, we identify major profiles that differentiate metastatic prostate epithelial CSCs (e-CSCs) non-CSCs expressing a stable EMT. We have found e-CSC program in our cellular model characterized high plasticity energy...
Abstract Acute myeloid leukemia remains difficult to treat due strong genetic heterogeneity between and within individual patients. Here, we show that Pyruvate dehydrogenase kinase 1 (PDK1) acts as a targetable determinant of different metabolic states in acute (AML). PDK1 low AMLs are OXPHOS-driven, enriched for leukemic granulocyte-monocyte progenitor (L-GMP) signatures, associated with FLT3 -ITD NPM1 cyt mutations. high however OXPHOS , wild type stemness signatures. Metabolic can even...
Chronic myeloid leukemia cells contain a constitutively active Bcr-Abl tyrosine kinase, the target protein of Gleevec (STI571) phenylaminopyrimidine class kinase inhibitor. Here we provide evidence for metabolic phenotypic changes in cultured K562 human blast after treatment with increasing doses STI571 using [1,2-13C2]glucose as single tracer and biological mass spectrometry. In response to 0.68 6.8 microm STI571, proliferation Bcr-Abl-positive showed 57% 74% decrease, respectively, whereas...
Metabolomic characteristics in boar spermatozoa were studied using [1,2‐ 13 C 2 ]glucose and mass isotopomer analysis. In spermatozoa, glycolysis was the main pathway of glucose utilization producing lactate/pyruvate, whereas no gluconeogenesis seen. Slight glycogen synthesis through direct some incorporation pyruvate into Krebs cycle also took place. Neither RNA ribose‐5‐phosphate nor fatty acid from occurred despite detection dehydrogenase activity. contrast to known metabolic activities...
The pentose phosphate pathway is a fundamental metabolic that provides cells with ribose and NADPH required for anabolic reactions - synthesis of nucleotides fatty acids maintenance intracellular redox homeostasis. It plays key role in tumor reprogramming has been reported to be deregulated different types tumors. Herein, we silenced the most important enzymes this glucose-6-phosphate dehydrogenase (G6PD) transketolase (TKT) human breast cancer cell line MCF7. We demonstrated inhibition...
Abstract Metabolic programs can differ substantially across genetically distinct subtypes of acute myeloid leukemia (AML). These are not static entities but change swiftly as a consequence extracellular changes or in response to pathway-inhibiting drugs. Here, we uncover that AML patients with FLT3 internal tandem duplications ( -ITD + ) characterized by high expression succinate-CoA ligases and activity mitochondrial electron transport chain (ETC) complex II, thereby driving respiration...
The fermented extract of wheat germ, trade name Avemar, is a complex mixture biologically active molecules with potent anti-metastatic activities in various human malignancies. Here we report the effect Avemar on Jurkat leukemia cell viability, proliferation, cycle distribution, apoptosis, and activity key glycolytic/pentose enzymes that control carbon flow for nucleic acid synthesis. cytotoxic IC50 concentration tumor cells 0.2 mg/ml, increasing doses crude powder inhibit proliferation...
The activation of immune cells in response to a pathogen involves succession signaling events leading gene and protein expression, which requires metabolic changes match the energy demands. profile associated with MAPK cascade (ERK1/2, p38, JNK) macrophages was studied, effect its inhibition on specific pattern LPS stimulation characterized. A [1,2-[(13)C](2)]glucose tracer-based metabolomic approach used examine flux distribution these after MEK/ERK inhibition. Bioinformatic tools were...
Metabolic reprogramming, a crucial cancer hallmark, shifts metabolic pathways such as glycolysis, tricarboxylic acid cycle or lipogenesis, to enable the growth characteristics of cells. Here, we provide evidence that transketolase-like 1 (TKTL1) orchestrates aerobic fatty and nucleic synthesis, glutamine metabolism, protection against oxidative stress cell proliferation. Furthermore, silencing TKTL1 reduced levels sphingolipids lactosylceramide (a sphingolipid regulating survival,...
Antiviral signaling, immune response and cell metabolism are dysregulated by SARS-CoV-2, the causative agent of COVID-19. Here, we show that SARS-CoV-2 accessory proteins ORF3a, ORF9b, ORF9c ORF10 induce a significant mitochondrial metabolic reprogramming in A549 lung epithelial cells. While induced largely overlapping transcriptomes, ORF3a distinct transcriptome, including downregulation numerous genes with critical roles function morphology. On other hand, all four ORFs altered dynamics...
Summary A comparison was made of the effect glyphosate (Roundup ® Plus), a post‐emergency applied herbicide, and Harness GTZ, pre‐emergency on rhizobacterial communities genetically modified NK603 glyphosate‐tolerant maize. The potential monitored by direct amplification, cloning sequencing soil DNA encoding 16S rRNA, hybridization to commercially available genome‐wide microarrays from bacterium Streptomyces coelicolor , high‐throughput pyrosequencing bacterial coding for rRNA hypervariable...
The fermented wheat germ extract with standardized benzoquinone composition has potent tumor propagation inhibitory properties. authors show that this induces profound metabolic changes in cultured MIA pancreatic adenocarcinoma cells when the [1,2- 13 C 2 ]glucose isotope is used as single tracer biologic gas chromatography–mass spectrometry. treated 0.1, 1, and 10 mg/mL showed a dose-dependent decrease cell glucose consumption, uptake of into ribosomal RNA (2.4%, 9.4%, 28.0%), release CO ....
Green tea and grape phenolics inhibit cancer growth modulate cellular metabolism. Targeting the tumor metabolic profile is a novel therapeutic approach to cell proliferation. Therefore, we treated human colon adenocarcinoma HT29 cells with phenolic compound epicatechin gallate (ECG), one of main catechins in green most important catechin extracts, evaluated its antiproliferation effects. ECG reduced viability induced apoptosis, necrosis, S phase arrest cells. Later, biochemical...
Epithelial-mesenchymal-transition promotes intra-tumoral heterogeneity, by enhancing tumor cell invasiveness and promoting drug resistance. We integrated transcriptomic data for two clonal subpopulations from a prostate cancer line (PC-3) into genome-scale metabolic network model to explore their differences potential vulnerabilities. In this dual model, PC-3/S cells express markers display high low metastatic potential, while PC-3/M present the opposite phenotype higher proliferative rate....
Background: Decompression is an approved alternative to cystectomy in the treatment of jaw cysts.This study aimed evaluate its effectiveness as initial procedure, well factors with potential influence outcome.Material and Methods: frequency decompression was analysed, whether completed one session or followed by enucleation at Division Oral Surgery Orthodontics, Department Dental Medicine Health, Medical University Graz, from 2005 2015.Further analysis focussed on potentially influencing...
The chronic effects of metformin on liver gluconeogenesis involve repression the G6pc gene, which is regulated by carbohydrate-response element-binding protein through raised cellular intermediates glucose metabolism. In this study we determined candidate mechanisms lowers 6-phosphate (G6P) in mouse and rat hepatocytes challenged with high or gluconeogenic precursors. Cell loads therapeutic range lowered cell G6P but not ATP decreased mRNA at glucose. lowering was mimicked a complex 1...
Abstract Objectives Human tear analysis holds promise for biomarker discovery, but its clinical utility is hindered by the lack of standardized reference values, limiting interindividual comparisons. This study aimed at developing a protocol normalizing metabolomic data from human tears, enhancing potential identification. Methods Tear profiling was conducted on 103 donors (64 females, 39 males, aged 18–82 years) without ocular pathology, using AbsoluteIDQ™ p180 Kit targeted metabolomics. A...