A5062443901- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Bee Products Chemical Analysis
- Erythrocyte Function and Pathophysiology
- Reproductive Physiology in Livestock
- Ovarian function and disorders
- Veterinary Equine Medical Research
- Male Reproductive Health Studies
- Insect and Arachnid Ecology and Behavior
- Medical research and treatments
- Vector-Borne Animal Diseases
- Animal Behavior and Welfare Studies
- Pancreatic function and diabetes
- Proteins in Food Systems
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Hormonal and reproductive studies
- Genetic and phenotypic traits in livestock
- Reproductive biology and impacts on aquatic species
Zoological Society of London
2002-2020
Universitat Autònoma de Barcelona
2003-2009
Universidad de Las Palmas de Gran Canaria
2002
Royal Veterinary College
2002
Metabolomic characteristics in boar spermatozoa were studied using [1,2‐ 13 C 2 ]glucose and mass isotopomer analysis. In spermatozoa, glycolysis was the main pathway of glucose utilization producing lactate/pyruvate, whereas no gluconeogenesis seen. Slight glycogen synthesis through direct some incorporation pyruvate into Krebs cycle also took place. Neither RNA ribose‐5‐phosphate nor fatty acid from occurred despite detection dehydrogenase activity. contrast to known metabolic activities...
Incubation of boar spermatozoa in Krebs-Ringer-Henseleit medium with either 10 mM lactate or citrate induced a fast and robust increase the intracellular levels ATP both cases, which reached peak after 30 sec incubation. Utilization resulted export CO(2) to extracellular medium, indicating that substrates were metabolized through Krebs cycle. generation lactate, was inhibited presence phenylacetic acid. This indicates is produced pyruvate carboxylase step. In addition, there also significant...
A series of experiments was set up to investigate the effect different cooling rates on boar sperm cryosurvival using cryomicroscopy. The protocols were split into two stages: (i) from +5 degrees C -5 and (ii) -50 C. Fluorescent probes (SYBR14 propidium iodide) used monitor plasma membrane integrity during entire process. Cooling in range 3 min(-1) 12 did not cause significant damage between C; however, spermatozoa cooled at 24 slightly damaged. Motility particularly sensitive variations...
Abstract Incubation of boar sperm from fresh ejaculates in a minimal medium with 10 mM glucose induced fast and intense activation glycolysis, as indicated by the observed increases intracellular levels 6‐phosphate (G 6‐P) ATP rate formation extracellular L ‐lactate. The effect was much more than that fructose, sorbitol, mannose. greater utilization related to sensitivity hexokinase when compared other monosaccharides. Thus, presence 0.5 total activity supernatants extracts 1.7 ± 0.1 mIU/mg...
Contents In this work the role of energy substrates in maintenance boar‐sperm survival during storage at 15–17°C was tested. For purpose, boar spermatozoa were stored several defined media with separate combinations a monosaccharide, glucose and non‐monosaccharide, either citrate or lactate, substrates. Our results indicate that medium containing highest concentration together low lactate levels most suitable to maintain sperm quality for 168 h 15–17°C. This confirmed after observation...
The presence of a high‐ K m hexokinase activity was tested in both dog and boar spermatozoa. Hexokinase kinetics from extracts showed the specific (dog‐sperm glucokinase‐like protein, DSGLP), range glucose concentrations 4–10 mM, whereas sperm did not show any DSGLP activity. Furthermore, dog‐sperm cells, but those boar, protein which specifically reacted against rat‐liver anti‐glucokinase antibody. This also had molecular weight equal to that observed extracts, suggesting close similarity...
The effects of bilateral vasectomy on the seminal characteristics were assessed in six bucks Canarian breed. In addition, we tried to establish plasmatic concentrations testosterone and libido bucks. Semen samples collected once a week from 8 weeks before 16 after vasectomy; blood prior vasectomy, then at 1 every 2 4 end experiment. One ejaculated spermatozoa non-motile percentage live was below 5% all vasectomized males; total number cells/ejaculate 3100 x 106 30 control males,...
The functions necessary for normal fertilization to occur in vivo or vitro are examined and a rational approach identifying the main features of fertilizing spermatozoon developed. It is concluded that methods testing quality spermatozoa must probe dynamic changes experienced by during capacitation under stressful incubation conditions. Recent developments multiva hate analysis sperm motility data used illustrate success can be achieved this approach. Ideally, characteristics should...